Real-Time Quantitative Reverse Transcription-Pcr Analysis of Expression Stability of Aggregatibacter Actinomycetemcomitans Fimbria-Associated Gene in Response to Photodynamic Therapy

Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):

Share this content! On (X network) By

Real-Time Quantitative Reverse Transcription-Pcr Analysis of Expression Stability of Aggregatibacter Actinomycetemcomitans Fimbria-Associated Gene in Response to Photodynamic Therapy Publisher Pubmed

Pourhajibagher M¹ ; Monzavi A² ; Chiniforush N² ; Monzavi MM³ ; Sobhani S³ ; Shahabi S² ; Bahador A^{1, 2, 4}

Show Affiliations

1. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Laser Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
3. School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
4. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Photodiagnosis and Photodynamic Therapy Published:2017

Abstract

Background Aggregatibacter actinomycetemcomitans is an etiological agent of both chronic and aggressive periodontitis. Dissemination of A. actinomycetemcomitans from the oral cavity and initiation of systemic infections has led to new approaches for treatment being needed. In this study, a series of experiments presented investigated the effect of methylene blue (MB)-mediated antimicrobial photodynamic therapy (aPDT) on cell viability and expression of fimbria-associated gene (rcpA) in A. actinomycetemcomitans. Materials and methods To determine the dose-depended effects of aPDT, A. actinomycetemcomitans ATCC 33384 strain photosensitized with MB was irradiated with diode laser following bacterial viability measurements. Cell-surviving assay and expression ratio of rcpA were assessed by colony forming unit and real-time quantitative reverse transcription-PCR (qRT-PCR) assays, respectively. Results In the current study, MB-mediated aPDT using 100 μg/mL showed significant reduction in A. actinomycetemcomitans growth when compared to the control (P < 0.05). Sub-lethal dose of aPDT against A. actinomycetemcomitans was 25 μg/mL MB at fluency of 93.75 J/cm2. Sub-lethal dose of aPDT could lead to about four-fold suppression of expression of rcpA. Conclusion High doses of MB-mediated aPDT could potentially exhibit antimicrobial activity, and the expression of rcpA as an important virulence factor of this strain is reduced in cells surviving aPDT with MB. So, aPDT can be a valuable tool for the treatment of A. actinomycetemcomitans infections. © 2017 Elsevier B.V.

Related Docs

View other Related Docs

1. Monitoring Gene Expression of Rcpa From Aggregatibacter Actinomycetemcomitans Versus Antimicrobial Photodynamic Therapy by Relative Quantitative Real-Time Pcr, Photodiagnosis and Photodynamic Therapy (2017)

2. The Evaluation of Cultivable Microbiota Profile in Patients With Secondary Endodontic Infection Before and After Photo-Activated Disinfection, Photodiagnosis and Photodynamic Therapy (2017)

3. Diagnostic Accuracy of Multiplex Real-Time Pcr Approaches Compared With Cultivation -Based Detection Methods: Monitoring the Endopathogenic Microbiota Pre and Post Photo-Activated Disinfection, Photodiagnosis and Photodynamic Therapy (2018)

4. Exploring Different Photosensitizers to Optimize Elimination of Planktonic and Biofilm Forms of Enterococcus Faecalis From Infected Root Canal During Antimicrobial Photodynamic Therapy, Photodiagnosis and Photodynamic Therapy (2018)

5. Potentiation Effects of Antimicrobial Photodynamic Therapy on Quorum Sensing Genes Expression: A Promising Treatment for Multi-Species Bacterial Biofilms in Burn Wound Infections, Photodiagnosis and Photodynamic Therapy (2020)

6. The Effect of Antimicrobial Photodynamic Therapy on the Expression of Novel Methicillin Resistance Markers Determined Using Cdna-Aflp Approach in Staphylococcus Aureus, Photodiagnosis and Photodynamic Therapy (2017)

7. Expression Patterns of Oxyr Induced by Oxidative Stress From Porphyromonas Gingivalis in Response to Photo-Activated Disinfection, Infection and Drug Resistance (2018)

8. Dual Wavelength Irradiation Antimicrobial Photodynamic Therapy Using Indocyanine Green and Metformin Doped With Nano-Curcumin As an Efficient Adjunctive Endodontic Treatment Modality, Photodiagnosis and Photodynamic Therapy (2020)

Experts (# of related papers)

View all Related Experts

Abbas Bahador (67)

Maryam Pourhajibagher (66)

