Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
How Effective Are Alprostadil and Hydrocortisone on Reperfusion Injury in Kidney After Distant Organ Ischemia?



Ebrahimi A1 ; Salimi F2 ; Safaei M2 ; Melali H2 ; Jazi AHD1, 2 ; Nematbakhsh M3 ; Mokhtari M4 ; Rasooli H1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Departments of Plastic Surgery, Trauma Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
  2. 2. Departments of Plastic Surgery, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Physiology/ Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Pathology, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Research in Medical Sciences Published:2013

Abstract

Background: After reestablishment of blood flow to ischemic limb recirculation of free radicals may cause ischemia-reperfusion injury in many organs. This study designed to investigate effects of hydrocortisone and alprostadil distant injury to kidneys by both measuring biochemical markers of oxidative stress and histopathologic examination in an experimental rat model of hind limb ischemia-reperfusion. Materials and Methods: This study conducted in Isfahan University of Medical Sciences during 2011-2012. Ischemia was established by infra renal aortic clamping for 60 min in 32 male Wistar rats. Animals were divided into those receiving alprostadil (group ischemia-reperfusion plus alprostadil (IR/A), n = 8), those receiving hydrocortisone (group ischemia-reperfusion plus hydrocortisone (IR/H), n = 8), control group (group ischemia-reperfusion (IR), n = 8), and sham group (n = 8). After 120 min of reperfusion both kidneys were removed. Levels of superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione (GSH) as indirect markers of oxidative injury was measured. Finally all data in different groups were compared using the analysis of variance (ANOVA) test by Statistical Package for Social Sciences (SPSS) version 16. Results: Administration of alprostadil or hydrocortisone does not improve the biochemical parameters of oxidative injury including MDA and SOD. However, statistically significant difference was seen in GSH level among sham and IR groups. Mean (± standard deviation (SD)) concentration of GSH in IR, IR/A, IR/H, and sham groups were 1028.77 (72.65), 924.82 (70.66), 1000.28 (108.77), and 846.69 (163.52), respectively (P = 0.015). Histopathological study of specimens did not show any significant changes between groups. Conclusion: Alprostadil and hydrocortisone do not improve the kidney GSH, SOD, and MDA level and kidney releases its GSH reserve during ischemia-reperfusion event, and another point is that, 3 h of ischemia-reperfusion does not develop injury in kidney.
Related Docs
View other Related Docs
1. Cardiopulmonary Protective Effects of Alprostadil and Hydrocortisone Against Distant Organ Reperfusion After Hind Limb Ischemia in Rat Model, Italian Journal of Vascular and Endovascular Surgery (2015)
2. Sodium Hydrogen Sulfide May Not Protect the Kidney Against Ischemia/Reperfusion Damage in Male and Female Rats, Research in Pharmaceutical Sciences (2023)
3. Evaluating the Effect of Remote Ischemic Preconditioning on Kidney Ischemia-Reperfusion Injury, Journal of Research in Medical Sciences (2020)
4. The Protective Roles of Zinc and Estradiol in Renal Ischemia/Reperfusion Injury in Ovariectomized Rats, Journal of Nephropathology (2018)
5. Mas Receptor Blockade Promotes Renal Vascular Response to Ang Ii After Partial Kidney Ischemia/Reperfusion in a Two-Kidney-One-Clip Hypertensive Rats Model, International Journal of Nephrology (2021)
