Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Effect of Gestational Age on Migration Ability of the Human Umbilical Cord Vein Mesenchymal Stem Cells Publisher Pubmed



Mohammadi M1, 2 ; Mohammadi M1, 2 ; Rezaee MA4, 5 ; Ghadimi T6 ; Abolhasani M1, 2 ; Rahmani MR4, 5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran
  2. 2. Department of Immunology and Hematology, Faculty of Paramedical, Kurdistan University of Medical Sciences, Sanandaj, Iran
  3. 3. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Zoonosis Research center, Kurdistan University of Medical Sciences, Sanandaj, Iran
  5. 5. Department of Medical Laboratory Sciences, Faculty of Paramedicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
  6. 6. Department of Surgery, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran

Source: Advances in Medical Sciences Published:2018


Abstract

Purpose Migration ability of mesenchymal stem cells (MSCs) towards chemotactic mediators is a determinant factor in cell therapy. MSCs derived from different sources show different properties. Here we compared the migration ability of the term and the pre-term human umbilical cord vein MSCs (hUCV-MSCs). Materials/Methods MSCs were isolated from term and pre-term umbilical cord vein, and cultured to passage 3–4. Migration rate of both groups was assessed in the presence of 10% FBS using chemotaxis assay. Surface expression of CXCR4 was measured by flow cytometery. The relative gene expression of CXCR4, IGF1-R, PDGFRα MMP-2, MMP-9, MT1-MMP and TIMP-2 were evaluated using real time PCR. Results The isolation rate of the pre-term hUCV-MSCs was higher than the term hUCV-MSCs. Phenotype characteristics and differentiation ability of the term and pre-term hUCV-MSCs were not different. The migration rate of the pre-term hUCV-MSCs was more than the term hUCV-MSCs. Gene and surface expressions of the CXCR4 were both significantly higher in the pre-term hUCV-MSCs (P ≤ 0.05). The mRNA levels of PDGFRα MMP-2, MMP-9, MT1-MMP and TIMP-2 showed no significant difference between the two groups. Conclusion Our results showed that the gestational age can affect the migration ability of the hUCV-MSCs. © 2017 Medical University of Bialystok
Related Docs
View other Related Docs
1. Mesenchymal Stromal Cells and Car-T Cells in Regenerative Medicine: The Homing Procedure and Their Effective Parameters, European Journal of Haematology (2024)
2. Effect of 17Β-Estradiol on Mediators Involved in Mesenchymal Stromal Cell Trafficking in Cell Therapy of Diabetes, Cytotherapy (2015)
3. Germline Cells Derived From Mesenchymal Stem Cells, With the Focus on Wharton's Jelly, Asian Pacific Journal of Reproduction (2018)
4. Immunomodulatory Properties of Umbilical Cord Vein Mesenchymal Stromal Cells Influenced by Gestational Age and in Vitro Expansion, Immunology Letters (2018)
5. Biological Aspects and Clinical Applications of Mesenchymal Stem Cells: Key Features You Need to Be Aware Of, Current Pharmaceutical Biotechnology (2021)
6. Mesenchymal Stem Cells in the Treatment of Amyotrophic Lateral Sclerosis, Current Stem Cell Research and Therapy (2016)
7. Application of Mesenchymal Stem Cells in Melanoma: A Potential Therapeutic Strategy for Delivery of Targeted Agents, Current Medicinal Chemistry (2016)
8. Regenerative Medicine for the Treatment of Ischemic Heart Disease; Status and Future Perspectives, Frontiers in Cell and Developmental Biology (2021)
Experts (# of related papers)
View all Related Experts
Bagher Larijani (5)
Babak Arjmand (4)
Other Related Docs
9. A Simple, Rapid, and Efficient Method for Isolating Mesenchymal Stem Cells From the Entire Umbilical Cord, Cell Transplantation (2016)
10. Transplantation of Fetal Stem Cells: A New Horizon for Treatment of Degenerative Diseases, Iranian Journal of Public Health (2015)
11. Promotion of Cell-Based Therapy: Special Focus on the Cooperation of Mesenchymal Stem Cell Therapy and Gene Therapy for Clinical Trial Studies, Advances in Experimental Medicine and Biology (2018)
12. Nanotechnology and Bioengineering Approaches to Improve the Potency of Mesenchymal Stem Cell As an Off-The-Shelf Versatile Tumor Delivery Vehicle, Medicinal Research Reviews (2024)
13. Paracrine Effects of Mesenchymal Stem Cells in Ischemic Stroke: Opportunities and Challenges, Molecular Neurobiology (2022)
14. Novel Cell-Based Therapies in Inflammatory Bowel Diseases: The Established Concept, Promising Results, Human Cell (2021)
15. Genetically Engineered Mesenchymal Stromal Cells As a New Trend for Treatment of Severe Acute Graft-Versus-Host Disease, Clinical and Experimental Immunology (2022)
