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The Effect of Mir-200C Inhibition on Chemosensitivity (5- Fluorouracil) in Colorectal Cancer Publisher Pubmed

Summary: Research suggests blocking miR-200c reduces colon cancer drug resistance, potentially improving treatment outcomes. #CancerResearch #Chemotherapy

Heydari K1 ; Saidijam M1 ; Sharifi M2 ; Dermani FK1 ; Soleimani Asl S3, 4 ; Shabab N1 ; Najafi R1, 4
Authors

Source: Pathology and Oncology Research Published:2018


Abstract

5-Fluorouracil (5-FU) as a chemotherapeutic drug is used to treat colorectal cancer (CRC). However, 5-FU is associated with acquired CRC resistance, which decreases the therapeutic potential of 5-FU. Several studies indicated that miR-200c is also involved in chemotherapeutic drug resistance, but the exact mechanism of miR-200c mediated chemoresistance has not yet been fully understood. In this study, we examined the effect of inhibition of miR-200c on the sensitivity of HCT-116 cells to 5-FU. HCT-116 cells were transfected with LNA-anti- miR-200c for 48 h. mRNA expression of miR-200c was investigated by qRT-PCR. The protein expression of phosphatase and tensin homolog (PTEN) and E-cadherin were evaluated by western blotting. Annexin V/ PI staining and caspase 3 activity were used to detect apoptosis. LNA-anti-miR-200c inhibited the miR-200c expression in the transfected cells compared with that in the control group. LNA-anti-miR-200c suppressed the expression of PTEN and E-cadherin independent of the presence of the chemotherapeutic drug 5-FU. LNA-anti-miR-200c reduced the 5-FU-induced apoptosis and caspase 3 activity. miR-200c, as a novel prognostic marker in CRC, can be a potential therapeutic approach to overcome chemoresistance during 5-FU chemotherapy. © 2017, Aranyi Lajos Foundation.
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