6. The Effect of At2 and Mas Receptors Antagonists on Renal Hemodynamic and Excretory Disorders Induced by Ischemia/Reperfusion in Male and Female Rats, Physiology and Pharmacology (Iran) (2018)
7. Naloxone Pretreatment Prevents Kidney Injury After Liver Ischemia Reperfusion Injury, International Urology and Nephrology (2016)
8. Zinc Supplementation and Ischemia Pre-Conditioning in Renal Ischemia/ Reperfusion Injury, Malaysian Journal of Medical Sciences (2019)
Experts (# of related papers)
View all Related Experts
Mehdi Nematbakhsh (25)
Ardeshir Talebi (10)
Other Related Docs
9. Pretreatment Effect of D-Limonene on Oxidative Stress Induced by Renal Ischemia-Reperfusion Injury in Rats, Journal of Babol University of Medical Sciences (2022)
10. N-Acetylcysteine Prevents Kidney and Lung Disturbances in Renal Ischemia/ Reperfusion Injury in Rat, International Journal of Preventive Medicine (2013)
11. Testosterone and Zinc Supplementations on Renal Ischemia-Reperfusion Injury in Orchiectomized Rats, International Journal of Preventive Medicine (2019)
12. Protective Effect of N-Acetylcysteine on Kidney As a Remote Organ After Skeletal Muscle Ischemia-Reperfusionspi; [Efeito Protetor Da N-Acetilcisteina No Rim Como Um Orgao Remoto Musculo Esqueletico Apos Isquemia-Reperfusao], Acta Cirurgica Brasileira (2012)
13. Renal Vascular Responses to Angiotensin Ii Infusion in Two Kidneys-One Clip Hypertensive Rats Under Partial Ischemia/Reperfusion With and Without Ischemia Preconditioning: The Roles of At1r Blockade and Co-Blockades of At1r and Masr, Research in Pharmaceutical Sciences (2023)
14. Preventive Role of Endothelin Antagonist on Kidney Ischemia: Reperfusion Injury in Male and Female Rats, International Journal of Preventive Medicine (2015)
15. Tramadol Alleviates Myocardial Injury Induced by Acute Hindlimb Ischemia Reperfusion in Rats; [Tramadol Atenua Lesoes Miocardicas Induzidas Pela Reperfusao Pos- Isquemica Aguda De Membros Posteriores Em Ratos], Arquivos Brasileiros de Cardiologia (2015)
16. Effects and Mechanisms of Fisetin Against Ischemia-Reperfusion Injuries: A Systematic Review, Current Pharmaceutical Biotechnology (2024)
17. Protective Role of Recombinant Human Erythropoietin in Kidney and Lung Injury Following Renal Bilateral Ischemia-Reperfusion in Rat Model, International Journal of Preventive Medicine (2013)
18. The Effects of Diethyldithiocarbamate, a Sod Inhibitor, on Endothelial Function in Sedentary and Exercised Db/Db Mice, Pathophysiology (2009)
19. Effects of Hypertension on Hemodynamic Response and Serum Nitrite Concentration During Graded Hemorrhagic Shock in Rats, Journal of Research in Medical Sciences (2011)
20. Effect of Tramadol on Lung Injury Induced by Skeletal Muscle Ischemia-Reperfusion: An Experimental Study; [Efeito Do Tramadol Na Lesao Pulmonar Induzida Por Isquemia-Reperfusao De Musculo Esqueletico: Um Estudo Experimental], Jornal Brasileiro de Pneumologia (2013)
21. The Sesame Lignan Sesamin Attenuates Vascular Permeability in Rats With Streptozotocin-Induced Diabetes: Involvement of Oxidative Stress, International Journal of Endocrinology and Metabolism (2011)
22. Gender Difference in Renal Blood Flow Response to Angiotensin Ii Administration After Ischemia/Reperfusion in Rats: The Role of At2 Receptor, Advances in Pharmacological Sciences (2016)
23. Time Course of Renal Ischemia/Reperfusion and Distance Organ: Lung Dysfunction in Male and Female Rats, Physiology and Pharmacology (Iran) (2022)
24. Effects of Light-Emitting Diode (Led) Therapy on Skeletal Muscle Ischemia Reperfusion in Rats, Lasers in Medical Science (2015)
25. Mas Receptor Antagonist (A799) Alters the Renal Hemodynamics Responses to Angiotensin Ii Administration After Renal Moderate Ischemia/Reperfusion in Rats: Gender Related Differences, Research in Pharmaceutical Sciences (2019)
