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Cardioprotective Effects of Omega-3 Fatty Acids and Ascorbic Acid Improve Regenerative Capacity of Embryonic Stem Cell-Derived Cardiac Lineage Cells Publisher Pubmed



Shabani P1, 2 ; Ghazizadeh Z1 ; Gorganifiruzjaee S3 ; Molazem M4 ; Rajabi S1 ; Vahdat S1, 5 ; Azizi Y6 ; Doosti M7 ; Aghdami N1 ; Baharvand H1, 8
Authors
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Authors Affiliations
  1. 1. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
  2. 2. Department of Biochemistry, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran
  3. 3. Department of Laboratory Sciences, Faculty of Para Medicine, AJA University of Medical Sciences, Tehran, Iran
  4. 4. Department of Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  5. 5. Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
  6. 6. Physiology Research Center, Department of Physiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  8. 8. Department of Developmental Biology, University of Science and Culture, Tehran, Iran

Source: BioFactors Published:2019


Abstract

One of the major issues in cell therapy of myocardial infarction (MI) is early death of engrafted cells in a harsh oxidative stress environment, which limits the potential therapeutic utility of this strategy in the clinical setting. Increasing evidence implicates beneficial effects of omega-3 fatty acids including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and ascorbic acid (AA) in cardiovascular diseases, in particular their role in ameliorating fibrosis. In the current study, we aim to assess the cytoprotective role of EPA + DHA and AA in protecting embryonic stem cell (ESC)-derived cardiac lineage cells and amelioration of fibrosis. Herein, we have shown that preincubation of the cells with EPA + DHA + AA prior to H2O2 treatment attenuated generation of reactive oxygen species (ROS) and enhanced cell viability. Gene expression analysis revealed that preincubation with EPA + DHA + AA followed by H2O2 treatment, upregulated heme oxygenase-1 (HO-1) along with cardiac markers (GATA4, myosin heavy chain, α isoform [MYH6]), connexin 43 [CX43]) and attenuated oxidative stress-induced upregulation of fibroblast markers (vimentin and collagen type 1 [Col1]). Alterations in gene expression patterns were followed by marked elevation of cardiac troponin (TNNT2) positive cells and reduced numbers of vimentin positive cells. An injection of EPA + DHA + AA-pretreated ESC-derived cardiac lineage cells into the ischemic myocardium of a rat model of MI significantly reduced fibrosis compared to the vehicle group. This study provided evidence that EPA + DHA + AA may be an appropriate preincubation regimen for regenerative purposes. © 2019 BioFactors, 45(3):427–438, 2019. © 2019 International Union of Biochemistry and Molecular Biology
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