Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Proanthocyanidin As a Crosslinking Agent for Fibrin, Collagen Hydrogels and Their Composites With Decellularized Wharton’S-Jelly-Extract for Tissue Engineering Applications Publisher



Hasanzadeh E1 ; Mahmoodi N2 ; Basiri A3 ; Esmaeili Ranjbar F4, 5 ; Hassannejad Z6 ; Ebrahimibarough S4 ; Azami M4 ; Ai J4 ; Rahimimovaghar V2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  2. 2. Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Biomaterials and Tissue Engineering, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Faculty of Allied Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran
  6. 6. Pediatric Urology and Regenerative Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Bioactive and Compatible Polymers Published:2020


Abstract

In tissue engineering, natural hydrogel scaffolds gained considerable attention due to their biocompatibility and similarity to macromolecular-based components in the body. However, their low mechanical strength and high degradation degree limit their biomedical application. By varying the composition of hydrogels, their biochemical and mechanical properties can be improved. In this study, the stability of fibrin and collagen hydrogels and their composites with decellularized Wharton’s jelly extract (DEWJ) was improved using proanthocyanidin (PA) as a cross-linker, extracted from grape seeds. The cytocompatibility, physicochemical and mechanical properties of the hydrogels were evaluated. Human endometrial stem cells (hEnSCs) were seeded on the hydrogels and their attachment, morphology, and proliferation were investigated using a scanning electron and optical microscopy. Our results showed that hydrogels containing DEWJ along with PA enhance cell proliferation and showed higher mechanical properties compared with the fibrin and collagen hydrogel. The results present the potential utility of these hydrogels in tissue engineering and for application in three-dimensional culture. © The Author(s) 2020.
Other Related Docs
12. Effects of Nanozeolite/Starch Thermoplastic Hydrogels on Wound Healing, Journal of Research in Medical Sciences (2017)
33. Electrospun Polycaprolactone/Gelatin/Bioactive Glass Nanoscaffold for Bone Tissue Engineering, International Journal of Polymeric Materials and Polymeric Biomaterials (2019)
47. Nanofibrous Scaffolds With Biomimetic Composition for Skin Regeneration, Applied Biochemistry and Biotechnology (2019)