Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Rapid Identification of Echinococcus Granulosus and E. Canadensis Using High-Resolution Melting (Hrm) Analysis by Focusing on a Single Nucleotide Polymorphism Publisher Pubmed



Safa AH1 ; Harandi MF2 ; Tajaddini M3 ; Rostaminejad M5 ; Mohtashamipour M6 ; Pestehchian N4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran
  3. 3. Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Medical Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, Iran
  5. 5. Gastroenterology and Liver Disease Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  6. 6. Department of Medical Parasitology and Mycology, Tabriz University of Medical Sciences, Tabriz, Iran

Source: Japanese Journal of Infectious Diseases Published:2016


Abstract

High-resolution melting (HRM) is a reliable and sensitive scanning method to detect variation in DNA sequences. We used this method to better understand the epidemiology and transmission of Echinococcus granulosus. We tested the use of HRM to discriminate the genotypes of E. granulosus and E. canadensis. One hundred forty-one hydatid cysts were collected from slaughtered animals in different parts of Isfahan-Iran in 2013. After DNA extraction, the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene was amplified using PCR coupled with the HRM curve. The result of HRM analysis using partial the sequences of cox1 gene revealed that 93, 35, and 2 isolates were identified as G1, G3, and G6 genotypes, respectively. A single nucleotide polymorphism (SNP) was found in locus 9867 of the cox1 gene. This is a critical locus for the differentiation between the G6 and G7 genotypes. In the phylogenictree,thesamplewithaSNPwaslocatedbetween the G6 and G7 genotypes, which suggest that this isolate has a G6/G7 genotype. The HRM analysis developed in the present study provides a powerful technique for molecular and epidemiological studies on echinococcosis in humans and animals. © 2016, National Institute of Health. All rights reserved.
Related Docs
View other Related Docs
1. Genetic Diversity of Echinococcus Granulosus in Center of Iran, Korean Journal of Parasitology (2014)
2. First Report of Tasmanian Sheep Strain (G2) Genotype Isolated From Iranian Goat Using the High Resolution Melting (Hrm) Analysis, Gastroenterology and Hepatology from Bed to Bench (2016)
3. Isolated Human and Livestock Echinococcus Granulosus Genotypes Using Real-Time Pcr of Cox1 Gene in Northeast Iran, Acta Parasitologica (2019)
4. Genetic Diversity and Phylogeography of the Elusive, But Epidemiologically Important Echinococcus Granulosus Sensu Stricto Genotype G3, Parasitology (2018)
5. Echinococcus Granulosus Genotypes in Iran, Gastroenterology and Hepatology from Bed to Bench (2014)
6. Analysis of the Cox1 Gene in Echinococcus Granulosus From Sheep in Northeast Iran Using Pcr High-Resolution Melting (Qpcr-Hrm) Curve Analysis, Tropical Biomedicine (2018)
7. Distinguishing Echinococcus Granulosus Sensu Stricto Genotypes G1 and G3 With Confidence: A Practical Guide, Infection, Genetics and Evolution (2018)
8. Global Phylogeography and Genetic Diversity of the Zoonotic Tapeworm Echinococcus Granulosus Sensu Stricto Genotype G1, International Journal for Parasitology (2018)
Experts (# of related papers)
View all Related Experts
Seyed Hossein Mirhendi Esfahani (12)
Hossein Yousofi Darani (10)
Other Related Docs
9. Molecular Studies on Cystic Echinococcosis of Camel (Camelus Dromedarius) and Report of Echinococcus Ortleppi in Iran, Iranian Journal of Parasitology (2017)
10. Genotype Identification of Human Cystic Echinococcosis in Isfahan, Central Iran, Parasitology Research (2010)
11. The Benefits of Analysing Complete Mitochondrial Genomes: Deep Insights Into the Phylogeny and Population Structure of Echinococcus Granulosus Sensu Lato Genotypes G6 and G7, Infection, Genetics and Evolution (2018)
