Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells Using 3D-Printed Pdlla/ Β-Tcp Nanocomposite Scaffolds Publisher



Rezai Rad M1 ; Fahimipour F2, 3 ; Dashtimoghadam E2 ; Nokhbatolfoghahaei H1 ; Tayebi L2 ; Khojasteh A1, 4
Authors

Source: Bioprinting Published:2021


Abstract

Designing bone scaffolds containing both organic and inorganic composites simulating the architecture of the bone is the most important principle in bone tissue engineering. The objective of this study was to fabricate a composite scaffold containing poly (D, L)-lactide (PDLLA) and β-tricalcium phosphate (β-TCP) as a platform for osteogenic differentiation of adipose-derived mesenchymal stem cells. In this study, PDLLA/β-TCP scaffolds were fabricated using three-dimensional printing (3D) technology through melt excursion technique. The physicomechanical characteristics, including microstructure, mechanical properties, of the customized scaffolds were investigated. Further, the in vitro biological characteristics of manufactured scaffolds were evaluated in conjugation with buccal fat pad derived mesenchymal stem cells in terms of cell attachment, viability, proliferation, and osteogenic differentiation capacity. The 3D printed customized scaffold in this study showed proper pore size, porosity, mechanical strength, material composition, biocompatibility, and osteogenic differentiation capacity. The obtained results converge to reveal the promising features of the nanocomposite 3D printed platform for personalized bone tissue engineering. © 2020 Elsevier B.V.
Related Docs
View other Related Docs
1. A Deep Insight Into the Preparation of Ceramic Bone Scaffolds Utilizing Robocasting Technique, Ceramics International (2022)
2. Collagenous Matrix Supported by a 3D-Printed Scaffold for Osteogenic Differentiation of Dental Pulp Cells, Dental Materials (2018)
3. Fabrication of Cancellous Biomimetic Chitosan-Based Nanocomposite Scaffolds Applying a Combinational Method for Bone Tissue Engineering, Journal of Biomedical Materials Research - Part A (2015)
Experts (# of related papers)
View all Related Experts
Mahmoud Azami (5)
Reza Masaeli (1)
Other Related Docs
4. Applying Extrusion-Based 3D Printing Technique Accelerates Fabricating Complex Biphasic Calcium Phosphate-Based Scaffolds for Bone Tissue Regeneration, Journal of Advanced Research (2022)
5. Challenges in Three-Dimensional Printing of Bone Substitutes, Tissue Engineering - Part B: Reviews (2019)
6. Different Porosities of Chitosan Can Influence the Osteogenic Differentiation Potential of Stem Cells, Journal of Cellular Biochemistry (2018)
7. Development of Plga-Coated Β-Tcp Scaffolds Containing Vegf for Bone Tissue Engineering, Materials Science and Engineering C (2016)
8. Mechanical Modeling of Silk Fibroin/Tio2 and Silk Fibroin/Fluoridated Tio2 Nanocomposite Scaffolds for Bone Tissue Engineering, Iranian Polymer Journal (English Edition) (2020)
9. Scalable and Cost-Effective Generation of Osteogenic Micro-Tissues Through the Incorporation of Inorganic Microparticles Within Mesenchymal Stem Cell Spheroids, Biofabrication (2020)
10. Recent Advances on 3D-Printed Pcl-Based Composite Scaffolds for Bone Tissue Engineering, Frontiers in Bioengineering and Biotechnology (2023)
11. Bone Regeneration in Rat Using a Gelatin/Bioactive Glass Nanocomposite Scaffold Along With Endothelial Cells (Huvecs), International Journal of Applied Ceramic Technology (2018)
12. Additive Manufacture of Pcl/Nha Scaffolds Reinforced With Biodegradable Continuous Fibers: Mechanical Properties, In-Vitro Degradation Profile, and Cell Study, European Polymer Journal (2022)
13. Optimisation and Biological Activities of Bioceramic Robocast Scaffolds Provided With an Oxygen-Releasing Coating for Bone Tissue Engineering Applications, Ceramics International (2019)
14. 3D Printed Pcl Scaffold Reinforced With Continuous Biodegradable Fiber Yarn: A Study on Mechanical and Cell Viability Properties, Polymer Testing (2020)
15. Scrophularia Striata Extract Incorporated Nanofibrous Scaffold Improved Osteogenic Differentiation of Mesenchymal Stem Cells, Journal of Drug Delivery Science and Technology (2023)
16. Fabrication and Characterization of 3D Collagen/ Polycaprolactone/ Hydroxyapatite Nanoparticles Scaffold Using 3D Printing Method for Repairing Damaged Bone Tissue, International Journal of Engineering, Transactions B: Applications (2025)
17. Fabrication of a Three-Dimensional Β-Tricalcium-Phosphate/Gelatin Containing Chitosan-Based Nanoparticles for Sustained Release of Bone Morphogenetic Protein-2: Implication for Bone Tissue Engineering, Materials Science and Engineering C (2017)
18. Preparation and Characterization of 3D Nanocomposite Scaffold From Bioactive Glass/Β-Tricalcium Phosphate Via Robocasting Method for Bone Tissue Engineering, Journal of Non-Crystalline Solids (2022)
19. Biomimetic 3D-Printed Pcl Scaffold Containing a High Concentration Carbonated-Nanohydroxyapatite With Immobilized-Collagen for Bone Tissue Engineering: Enhanced Bioactivity and Physicomechanical Characteristics, Biomedical Materials (Bristol) (2021)
20. Pre-Vascularized Porous Gelatin-Coated Β-Tricalcium Phosphate Scaffolds for Bone Regeneration: An in Vivo and in Vitro Investigation, In Vitro Cellular and Developmental Biology - Animal (2025)