Other Related Docs

9. Photo-Activated Disinfection Based on Indocyanine Green Against Cell Viability and Biofilm Formation of Porphyromonas Gingivalis, Photodiagnosis and Photodynamic Therapy (2017)

10. Investigation of Arginine A-Specific Cysteine Proteinase Gene Expression Profiling in Clinical Porphyromonas Gingivalis Isolates Against Photokilling Action of the Photo-Activated Disinfection, Lasers in Medical Science (2018)

11. An in Vivo Evaluation of Microbial Diversity Before and After the Photo-Activated Disinfection in Primary Endodontic Infections: Traditional Phenotypic and Molecular Approaches, Photodiagnosis and Photodynamic Therapy (2018)

12. Modulation of Virulence in Enterococcus Faecalis Cells Surviving Antimicrobial Photodynamic Inactivation With Reduced Graphene Oxide-Curcumin: An Ex Vivo Biofilm Model, Photodiagnosis and Photodynamic Therapy (2020)

13. Exploring Photoactivated Disinfection-Induced Bystander Effects on Microbial Biofilms of Aggregatibacter Actinomycetemcomitans, Infectious Disorders - Drug Targets (2021)

14. Gene Expression Profiling of Fima Gene Encoding Fimbriae Among Clinical Isolates of Porphyromonas Gingivalis in Response to Photo-Activated Disinfection Therapy, Photodiagnosis and Photodynamic Therapy (2017)

15. The Efficacy of Photodynamic and Photothermal Therapy on Biofilm Formation of Streptococcus Mutans: An in Vitro Study, Photodiagnosis and Photodynamic Therapy (2017)

16. Analysis of Glucosyltransferase Gene Expression of Clinical Isolates of Streptococcus Mutans Obtained From Dental Plaques in Response to Sub-Lethal Doses of Photoactivated Disinfection, Photodiagnosis and Photodynamic Therapy (2018)

17. In Silico Identification of a Therapeutic Target for Photo-Activated Disinfection With Indocyanine Green: Modeling and Virtual Screening Analysis of Arg-Gingipain From Porphyromonas Gingivalis, Photodiagnosis and Photodynamic Therapy (2017)

18. The Effect of Indocyanine Green Loaded on a Novel Nano-Graphene Oxide for High Performance of Photodynamic Therapy Against Enterococcus Faecalis, Photodiagnosis and Photodynamic Therapy (2017)

19. Antimicrobial Action of Photoactivated C-Phycocyanin Against Enterococcus Faecalis Biofilms: Attenuation of Quorum-Sensing System, Photodiagnosis and Photodynamic Therapy (2019)

20. The Effect of Indocyanine Green Antimicrobial Photothermal/Photodynamic Therapy on the Expression of Bcl-2 and Bax Messenger Rna Levels in Human Gingival Fibroblast Cells; [Влияние Антимикробной Фототермической / Фотодинамической Терапии Индоцианиновым Зелёным На Экспрессию Уровней Bcl-2 И Bax В Матричной Рнк В Клетках Фибробластов Десны Человек], Folia Medica (2020)

21. Comparison of Different Modes of Photo-Activated Disinfection Against Porphyromonas Gingivalis: An in Vitro Study, Photodiagnosis and Photodynamic Therapy (2020)

22. Ex Vivo Assessment of Synergic Effect of Chlorhexidine for Enhancing Antimicrobial Photodynamic Therapy Efficiency on Expression Patterns of Biofilm-Associated Genes of Enterococcus Faecalis, Photodiagnosis and Photodynamic Therapy (2018)

23. Antimicrobial Activity of Photodynamic Therapy in Combination With Colistin Against a Pan-Drug Resistant Acinetobacter Baumannii Isolated From Burn Patient, Photodiagnosis and Photodynamic Therapy (2017)

24. Comparison of Bacterial Disinfection Efficacy Using Blue and Red Lights on Dental Implants Contaminated With Aggregatibacter Actinomycetemcomitans, Photodiagnosis and Photodynamic Therapy (2022)

25. The Impact of Aggregatibacter Actinomycetemcomitans Biofilm-Derived Effectors Following Antimicrobial Photodynamic Therapy on Cytokine Production in Human Gingival Fibroblasts, Photodiagnosis and Photodynamic Therapy (2019)

