Isolated Human and Livestock Echinococcus Granulosus Genotypes Using Real-Time Pcr of Cox1 Gene in Northeast Iran

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Isolated Human and Livestock Echinococcus Granulosus Genotypes Using Real-Time Pcr of Cox1 Gene in Northeast Iran Publisher Pubmed

Mohaghegh MA¹ ; Yousofidarani H¹ ; Jafarian AH² ; Mirbadie SR³ ; Fasihiharandi M⁴ ; Ghavimi R⁵ ; Jabalameli Z⁶ ; Azami M⁷ ; Mohammadi M⁸ ; Hejazi SH⁹

Show Affiliations

1. Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2. Department of Pathology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3. School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
4. School of Medicine, Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran
5. Department of Pharmaceutical Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
6. Department of Laboratory Sciences, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
7. Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
8. The Persian Gulf Marine Biotechnology Research Center, Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
9. Department of Parasitology and Mycology, School of Medicine, Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Acta Parasitologica Published:2019

Abstract

Purpose: Cystic echinococcosis (CE), caused by the metacestode of Echinococcus granulosus, is highly endemic over large parts of Iran. The purpose of this study was to investigate the prevalence rate of hydatidosis and mitochondrial cox1 real-time PCR with high-resolution melting curve (HRM) analysis of E. granulosus isolated from human and livestock. Methods: In this cross-sectional study, 61 formalin-fixed paraffin-embedded tissue isolates were collected from human CE cases and 83 hydatid cysts from the liver and lung lesions of the livestock in Khorasan Razavi province, Northeast Iran. DNA was extracted from each isolate and amplified by real-time PCR and analyzed using the HRM method. Results: The HRM analysis using the cox1 gene of 40 E. granulosus human isolates showed that 35 (87.5%), 4 (10%), and 1 (2.5%) of the isolates were categorized as G1, G3, and G6 genotypes, respectively. Out of the total 1342 livestock inspected, 39 (4%) goats and 44(12%) cattle were found harboring hydatid cysts all belonging to E. granulosus sensu stricto. Conclusion: The results confirmed that the high prevalence of E. granulosus sensu stricto in intermediate hosts is remarkable in northeast of Iran coupled with the high prevalence of infection in livestock, which reinforced the need for hydatidosis control programs in this region. © 2019, Witold Stefanski Institute of Parasitology, Polish Academy of Sciences.

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Style	Citing Format
MLA	Mohaghegh MA, et al.. "Isolated Human and Livestock Echinococcus Granulosus Genotypes Using Real-Time Pcr of Cox1 Gene in Northeast Iran." Acta Parasitologica, vol. 64, no. 3, 2019, pp. 679-685.
APA	Mohaghegh MA, Yousofidarani H, Jafarian AH, Mirbadie SR, Fasihiharandi M, Ghavimi R, Jabalameli Z, Azami M, Mohammadi M, Hejazi SH (2019). Isolated Human and Livestock Echinococcus Granulosus Genotypes Using Real-Time Pcr of Cox1 Gene in Northeast Iran. Acta Parasitologica, 64(3), 679-685.
Chicago	Mohaghegh MA, Yousofidarani H, Jafarian AH, Mirbadie SR, Fasihiharandi M, Ghavimi R, Jabalameli Z, Azami M, Mohammadi M, Hejazi SH. "Isolated Human and Livestock Echinococcus Granulosus Genotypes Using Real-Time Pcr of Cox1 Gene in Northeast Iran." Acta Parasitologica 64, no. 3 (2019): 679-685.
Harvard	Mohaghegh MA et al. (2019) 'Isolated Human and Livestock Echinococcus Granulosus Genotypes Using Real-Time Pcr of Cox1 Gene in Northeast Iran', Acta Parasitologica, 64(3), pp. 679-685.
Vancouver	Mohaghegh MA, Yousofidarani H, Jafarian AH, Mirbadie SR, Fasihiharandi M, Ghavimi R, et al.. Isolated Human and Livestock Echinococcus Granulosus Genotypes Using Real-Time Pcr of Cox1 Gene in Northeast Iran. Acta Parasitologica. 2019;64(3):679-685.
BibTex	@article{ author = {Mohaghegh MA and Yousofidarani H and Jafarian AH and Mirbadie SR and Fasihiharandi M and Ghavimi R and Jabalameli Z and Azami M and Mohammadi M and Hejazi SH}, title = {Isolated Human and Livestock Echinococcus Granulosus Genotypes Using Real-Time Pcr of Cox1 Gene in Northeast Iran}, journal = {Acta Parasitologica}, volume = {64}, number = {3}, pages = {679-685}, year = {2019} }
RIS	TY - JOUR AU - Mohaghegh MA AU - Yousofidarani H AU - Jafarian AH AU - Mirbadie SR AU - Fasihiharandi M AU - Ghavimi R AU - Jabalameli Z AU - Azami M AU - Mohammadi M AU - Hejazi SH TI - Isolated Human and Livestock Echinococcus Granulosus Genotypes Using Real-Time Pcr of Cox1 Gene in Northeast Iran JO - Acta Parasitologica VL - 64 IS - 3 SP - 679 EP - 685 PY - 2019 ER -

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