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Incorporation of Montmorillonite Into Microfluidics-Generated Chitosan Microfibers Enhances Neuron-Like Pc12 Cells for Application in Neural Tissue Engineering Publisher Pubmed



Katoli Z1, 2 ; Navaeinigjeh M2, 3 ; Mirzababaei S2 ; Sabahi H1 ; Baeeri M4 ; Akrami M3, 5 ; Roshanbinfar K6 ; Engel FB6 ; Abdollahi M4, 7
Authors
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Authors Affiliations
  1. 1. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran
  2. 2. Pharmaceutical Sciences Research Center (PSRC), Tehran University of Medical Sciences (TUMS), Tehran, Iran
  3. 3. Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences (TUMS), Tehran, Iran
  4. 4. Toxicology and Diseases Group (TDG), Pharmaceutical Sciences Research Center (PSRC), Tehran University of Medical Sciences (TUMS), Iran
  5. 5. Institute of Biomaterials, University of Tehran, Tehran University of Medical Sciences (IBUTUMS), Tehran, Iran
  6. 6. Experimental Renal and Cardiovascular Research, Department of Nephropathology, Institute of Pathology, Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Erlangen, Germany
  7. 7. Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: Carbohydrate Polymers Published:2024


Abstract

The complexity in structure and function of the nervous system, as well as its slow rate of regeneration, makes it more difficult to treat it compared to other tissues. Neural tissue engineering aims to create an appropriate environment for nerve cell proliferation and differentiation. Fibrous scaffolds with suitable morphology and topography and better mimicry of the extracellular matrix have been promising for the alignment and migration of neural cells. On this premise, to improve the properties of the scaffold, we combined montmorillonite (MMT) with chitosan (CS) polymer and created microfibers with variable diameters and varied concentrations of MMT using microfluidic technology and tested its suitability for the rat pheochromocytoma cell line (PC12). According to the findings, CS/MMT 0.1 % compared to CS/MMT 0 % microfibers showed a 201 MPa increase in Young's modulus, a 68 mS/m increase in conductivity, and a 1.4-fold increase in output voltage. Analysis of cell mitochondrial activity verified the non-toxicity, resulting in good cell morphology with orientation along the microfiber. Overall, the results of this project showed that with a low concentration of MMT, the properties of microfibers can be significantly improved and a suitable scaffold can be designed for neural tissue engineering. © 2024
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