26. The in Vitro Effect of Antimicrobial Photodynamic Therapy With Indocyanine Green on Enterococcus Faecalis: Influence of a Washing Vs Non-Washing Procedure, Photodiagnosis and Photodynamic Therapy (2016)

27. Photoexcitation Triggering Via Semiconductor Graphene Quantum Dots by Photochemical Doping With Curcumin Versus Perio-Pathogens Mixed Biofilms, Photodiagnosis and Photodynamic Therapy (2019)

28. The Effect of Sublethal Photodynamic Therapy on the Expression of Enterococcal Surface Protein (Esp) Encoding Gene in Enterococcus Faecalis: Quantitative Real-Time Pcr Assessment, Photodiagnosis and Photodynamic Therapy (2018)

29. Antimicrobial Photodynamic Therapy: An Effective Alternative Approach to Control Bacterial Infections, Journal of Lasers in Medical Sciences (2018)

30. Outer Membrane Protein 100 of Aggregatibacter Actinomycetemcomitans Act As a Biopharmaceutical Target for Photodynamic Therapy: An in Silico Analysis, Photodiagnosis and Photodynamic Therapy (2016)

31. Carnosine-Graphene Oxide Conjugates Decorated With Hydroxyapatite As Promising Nanocarrier for Icg Loading With Enhanced Antibacterial Effects in Photodynamic Therapy Against Streptococcus Mutans, Journal of Photochemistry and Photobiology B: Biology (2018)

32. Antimicrobial Efficacy of Photodynamic Therapy Using Two Different Light Sources on the Titanium-Adherent Biofilms of Aggregatibacter Actinomycetemcomitans: An in Vitro Study, Photodiagnosis and Photodynamic Therapy (2019)

33. Dna-Aptamer-Nanographene Oxide As a Targeted Bio-Theragnostic System in Antimicrobial Photodynamic Therapy Against Porphyromonas Gingivalis, Scientific Reports (2022)

34. Antimicrobial Photodynamic Therapy Using Diode Laser Activated Indocyanine Green As an Adjunct in the Treatment of Chronic Periodontitis: A Randomized Clinical Trial, Photodiagnosis and Photodynamic Therapy (2016)

35. Efficacy of Antimicrobial Photodynamic Therapy for Elimination of Aggregatibacter Actinomycetemcomitans Biofilm on Laser-Lok Titanium Discs, Photodiagnosis and Photodynamic Therapy (2019)

36. Monitoring of Virulence Factors and Metabolic Activity in Aggregatibacter Actinomycetemcomitans Cells Surviving Antimicrobial Photodynamic Therapy Via Nano-Chitosan Encapsulated Indocyanine Green, Frontiers in Physics (2018)

37. The Effect of Antimicrobial Photodynamic Therapy Against Virulence Genes Expression in Colistin-Resistance Acinetobacter Baumannii, Laser Therapy (2019)

38. Anti-Biofilm and Anti-Metabolic Effects of Antimicrobial Photodynamic Therapy Using Chlorophyllin-Phycocyanin Mixture Against Streptococcus Mutans in Experimental Biofilm Caries Model on Enamel Slabs, Photodiagnosis and Photodynamic Therapy (2020)

39. Sonodynamic Excitation of Nanomicelle Curcumin for Eradication of Streptococcus Mutans Under Sonodynamic Antimicrobial Chemotherapy: Enhanced Anti-Caries Activity of Nanomicelle Curcumin, Photodiagnosis and Photodynamic Therapy (2020)

40. Photodisinfection Effects of Silver Sulfadiazine Nanoliposomes Doped-Curcumin on Acinetobacter Baumannii: A Mouse Model, Nanomedicine (2020)

41. The Effect of Antimicrobial Photodynamic Therapy on the Expression of Biofilm Associated Genes In Staphylococcus Aureus Strains Isolated From Wound Infections in Burn Patients, Photodiagnosis and Photodynamic Therapy (2019)

42. The Effect of Antimicrobial Photodynamic Therapy Using Chlorophyllin-Phycocyanin Mixture on Enterococcus Faecalis: The Influence of Different Light Sources, Applied Sciences (Switzerland) (2020)

43. An Experimental Study for Rapid Detection and Quantification of Endodontic Microbiota Following Photo-Activated Disinfection Via New Multiplex Real-Time Pcr Assay, Photodiagnosis and Photodynamic Therapy (2018)

44. Evaluation of the Crystal Structure of a Fimbrillin (Fima) From Porphyromonas Gingivalis As a Therapeutic Target for Photo-Activated Disinfection With Toluidine Blue O, Photodiagnosis and Photodynamic Therapy (2017)