12. Multilocus Sequence Analysis of Echinococcus Granulosus Strains Isolated From Humans and Animals in Iran, Experimental Parasitology (2017)
13. Echinococcus Multilocularis and Echinococcus Granulosus in Canines in North-Khorasan Province, Northeastern Iran, Identified Using Morphology and Genetic Characterization of Mitochondrial Dna, Parasites and Vectors (2019)
14. Analysis of Nad2 and Nad5 Enables Reliable Identification of Genotypes G6 and G7 Within the Species Complex Echinococcus Granulosus Sensu Lato, Infection, Genetics and Evolution (2019)
15. Genetic Characterization of Echinococcus Granulosus Strains Isolated From Humans Based on Nad1 and Cox1 Gene Analysis in Isfahan, Central Iran, Journal of Helminthology (2018)
16. New Mitogenome and Nuclear Evidence on the Phylogeny and Taxonomy of the Highly Zoonotic Tapeworm Echinococcus Granulosus Sensu Stricto, Infection, Genetics and Evolution (2017)
17. Molecular Characterization of Human and Animal Echinococcus Granulosus Isolates in Isfahan, Iran, Acta Tropica (2011)
18. Molecular Phylogeny Based on Six Nuclear Genes Suggests That Echinococcus Granulosus Sensu Lato Genotypes G6/G7 and G8/G10 Can Be Regarded As Two Distinct Species, Parasitology (2018)
19. Molecular Epidemiology of Echinococcus Granulosus Strains in Domestic Herbivores of Lorestan, Iran, Jundishapur Journal of Microbiology (2011)
20. Molecular Identification of Echinococcus Granulosus Sensu Lato by Mitochondrial Cox1 and Ssu-Rdna Markers in Dogs in the West of Iran, Gene Reports (2020)
21. A New Primer Pair in Its1 Region for Molecular Studies on Echinococcus Granulosus, Iranian Journal of Public Health (2007)
22. Echinococcus Granulosus Sheep Strain (G1) As the Predominant Genotype in Definitive Host (Dogs) Isolates in Northeastern Iran, Veterinary Parasitology: Regional Studies and Reports (2024)
23. Sensitive Detection of Specific Cell-Free Dna in Serum Samples From Sheep With Cystic Echinococcosis, PLoS Neglected Tropical Diseases (2023)
24. Histopathological Study on Fibrous Layer of Hydatid Cyst in Camel, Cattle and Sheep, Journal of Isfahan Medical School (2016)
25. Report of a Hydatid Cyst Case With Biceps Brachii Involvement, Advanced Biomedical Research (2019)
26. Discrimination of Paederus Fuscipes and Paederus Littoralis by Mtdna-Coi Pcr-Rflp, Journal of Arthropod-Borne Diseases (2016)
27. Immunological Diagnosis of Human Hydatid Cyst Using Western Immunoblotting Technique, Journal of Research in Medical Sciences (2016)
28. A Survey on Apoptosis and Hydatid Cyst Infertility, Journal of Isfahan Medical School (2021)
29. Diagnostic Value of Hydatid Cyst Antigens Using Western Blotting Method, Jundishapur Journal of Microbiology (2010)
30. Stability Determination of Recombinant Echinococcus Granulosus Antigen B Kit by Physical and Bacteriostatical Methods, Journal of Isfahan Medical School (2013)
31. Laboratory Diagnosis of Human Cystic Echinococcosis, Advances in Medicine and Biology. Volume 136 (2019)
32. Postoperative Recurrence of Hydatid Cyst According to World Health Organization (Who) Classification, Journal of Isfahan Medical School (2018)
33. Diagnostic Value of Echinococcus Granulosus Recombinant Protein B-Containing Kit in Comparison With the Euroimmun Kit, Journal of Isfahan Medical School (2013)
34. Il-4 Gene Expression in Adventitial Layer (Fibrous Layer) of Hepatic Ovine and Bovine Hydatid Cysts, Journal of Parasitic Diseases (2016)
35. Overview of Hydatid Disease in Iranian Children, Archives of Pediatric Infectious Diseases (2015)
36. Mycobacterium Iranicum Sp. Nov., a Rapidly Growing Scotochromogenic Species Isolated From Clinical Specimens on Three Different Continents, International Journal of Systematic and Evolutionary Microbiology (2013)