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Calcitonin-Loaded Octamaleimic Acid–Silsesquioxane Nanoparticles in Hydrogel Scaffold Support Osteoinductivity in Bone Regeneration Publisher Pubmed



Ahmadipour S1, 2 ; Varshosaz J1 ; Hashemibeni B3 ; Safaeian L4 ; Manshaei M5 ; Sarmadi A6
Authors
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Authors Affiliations
  1. 1. Department of Pharmaceutics, School of Pharmacy and Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Pharmaceutics, School of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran
  3. 3. Department of Anatomical Sciences, Faculty of Medicine, Torabinejad Dental Research Center, Dental School, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Pharmacology and Toxicology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
  5. 5. Dental Research Center, Dental Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran
  6. 6. Cellular and Molecular Research Center, Basic Health Sciences Institute, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Pharmaceutical Development and Technology Published:2021


Abstract

Novel osteoinductive scaffolds fabricated using the benefits of tissue engineering techniques accompanied by utilizing drugs can accelerate bone regeneration. The purpose of this study was to load salmon calcitonin (sCT) in octamaleimic acid-silsesquioxane (OMA-POSS) nanoparticles and enrich the hydrogel scaffold based on hydroxyapatite, Gelrite® and platelet-rich plasma (PRP) for use in bone tissue engineering. The loading efficiency, release percentage, particle size and zeta potential of the nanoparticles were evaluated. The proliferation of seeded MG-63 osteoblast cells on the designed scaffold, its cytotoxicity and osteo-conductivity were studied by alkaline phosphatase measurement and Alizarin red staining. The expression of cellular osteogenic markers such as collagen 1 (COL1A1), osteocalcin (BGLAP) and osteopontin (SPP1) was examined using reverse transcription polymerase chain reaction. The results revealed that the particle size of the nanoparticles varied between 94.2 and 199.2 nm and their negative surface charge increased after drug conjugation. The osteoblast cell proliferation and calcium granule production in the optimum formulation were significantly higher in comparison with the control group (p < 0.05). Osteogenic markers increased significantly after a specific number of days of cell culture compared to the control group (p < 0.05). The results also showed the potential of the designed scaffold in bone tissue engineering. © 2020 Informa UK Limited, trading as Taylor & Francis Group.
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