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Human Umbilical Cord Mesenchymal Stem Cells Express Cholinergic Neuron Markers During Co-Culture With Amniotic Membrane Cells and Retinoic Acid Induction Publisher



Kouchakian MR1 ; Koruji M1, 2 ; Najafi M3 ; Moniri SF4 ; Asgari A5 ; Shariatpanahi M6 ; Moosavi SA7 ; Asgari HR1, 2, 7
Authors
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Authors Affiliations
  1. 1. Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Stem Cell and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. School of Pharmacy, Zanjan University of medical sciences, Zanjan, Iran
  6. 6. Department of Toxicology & Pharmacology, School of Pharmacy, International Campus, Iran University of Medical Sciences, Tehran, Iran
  7. 7. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran

Source: Medical Journal of the Islamic Republic of Iran Published:2021


Abstract

Background: A wide variety of cytokines are released from human amniotic membrane cells (hAMCs), which can increase the rate of differentiation of mesenchymal stem cell into the neurons. We studied the effect of Retinoic Acid (RA) on the differentiation rate of human Umbilical Cord Mesenchymal Stem Cells (hUMSCs) which were co-cultured with hAMCs. Methods: In this experimental study, both hUMSCs and hAMCs were isolated from postpartum human umbilical cords and placenta respectively. The expression of mesenchymal (CD73, CD90 and CD105), hematopoietic and endothelial (CD34 and CD45) markers in hUMSCs were confirmed by flow cytomet y. The hUMSCs were cultured in four distinct groups: group 1) Control, group 2) Co-culture with hAMCs, group 3) RA treatment and group 4) Co-culture w th hAMCs treated by RA. Twelve days after culturing, the expression of NSE, MAP2 and ChAT differentiation genes and their related proteins were examined by real-time PCR and immunocytochemistry respectively. Results: The flow-cytometry analysis indicated increased expression of mesenchymal markers and a low expression of both hematopoietic and endothelial markers (CD73:98.24%, CD90: 97.32%, CD105: 90.75%, CD34: 2.96%, and CD45:1.74%). Moreover, the expression of both NSE and MAP2 markers was increased significantly in all studi d groups in comparison to the control group On the other hand, the expression of ChAT had a significant increase in the group 2 and 4 (RA and RA+ co-culture). Conclusion: RA can be used as an effective inducer to differentiate hUMSCs into cho inergic-like cells, and hAMCs could increase the number of differentiated cells as an effective factor. © Iran University of Medical Sciences
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