16. Retinoic Acid As the Stimulating Factor for Differentiation of Wharton’S Jelly– Mesenchymal Stem Cells Into Hepatocyte-Like Cells, Avicenna Journal of Medical Biotechnology (2015)
17. Therapeutic Role of Mesenchymal Stem Cell-Derived Exosomes in Respiratory Disease, Stem Cell Research and Therapy (2022)
18. The Role of Mesenchymal Stem Cells in Atherosclerosis: Prospects for Therapy Via the Modulation of Inflammatory Milieu, Journal of Clinical Medicine (2019)
19. Genetically Engineered Mesenchymal Stem Cells: Targeted Delivery of Immunomodulatory Agents for Tumor Eradication, Cancer Gene Therapy (2020)
20. Genetically-Modified Stem Cell in Regenerative Medicine and Cancer Therapy; a New Era, Current Gene Therapy (2022)
21. Combination Therapy of Sorafenib With Mesenchymal Stem Cells As a Novel Cancer Treatment Regimen in Xenograft Models of Hepatocellular Carcinoma, Journal of Cellular Physiology (2019)
22. Crispr/Cas9-Engineered Mesenchymal Stromal/Stem Cells and Their Extracellular Vesicles: A New Approach to Overcoming Cell Therapy Limitations, Biomedicine and Pharmacotherapy (2022)
23. Co-Transplantation of Human Fetal Mesenchymal and Hematopoietic Stem Cells in Type 1 Diabetic Mice Model, Frontiers in Endocrinology (2019)
24. Multipotent Stem Cell and Current Application, Acta Medica Iranica (2017)
25. Mesenchymal Stem Cells: Rising Concerns Over Their Application in Treatment of Type One Diabetes Mellitus, Journal of Diabetes Research (2015)
26. Bone Tissue Engineering Using Human Cells: A Comprehensive Review on Recent Trends, Current Prospects, and Recommendations, Applied Sciences (Switzerland) (2019)
27. Manipulated Mesenchymal Stem Cells Applications in Neurodegenerative Diseases, International Journal of Stem Cells (2020)
28. Bone Marrow Mesenchymal Stromal Cells for Diabetes Therapy: Touch, Fuse, and Fix?, Stem Cell Research and Therapy (2022)
29. A Simple and Cost-Effective Method for Isolation and Expansion of Human Fetal Pancreas Derived Mesenchymal Stem Cells, Archives of Iranian Medicine (2015)
30. Prospects for Manipulation of Mesenchymal Stem Cells in Tumor Therapy: Anti-Angiogenesis Property on the Spotlight, International Journal of Stem Cells (2021)
31. Induction of Human Umbilical Wharton’S Jelly-Derived Mesenchymal Stem Cells Toward Motor Neuron-Like Cells, In Vitro Cellular and Developmental Biology - Animal (2015)
32. Anti-Inflammatory Effect of Mesenchymal Stem Cells on Hepatocellular Carcinoma in the Xenograft Mice Model, Veterinary Medicine and Science (2022)
33. Mesenchymal Stem Cell Transplantation in Newly Diagnosed Type-1 Diabetes Patients: A Phase I/Ii Randomized Placebo-Controlled Clinical Trial, Stem Cell Research and Therapy (2022)
34. Therapeutic Abortion and Ectopic Pregnancy: Alternative Sources for Fetal Stem Cell Research and Therapy in Iran As an Islamic Country, Cell and Tissue Banking (2019)
35. Biophysical Evaluation of Treating Adipose Tissue-Derived Stem Cells Using Non-Thermal Atmospheric Pressure Plasma, Scientific Reports (2022)
36. The Clinical Trials of Mesenchymal Stromal Cells Therapy, Stem Cells International (2021)
37. Mesenchymal Stem Cells: A New Platform for Targeting Suicide Genes in Cancer, Journal of Cellular Physiology (2018)
38. A Focus on Allogeneic Mesenchymal Stromal Cells As a Versatile Therapeutic Tool for Treating Multiple Sclerosis, Stem Cell Research and Therapy (2021)
39. Wharton's Jelly Derived Mesenchymal Stem Cells Differentiate Into Oocyte Like Cells in Vitro by Follicular Fluid and Cumulus Cells Conditioned Medium, Heliyon (2020)
40. Therapeutic and Immunomodulatory Potentials of Mesenchymal Stromal/Stem Cells and Immune Checkpoints Related Molecules, Biomarker Research (2024)
41. The Role of Spirulina Platensis on the Proliferation of Rat Bone Marrow-Derived Mesenchymal Stem Cells, Iranian Journal of Microbiology (2023)
42. Mesenchymal Stem Cells and Natural Killer Cells Interaction Mechanisms and Potential Clinical Applications, Stem Cell Research and Therapy (2022)
43. Mesenchymal Stem Cell Mediated Effects on Microglial Phenotype in Cuprizone-Induced Demyelination Model, Journal of Cellular Biochemistry (2019)
44. Application of Mesenchymal Stem Cells (Mscs) in Neurodegenerative Disorders: History, Findings, and Prospective Challenges, Pathology Research and Practice (2023)
45. Overview of Mesenchymal Stem Cells, Stem Cells in Urology (2020)
46. Human Umbilical Cord Mesenchymal Stem Cells Express Cholinergic Neuron Markers During Co-Culture With Amniotic Membrane Cells and Retinoic Acid Induction, Medical Journal of the Islamic Republic of Iran (2021)