Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Analysis of Nad2 and Nad5 Enables Reliable Identification of Genotypes G6 and G7 Within the Species Complex Echinococcus Granulosus Sensu Lato Publisher Pubmed



Laurimae T1 ; Kinkar L1 ; Romig T2 ; Umhang G3 ; Casulli A4, 5 ; Omer RA6 ; Sharbatkhori M7 ; Mirhendi H8 ; Poncegordo F9 ; Lazzarini LE10 ; Soriano SV10 ; Varcasia A11 ; Rostaminejad M12 ; Andresiuk V13 Show All Authors
Authors
  1. Laurimae T1
  2. Kinkar L1
  3. Romig T2
  4. Umhang G3
  5. Casulli A4, 5
  6. Omer RA6
  7. Sharbatkhori M7
  8. Mirhendi H8
  9. Poncegordo F9
  10. Lazzarini LE10
  11. Soriano SV10
  12. Varcasia A11
  13. Rostaminejad M12
  14. Andresiuk V13
  15. Maravilla P14
  16. Gonzalez LM15
  17. Dybicz M16
  18. Gawor J17
  19. Sarkunas M18
  20. Snabel V19
  21. Kuzmina T20
  22. Kia EB21
  23. Saarma U1
Show Affiliations
Authors Affiliations
  1. 1. Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Vanemuise 46, Tartu, 51003, Estonia
  2. 2. Institute of Zoology, Parasitology Unit, University of Hohenheim, Stuttgart, 70599, Germany
  3. 3. Anses, Wildlife Surveillance and Eco-epidemiology Unit, National Reference Laboratory for Echinococcus spp., Nancy Laboratory for Rabies and Wildlife, Malzeville, 54220, France
  4. 4. World Health Organization Collaborating Centre for the Epidemiology, Detection and Control of Cystic and Alveolar Echinococcosis (in humans and animals), Istituto Superiore di Sanita, Viale Regina Elena 299, Rome, 00161, Italy
  5. 5. European Union Reference Laboratory for Parasites (EURLP), Istituto Superiore di Sanita, Viale Regina Elena 299, Rome, 00161, Italy
  6. 6. National University Research Institute, National University Sudan, Khartoum, Sudan
  7. 7. Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran
  8. 8. Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  9. 9. Department of Parasitology, Faculty of Pharmacy, Complutense University, Plaza Ramon y Cajal s/n, Madrid, 28040, Spain
  10. 10. Department of Microbiology and Parasitology, Faculty of Medical Sciences, Comahue National University, Buenos Aires, 1400, 8300, Neuquen, Argentina
  11. 11. Laboratorio di Parassitologia e Malattie Parassitarie, Ospedale Didattico Veterinario Dipartimento di Medicina Veterinaria, Universita degli Studi di Sassari, Via Vienna 2, Sassari, 07100, Italy
  12. 12. Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  13. 13. Laboratorio de Zoonosis Parasitarias, FCEyN, UNMdP, Funes 3350, CP: 7600 Mar del Plata, Buenos Aires, Argentina
  14. 14. Hospital General “Dr. Manuel Gea Gonzalez�, Departamento de Ecologia de Agentes Patogenos, 14080, DF, Mexico
  15. 15. Parasitology Department, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, Madrid, 28220, Spain
  16. 16. Department of General Biology and Parasitology, Medical University of Warsaw, 5 Chalubinskiego Str., Warsaw, 02-004, Poland
  17. 17. W. Stefanski Institute of Parasitology, Polish Academy of Science, Twarda51/55, Warsaw, 00-818, Poland
  18. 18. Department of Veterinary Pathobiology, Veterinary Academy, Lithuanian University of Health Sciences, Tilzes Street 18, Kaunas, 47181, Lithuania
  19. 19. Institute of Parasitology, Slovak Academy of Sciences, Kosice, Hlinkova 3, Kosice, 040 01, Slovakia
  20. 20. I.I. Schmalhausen Institute of Zoology, National Academy of Sciences of Ukraine, Kyiv, 01030, Ukraine
  21. 21. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Infection# Genetics and Evolution Published:2019


Abstract

The larval stages of tapeworms in the species complex Echinococcus granulosus sensu lato cause a zoonotic disease known as cystic echinococcosis (CE). Within this species complex, genotypes G6 and G7 are among the most common genotypes associated with human CE cases worldwide. However, our understanding of ecology, biology and epidemiology of G6 and G7 is still limited. An essential first step towards this goal is correct genotype identification, but distinguishing genotypes G6 and G7 has been challenging. A recent analysis based on complete mitogenome data revealed that the conventional sequencing of the cox1 (366 bp) gene fragment mistakenly classified a subset of G7 samples as G6. On the other hand, sequencing complete mitogenomes is not practical if only genotype or haplogroup identification is needed. Therefore, a simpler and less costly method is required to distinguish genotypes G6 and G7. We compared 93 complete mitogenomes of G6 and G7 from a wide geographical range and demonstrate that a combination of nad2 (714 bp) and nad5 (680 bp) gene fragments would be the best option to distinguish G6 and G7. Moreover, this method allows assignment of G7 samples into haplogroups G7a and G7b. However, due to very high genetic variability of G6 and G7, we suggest to construct a phylogenetic network based on the nad2 and nad5 sequences in order to be absolutely sure in genotype assignment. For this we provide a reference dataset of 93 concatenated nad2 and nad5 sequences (1394 bp in total) containing representatives of G6 and G7 (and haplogroups G7a and G7b), which can be used for the reconstruction of phylogenetic networks. © 2019 Elsevier B.V.
Other Related Docs