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Differential Effects of N-Tio2 Nanoparticle and Its Photo-Activated Form on Autophagy and Necroptosis in Human Melanoma A375 Cells Publisher Pubmed



Mohammadalipour Z1 ; Rahmati M2 ; Khataee A3, 4 ; Moosavi MA1
Authors
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Authors Affiliations
  1. 1. Department of Molecular Medicine, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
  2. 2. Cancer Biology Research Center, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran
  4. 4. Department of Materials Science and Nanotechnology Engineering, Faculty of Engineering, Near East University, Nicosia, North Cyprus, Turkey

Source: Journal of Cellular Physiology Published:2020


Abstract

The manipulation of autophagy provides a new opportunity for highly effective anticancer therapies. Recently, we showed that photodynamic therapy (PDT) with nitrogen-doped titanium dioxide (N-TiO2) nanoparticles (NPs) could promote the reactive oxygen species (ROS)-dependent autophagy in leukemia cells. However, the differential autophagic effects of N-TiO2 NPs in the dark and light conditions and the potential of N-TiO2-based PDT for the treatment of melanoma cells remain unknown. Here we show that depending on the visible-light condition, the autophagic response of human melanoma A375 cells to N-TiO2 NPs switches between two different statuses (ie., flux or blockade) with the opposite outcomes (ie., survival or death). Mechanistically, low doses of N-TiO2 NPs (1-100 µg/ml) stimulate a nontoxic autophagy flux response in A375 cells, whereas their photo-activation leads to the impairment of the autophagosome-lysosome fusion, the blockade of autophagy flux and consequently the induction of RIPK1-mediated necroptosis via ROS production. These results confirm that photo-controllable autophagic effects of N-TiO2 NPs can be utilized for the treatment of cancer, particularly melanoma. © 2020 Wiley Periodicals, Inc.
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