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An Electrochemical Biosensor Based on Cobalt Nanoparticles Synthesized in Iron Storage Protein Molecules to Determine Ascorbic Acid Publisher Pubmed



Rafipour R1, 2 ; Kashanian S3 ; Hashemi S4 ; Shahabadi N5, 6 ; Omidfar K1, 7
Authors
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Authors Affiliations
  1. 1. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Chemistry, College of Science, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
  3. 3. Faculty of Chemistry, Sensor and Biosensor Research Center (SBRC) & Nanoscience and Nanotechnology Research Center (NNRC), Razi University, Kermanshah, Iran
  4. 4. Department of Animal Science, Faculty of Agriculture, University of Tehran, Karaj, Iran
  5. 5. Department of Inorganic Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran
  6. 6. Medical Biology Research Center (MBRC), Kermanshah University of Medical Sciences, Kermanshah, Iran
  7. 7. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Biotechnology and Applied Biochemistry Published:2016


Abstract

The electrochemical detection of ascorbic acid (AA) was investigated using a cobalt(III)–ferritin immobilized on a self-assembled monolayer modified gold electrode in phosphate buffer solution (pH 7.5). The modified electrode showed excellent electrochemical activity for oxidation of AA. The response to AA on the modified electrode was examined using cyclic and differential pulse voltammetry techniques. The resulting biosensor showed a linear response to AA in a concentration range from 6.25×10−6 to 2.31×10−5 M with sensitivity of 86,437 μAM− 1 and detection limit of 4.65 × 10−6 M based on a signal-to-noise ratio of 3. Electrochemical parameters including the charge transfer coefficient (α) and the apparent heterogeneous electron transfer rate constant (ks) for AA were found to be 0.52 and 1.054 Sec−1, respectively. It has been shown that, using this modified electrode, AA can be determined with high sensitivity, low detection limit, and high selectivity. © 2015 International Union of Biochemistry and Molecular Biology, Inc.