Inductive Role of Sustentacular Cells (Sertoli Cells) Conditioned Medium on Bone Marrow Derived Mesenchymal Stem Cells; [Papel Inductivo Del Medio Acondicionado De Celulas Sustentaculares (Celulas De Sertoli) En Las Celulas Madre Mesenquimales Derivadas De La Medula Osea]

Inductive Role of Sustentacular Cells (Sertoli Cells) Conditioned Medium on Bone Marrow Derived Mesenchymal Stem Cells; [Papel Inductivo Del Medio Acondicionado De Celulas Sustentaculares (Celulas De Sertoli) En Las Celulas Madre Mesenquimales Derivadas De La Medula Osea] Publisher

Monfared MH¹ ; Akbari M¹ ; Kashani IR¹ ; Solhjoo S¹ ; Tooli H¹ ; Omidi A² ; Aliakbari F¹ ; Ijaz S¹ ; Mokhtari T¹ ; Rastegar T¹

Show Affiliations

1. Anatomy Department, School of Medicine, Tehran University of Medical Science, Tehran, Iran
2. Anatomy Department, School of Medicine, Tarbiat Modares University, Tehran, Iran

Source: International Journal of Morphology Published:2017

Abstract

Male germ line stem cells are key factors for male spermatogenesis and fertility. Sustentacular cells (Sertoli cells) as somatic cells play a pivotal role in creating essential microenvironment for the self-renewal and differentiation of the male germ line cells. Mesenchymal stem cells are recognized as self-renewing and multipotent cells able to differentiate into multiple cell types. The generation of male germ cells from mesenchymal stem cells may provide a therapeutic method to treat male infertility. In this study, Bone marrow derived mesenchymal cells (BMMSCs) were retrieved from the bone marrow of 6-8-week old Naval Medical Research Institute (NMRI) mice. Sustentacular cells (Sertoli cells) were isolated and made rich using lectin coated plates. Sustentacular cell condition medium (SCCM) was collected after different time intervals. Then the BMMSCs were cultured with different concentration of SCCM and Dulbecco’s Modified Eagle’s medium (DMEM) at various times. Specific markers of Germ line cells were evaluated by using Reverse transcriptase polymerase chain reaction (RT-PCR) and immunocytochemistry. The results showed that BMMSCs cultured with SCCM for 48h exhibited germ line specific transcripts (Mvh, Iid4, piwil2) (p< 0.05) and markers (Mvh, Scp3). Our findings represent that culturing BMMSCs with SCCM may lead to effective differentiation of BMMSCs into germline cells and provide a treatment strategy for male infertility. © 2017, Universidad de la Frontera. All rights reserved.

Related Docs

View other Related Docs

1. Sertoli Cell Condition Medium Can Induce Germ Like Cells From Bone Marrow Derived Mesenchymal Stem Cells, Iranian Journal of Basic Medical Sciences (2016)

2. Amniotic Membrane Mesenchymal Stem Cells Can Differentiate Into Germ Cells in Vitro, In Vitro Cellular and Developmental Biology - Animal (2016)

3. Multipotent Stem Cell and Current Application, Acta Medica Iranica (2017)

4. Differentiation of Bone Marrow-Derived Stage-Specific Embryonic Antigen 1 Positive Pluripotent Stem Cells Into Male Germ Cells, Microscopy Research and Technique (2017)

5. The Interaction Between Sertoli Cells and Luekemia Inhibitory Factor on the Propagation and Differentiation of Spermatogonial Stem Cells in Vitro, International Journal of Reproductive BioMedicine (2015)

6. In Vitro Differentiation Process of Human Wharton’S Jelly Mesenchymal Stem Cells to Male Germ Cells in the Presence of Gonadal and Non-Gonadal Conditioned Media With Retinoic Acid, In Vitro Cellular and Developmental Biology - Animal (2015)

7. Derivation of Male Germ Cells From Ram Bone Marrow Mesenchymal Stem Cells by Three Different Methods and Evaluation of Their Fate After Transplantation Into the Testis, In Vitro Cellular and Developmental Biology - Animal (2016)