26. Local Maximum Oxygen Disappearance Rate Has Limited Utility As a Measure of Local Renal Tissue Oxygen Consumption, Journal of Pharmacological and Toxicological Methods (2010)
27. The Protective Effect of Γ‑Aminobutyric Acid on Kidney Injury Induced by Renal Ischemia‑Reperfusion in Ovariectomized Estradiol‑Treated Rats, International Journal of Preventive Medicine (2016)
28. Evaluating the Effect of Mebudipine on Hemodynamical Parameters and Histological Changes in Isolated Heart Induced by Ischemic-Reperfusion Injury in Male Rat, Pharmaceutical Sciences (2009)
29. Hemodynamic Responses and Serum Nitrite Concentration During Uncontrolled Hemorrhagic Shock in Normotensive and Hypertensive Rats, Biomedical Papers (2012)
30. Protective Effect of Gastric Distension Preconditioning on Renal Ischemia/Reperfusion Injury in Rats, Indian Journal of Nephrology (2018)
31. Protective Role of Angiotensin Ii Type 1 Receptor Blocker on Short Time Effect of Oleic Acid Induced Lung and Kidney Injury, International Journal of Preventive Medicine (2021)
32. The Effect of the Various Doses of Atorvastatin on Renal Tubular Cells; an Experimental Study, Journal of Nephropathology (2016)
33. The Reno-Protective Role of Angiotensin 1-7 in Cisplatin Induced Nephrotoxicity, Kuwait Medical Journal (2020)
34. The Histobiochemical Effects of Melatonin on Ischemia Reperfusion-Related Injuries in Vascular Trauma of Lower Limbs, Annali Italiani di Chirurgia (2012)
35. Preventive Role of Estradiol on Kidney Injury Induced by Renal Ischemia-Reperfusion in Male and Female Rats, International Journal of Preventive Medicine (2015)
36. Multiple Mechanisms Act to Maintain Kidney Oxygenation During Renal Ischemia in Anesthetized Rabbits, American Journal of Physiology - Renal Physiology (2010)
37. Astaxanthin Prevents Nephrotoxicity Through Nrf2/Ho-1 Pathway, Canadian Journal of Physiology and Pharmacology (2024)
38. The Role of the At1 Receptor Antagonist on Renal Hemodynamic Responses to Angiotensin 1-7 in Acute Sympathectomized Male and Female Rats, Physiology and Pharmacology (Iran) (2024)
39. The Effect of 2,3-Dihydroxybenzoic Acid and Tempol in Prevention of Vancomycin-Induced Nephrotoxicity in Rats, Toxicology (2007)
40. L-Carnitine and Potential Protective Effects Against Ischemia-Reperfusion Injury in Noncardiac Organs: From Experimental Data to Potential Clinical Applications, Journal of Dietary Supplements (2018)
41. Antihypertensive and Antioxidant Effects of Protocatechuic Acid in Deoxycorticosterone Acetate-Salt Hypertensive Rats, Biomedicine and Pharmacotherapy (2018)
42. Nitric Oxide Metabolite Changes in Gentamicin-Induced Nephrotoxicity; the Effects of Antioxidant Vitamins, Journal of Renal Injury Prevention (2018)
43. Protection of Renal Tubular Cells by Antioxidants: Current Knowledge and New Trends, Cell Journal (2015)
44. Expression of Bcl-2 and Bax After Hippocampal Ischemia in Dha + Epa Treated Rats, Neurological Sciences (2011)
45. Protective Effects of N-Acetylcysteine on Myocardial Injury Induced by Hindlimb Ischaemia–Reperfusion: A Histological Study in a Rat Model, Comparative Clinical Pathology (2014)
46. Paradoxical Effects of Atorvastatin on Renal Tubular Cells an Experimental Investigation, Iranian Journal of Kidney Diseases (2015)
47. View of the Renin-Angiotensin System in Acute Kidney Injury Induced by Renal Ischemia-Reperfusion Injury, JRAAS - Journal of the Renin-Angiotensin-Aldosterone System (2022)
48. Beneficial Effects of Dapsone on Ischemia/Reperfusion Injury Following Torsion/Detorsion in Ipsilateral and Contralateral Testes in Rat, Theriogenology (2019)