Significant Association Between Nonsyndromic Cleft Lip With or Without Cleft Palate and Irf6rs2235371 Polymorphism in Iranian Familiar Population Publisher Pubmed



Jafary F^{1, 2, 3, 4} ; Nadeali Z^{1, 2, 3, 4} ; Salehi M^{1, 2, 3, 4} ; Hosseinzadeh M^{1, 2, 3, 4} ; Sedghi M^{1, 2, 3, 4} ; Gholamrezapour T^{1, 2, 3, 4} ; Nouri N^{1, 2, 3, 4} Show Affiliations
Source: Molekuliarnaia biologiia Published:2015

Abstract

According to previous studies the IRF6rs2235371 polymorphism is a risk factor for NSCL/P in different populations. However our recent study revealed no correlation between IRF6rs642961 and NSCL/P in our population. In the present study we have investigated the relationship between IRF6rs2235371 and NSCL/P in same group to determine whether IRF6rs2235371 is a risk factor in our population as well. We analyzed the IRF6rs2235371 genotype in a subset of the Iranian population using the Polymerase Chain Reaction technique. The PCR products were digested with DpnII. Chi-square test was applied to analyze the obtained result. The patients were supplied by the Cleft Lip and Palate Clinic of the Isfahan University of Medical Science. A clinician ascertained the non-syndromic status of all patients and that no clefting drugs, ethanol or smoking were abused during pregnancy. The control group was selected from unaffected subjects with no history of NSCL/P in their families. 107 patients from 107 Iranian unrelated families and 100 controls were screened. There was a significant association between the IRF6rs2235371 genotype sand an increased NSCL/P risk. Our data indicates that the IRF6rs2235371 variation can increase the risk of NSCL/P in the Iranian population. This result is in contrast with the results of our recent study on the correlation between the IRF6rs642961 polymorphism and NSCL/P in the same group.