8. Comparison of the Efficacy of Bone Morphogenetic Protein-4 on in Vitro Differentiation of Murine Adipose and Bone Marrow Mesenchymal Stem Cells Into Primordial Germ Cells, Research in Pharmaceutical Sciences (2022)

Experts (# of related papers)

View all Related Experts

Mehdi Abbasi (8)

Fardin Amidi (6)

Style	Citing Format
MLA	Monfared MH, et al.. "Inductive Role of Sustentacular Cells (Sertoli Cells) Conditioned Medium on Bone Marrow Derived Mesenchymal Stem Cells; [Papel Inductivo Del Medio Acondicionado De Celulas Sustentaculares (Celulas De Sertoli) En Las Celulas Madre Mesenquimales Derivadas De La Medula Osea]." International Journal of Morphology, vol. 35, no. 4, 2017, pp. 1597-1606.
APA	Monfared MH, Akbari M, Kashani IR, Solhjoo S, Tooli H, Omidi A, Aliakbari F, Ijaz S, Mokhtari T, Rastegar T (2017). Inductive Role of Sustentacular Cells (Sertoli Cells) Conditioned Medium on Bone Marrow Derived Mesenchymal Stem Cells; [Papel Inductivo Del Medio Acondicionado De Celulas Sustentaculares (Celulas De Sertoli) En Las Celulas Madre Mesenquimales Derivadas De La Medula Osea]. International Journal of Morphology, 35(4), 1597-1606.
Chicago	Monfared MH, Akbari M, Kashani IR, Solhjoo S, Tooli H, Omidi A, Aliakbari F, Ijaz S, Mokhtari T, Rastegar T. "Inductive Role of Sustentacular Cells (Sertoli Cells) Conditioned Medium on Bone Marrow Derived Mesenchymal Stem Cells; [Papel Inductivo Del Medio Acondicionado De Celulas Sustentaculares (Celulas De Sertoli) En Las Celulas Madre Mesenquimales Derivadas De La Medula Osea]." International Journal of Morphology 35, no. 4 (2017): 1597-1606.
Harvard	Monfared MH et al. (2017) 'Inductive Role of Sustentacular Cells (Sertoli Cells) Conditioned Medium on Bone Marrow Derived Mesenchymal Stem Cells; [Papel Inductivo Del Medio Acondicionado De Celulas Sustentaculares (Celulas De Sertoli) En Las Celulas Madre Mesenquimales Derivadas De La Medula Osea]', International Journal of Morphology, 35(4), pp. 1597-1606.
Vancouver	Monfared MH, Akbari M, Kashani IR, Solhjoo S, Tooli H, Omidi A, et al.. Inductive Role of Sustentacular Cells (Sertoli Cells) Conditioned Medium on Bone Marrow Derived Mesenchymal Stem Cells; [Papel Inductivo Del Medio Acondicionado De Celulas Sustentaculares (Celulas De Sertoli) En Las Celulas Madre Mesenquimales Derivadas De La Medula Osea]. International Journal of Morphology. 2017;35(4):1597-1606.
BibTex	@article{ author = {Monfared MH and Akbari M and Kashani IR and Solhjoo S and Tooli H and Omidi A and Aliakbari F and Ijaz S and Mokhtari T and Rastegar T}, title = {Inductive Role of Sustentacular Cells (Sertoli Cells) Conditioned Medium on Bone Marrow Derived Mesenchymal Stem Cells; [Papel Inductivo Del Medio Acondicionado De Celulas Sustentaculares (Celulas De Sertoli) En Las Celulas Madre Mesenquimales Derivadas De La Medula Osea]}, journal = {International Journal of Morphology}, volume = {35}, number = {4}, pages = {1597-1606}, year = {2017} }
RIS	TY - JOUR AU - Monfared MH AU - Akbari M AU - Kashani IR AU - Solhjoo S AU - Tooli H AU - Omidi A AU - Aliakbari F AU - Ijaz S AU - Mokhtari T AU - Rastegar T TI - Inductive Role of Sustentacular Cells (Sertoli Cells) Conditioned Medium on Bone Marrow Derived Mesenchymal Stem Cells; [Papel Inductivo Del Medio Acondicionado De Celulas Sustentaculares (Celulas De Sertoli) En Las Celulas Madre Mesenquimales Derivadas De La Medula Osea] JO - International Journal of Morphology VL - 35 IS - 4 SP - 1597 EP - 1606 PY - 2017 ER -

Style

Citing Format

MLA

Monfared MH, et al.. "Inductive Role of Sustentacular Cells (Sertoli Cells) Conditioned Medium on Bone Marrow Derived Mesenchymal Stem Cells; [Papel Inductivo Del Medio Acondicionado De Celulas Sustentaculares (Celulas De Sertoli) En Las Celulas Madre Mesenquimales Derivadas De La Medula Osea]." International Journal of Morphology, vol. 35, no. 4, 2017, pp. 1597-1606.