45. Changes of Microbial Cell Survival, Metabolic Activity, Efflux Capacity, and Quorum Sensing Ability of Aggregatibacter Actinomycetemcomitans Due to Antimicrobial Photodynamic Therapy-Induced Bystander Effects, Photodiagnosis and Photodynamic Therapy (2019)

46. Photosensitizers in Antibacterial Photodynamic Therapy: An Overview, Laser Therapy (2018)

47. Antimicrobial Photodynamic Therapy: Modern Technology in the Treatment of Wound Infections in Patients With Burns, Journal of Wound Care (2023)

48. Evaluation of Temperature Change During Antimicrobial Photodynamic Therapy With Two Different Photosensitizers in Dental Caries, Photodiagnosis and Photodynamic Therapy (2016)

49. Physico-Mechanical and Antimicrobial Properties of an Orthodontic Adhesive Containing Cationic Curcumin Doped Zinc Oxide Nanoparticles Subjected to Photodynamic Therapy, Photodiagnosis and Photodynamic Therapy (2019)

50. Periodontal Ligament Stem Cell-Derived Exosome-Loaded Emodin Mediated Antimicrobial Photodynamic Therapy Against Cariogenic Bacteria, BMC Oral Health (2024)

Style	Citing Format
MLA	Pourhajibagher M, et al.. "Real-Time Quantitative Reverse Transcription-Pcr Analysis of Expression Stability of Aggregatibacter Actinomycetemcomitans Fimbria-Associated Gene in Response to Photodynamic Therapy." Photodiagnosis and Photodynamic Therapy, vol. 18, no. , 2017, pp. 78-82.
APA	Pourhajibagher M, Monzavi A, Chiniforush N, Monzavi MM, Sobhani S, Shahabi S, Bahador A (2017). Real-Time Quantitative Reverse Transcription-Pcr Analysis of Expression Stability of Aggregatibacter Actinomycetemcomitans Fimbria-Associated Gene in Response to Photodynamic Therapy. Photodiagnosis and Photodynamic Therapy, 18(), 78-82.
Chicago	Pourhajibagher M, Monzavi A, Chiniforush N, Monzavi MM, Sobhani S, Shahabi S, Bahador A. "Real-Time Quantitative Reverse Transcription-Pcr Analysis of Expression Stability of Aggregatibacter Actinomycetemcomitans Fimbria-Associated Gene in Response to Photodynamic Therapy." Photodiagnosis and Photodynamic Therapy 18, no. (2017): 78-82.
Harvard	Pourhajibagher M et al. (2017) 'Real-Time Quantitative Reverse Transcription-Pcr Analysis of Expression Stability of Aggregatibacter Actinomycetemcomitans Fimbria-Associated Gene in Response to Photodynamic Therapy', Photodiagnosis and Photodynamic Therapy, 18(), pp. 78-82.
Vancouver	Pourhajibagher M, Monzavi A, Chiniforush N, Monzavi MM, Sobhani S, Shahabi S, et al.. Real-Time Quantitative Reverse Transcription-Pcr Analysis of Expression Stability of Aggregatibacter Actinomycetemcomitans Fimbria-Associated Gene in Response to Photodynamic Therapy. Photodiagnosis and Photodynamic Therapy. 2017;18():78-82.
BibTex	@article{ author = {Pourhajibagher M and Monzavi A and Chiniforush N and Monzavi MM and Sobhani S and Shahabi S and Bahador A}, title = {Real-Time Quantitative Reverse Transcription-Pcr Analysis of Expression Stability of Aggregatibacter Actinomycetemcomitans Fimbria-Associated Gene in Response to Photodynamic Therapy}, journal = {Photodiagnosis and Photodynamic Therapy}, volume = {18}, number = {}, pages = {78-82}, year = {2017} }
RIS	TY - JOUR AU - Pourhajibagher M AU - Monzavi A AU - Chiniforush N AU - Monzavi MM AU - Sobhani S AU - Shahabi S AU - Bahador A TI - Real-Time Quantitative Reverse Transcription-Pcr Analysis of Expression Stability of Aggregatibacter Actinomycetemcomitans Fimbria-Associated Gene in Response to Photodynamic Therapy JO - Photodiagnosis and Photodynamic Therapy VL - 18 IS - SP - 78 EP - 82 PY - 2017 ER -