Anti-Inflammatory Effects of Atorvastatin by Suppressing Traf3ip2 and Il-17Ra in Human Glioblastoma Spheroids Cultured in a Three-Dimensional Model: Possible Relevance to Glioblastoma Treatment

Anti-Inflammatory Effects of Atorvastatin by Suppressing Traf3ip2 and Il-17Ra in Human Glioblastoma Spheroids Cultured in a Three-Dimensional Model: Possible Relevance to Glioblastoma Treatment Publisher Pubmed

Bayat N¹ ; Ebrahimibarough S² ; Norouzijavidan A¹ ; Saberi H¹ ; Ardakan MMM² ; Ai A³ ; Soleimannejad M² ; Ai J^{1, 2}

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1. Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Tissue Engineering and Applied Cell Sciences, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
3. School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Molecular Neurobiology Published:2018

Abstract

Glioblastoma multiform (GBM) is a primary malignant brain tumor with a few therapeutic targets available for it. The interaction between the immune system and glioma is an important factor that could lead to novel therapeutic approaches to fight glioma. In this study, we investigated in vitro anti-inflammatory and apoptotic activity of atorvastatin in different concentrations 1, 5, and 10 μM on glioma spheroid cells cultured in a three-dimensional model in fibrin gel that indicate the complex in vivo microenvironment better than a simple two-dimensional cell culture. A mechanistic insight into the role of IL-17RA, TRAF3IP2, and apoptotic genes in progression of glioma could provide an important way for therapy of malignant tumors with manipulation of this inflammatory axis. To reach for these aims, after 24 and 48 h exposure with different concentrations of atorvastatin, caspase-8, caspase-3, Bcl-2, TRAF3IP2, and IL-17RA gene expression were assayed. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay and cell cycle assay were used for evaluating the cell apoptosis and proliferation. The results showed that atorvastatin has anti-inflammatory and apoptotic effects against glioma spheroids. Atorvastatin induced the expression of caspase-3 and caspase-8 and downregulated the expression of Bcl-2, TRAF3IP2, and IL-17RA especially at 10 μM concentration. These effects are dose dependent. The most likely mechanisms are the inhibition of inflammation by IL-17RA interaction with TRAF3IP2 and NF-κB signaling pathway. Finally, these results suggest that atorvastatin could be used as an anti-cancer agent for glioblastoma treatment. © 2017, Springer Science+Business Media New York.

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Somayeh Ebrahimi Barough (2)

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Style	Citing Format
MLA	Bayat N, et al.. "Anti-Inflammatory Effects of Atorvastatin by Suppressing Traf3ip2 and Il-17Ra in Human Glioblastoma Spheroids Cultured in a Three-Dimensional Model: Possible Relevance to Glioblastoma Treatment." Molecular Neurobiology, vol. 55, no. 3, 2018, pp. 2102-2110.
APA	Bayat N, Ebrahimibarough S, Norouzijavidan A, Saberi H, Ardakan MMM, Ai A, Soleimannejad M, Ai J (2018). Anti-Inflammatory Effects of Atorvastatin by Suppressing Traf3ip2 and Il-17Ra in Human Glioblastoma Spheroids Cultured in a Three-Dimensional Model: Possible Relevance to Glioblastoma Treatment. Molecular Neurobiology, 55(3), 2102-2110.
Chicago	Bayat N, Ebrahimibarough S, Norouzijavidan A, Saberi H, Ardakan MMM, Ai A, Soleimannejad M, Ai J. "Anti-Inflammatory Effects of Atorvastatin by Suppressing Traf3ip2 and Il-17Ra in Human Glioblastoma Spheroids Cultured in a Three-Dimensional Model: Possible Relevance to Glioblastoma Treatment." Molecular Neurobiology 55, no. 3 (2018): 2102-2110.
Harvard	Bayat N et al. (2018) 'Anti-Inflammatory Effects of Atorvastatin by Suppressing Traf3ip2 and Il-17Ra in Human Glioblastoma Spheroids Cultured in a Three-Dimensional Model: Possible Relevance to Glioblastoma Treatment', Molecular Neurobiology, 55(3), pp. 2102-2110.
Vancouver	Bayat N, Ebrahimibarough S, Norouzijavidan A, Saberi H, Ardakan MMM, Ai A, et al.. Anti-Inflammatory Effects of Atorvastatin by Suppressing Traf3ip2 and Il-17Ra in Human Glioblastoma Spheroids Cultured in a Three-Dimensional Model: Possible Relevance to Glioblastoma Treatment. Molecular Neurobiology. 2018;55(3):2102-2110.
BibTex	@article{ author = {Bayat N and Ebrahimibarough S and Norouzijavidan A and Saberi H and Ardakan MMM and Ai A and Soleimannejad M and Ai J}, title = {Anti-Inflammatory Effects of Atorvastatin by Suppressing Traf3ip2 and Il-17Ra in Human Glioblastoma Spheroids Cultured in a Three-Dimensional Model: Possible Relevance to Glioblastoma Treatment}, journal = {Molecular Neurobiology}, volume = {55}, number = {3}, pages = {2102-2110}, year = {2018} }
RIS	TY - JOUR AU - Bayat N AU - Ebrahimibarough S AU - Norouzijavidan A AU - Saberi H AU - Ardakan MMM AU - Ai A AU - Soleimannejad M AU - Ai J TI - Anti-Inflammatory Effects of Atorvastatin by Suppressing Traf3ip2 and Il-17Ra in Human Glioblastoma Spheroids Cultured in a Three-Dimensional Model: Possible Relevance to Glioblastoma Treatment JO - Molecular Neurobiology VL - 55 IS - 3 SP - 2102 EP - 2110 PY - 2018 ER -

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