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Measurement of Some Amino Acid Using Biosensors Based on Silicon-Based Carbon Nanotubes Publisher



Eissazadeh S1 ; Piryaei M3 ; Mostafavi SM2
Authors

Source: Journal of Computational and Theoretical Nanoscience Published:2019


Abstract

A sensitive glutamate biosensor based on glutamate dehydrogenase/vertically aligned carbon nanotubes (VACNTs) was synthesized. Carbon nanotubes (CNTs) were grown on a silicon substrate by implementing of Plasma-enhanced chemical vapor deposition method. Glutamate dehydrogenase was fixed covalently on the tip of CNTs. The efficiency of the biosensor was investigated by using cyclic and differential plus voltammetry methods (CV and DPV). The calibration curve of concentration was drawn in the broad range of a 0.1–500 M. The mediator-less biosensor has a low detection limit of 57 nM, and its sensitivity in the range of 0.1–2 M and 20–300 M are 0.976 mA/mM · cm2 and 0.182 mA/mM · cm2, respectively. The impact of other biomaterials on the voltammetric behavior of biosensor was investigated, as well as their response stability. The results were demonstrated that this biosensor even without of utilizing a high sensitive mediator be able to measure glutamate. Copyright © 2019 American Scientific Publishers. All rights reserved.
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