APA

Monfared MH, Akbari M, Kashani IR, Solhjoo S, Tooli H, Omidi A, Aliakbari F, Ijaz S, Mokhtari T, Rastegar T (2017). Inductive Role of Sustentacular Cells (Sertoli Cells) Conditioned Medium on Bone Marrow Derived Mesenchymal Stem Cells; [Papel Inductivo Del Medio Acondicionado De Celulas Sustentaculares (Celulas De Sertoli) En Las Celulas Madre Mesenquimales Derivadas De La Medula Osea]. International Journal of Morphology, 35(4), 1597-1606.

Chicago

Monfared MH, Akbari M, Kashani IR, Solhjoo S, Tooli H, Omidi A, Aliakbari F, Ijaz S, Mokhtari T, Rastegar T. "Inductive Role of Sustentacular Cells (Sertoli Cells) Conditioned Medium on Bone Marrow Derived Mesenchymal Stem Cells; [Papel Inductivo Del Medio Acondicionado De Celulas Sustentaculares (Celulas De Sertoli) En Las Celulas Madre Mesenquimales Derivadas De La Medula Osea]." International Journal of Morphology 35, no. 4 (2017): 1597-1606.

Harvard

Monfared MH et al. (2017) 'Inductive Role of Sustentacular Cells (Sertoli Cells) Conditioned Medium on Bone Marrow Derived Mesenchymal Stem Cells; [Papel Inductivo Del Medio Acondicionado De Celulas Sustentaculares (Celulas De Sertoli) En Las Celulas Madre Mesenquimales Derivadas De La Medula Osea]', International Journal of Morphology, 35(4), pp. 1597-1606.

Vancouver

Monfared MH, Akbari M, Kashani IR, Solhjoo S, Tooli H, Omidi A, et al.. Inductive Role of Sustentacular Cells (Sertoli Cells) Conditioned Medium on Bone Marrow Derived Mesenchymal Stem Cells; [Papel Inductivo Del Medio Acondicionado De Celulas Sustentaculares (Celulas De Sertoli) En Las Celulas Madre Mesenquimales Derivadas De La Medula Osea]. International Journal of Morphology. 2017;35(4):1597-1606.

BibTex

@article{ author = {Monfared MH and Akbari M and Kashani IR and Solhjoo S and Tooli H and Omidi A and Aliakbari F and Ijaz S and Mokhtari T and Rastegar T}, title = {Inductive Role of Sustentacular Cells (Sertoli Cells) Conditioned Medium on Bone Marrow Derived Mesenchymal Stem Cells; [Papel Inductivo Del Medio Acondicionado De Celulas Sustentaculares (Celulas De Sertoli) En Las Celulas Madre Mesenquimales Derivadas De La Medula Osea]}, journal = {International Journal of Morphology}, volume = {35}, number = {4}, pages = {1597-1606}, year = {2017} }

RIS

TY - JOUR
AU - Monfared MH
AU - Akbari M
AU - Kashani IR
AU - Solhjoo S
AU - Tooli H
AU - Omidi A
AU - Aliakbari F
AU - Ijaz S
AU - Mokhtari T
AU - Rastegar T
TI - Inductive Role of Sustentacular Cells (Sertoli Cells) Conditioned Medium on Bone Marrow Derived Mesenchymal Stem Cells; [Papel Inductivo Del Medio Acondicionado De Celulas Sustentaculares (Celulas De Sertoli) En Las Celulas Madre Mesenquimales Derivadas De La Medula Osea]
JO - International Journal of Morphology
VL - 35
IS - 4
SP - 1597
EP - 1606
PY - 2017
ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract