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Effect of Peg Molecular Weight and Volume Ratio of Chitosan/Peg and Silk Fibroin on Physicomechanical Properties of Chitosan/Peg-Sf Scaffold As a Bio-Mimetic Substrate in Skin-Tissue Engineering Applications Publisher



Aghmiuni AI1 ; Keshel SH2, 3 ; Rezaeitavirani M4 ; Sefat F5, 6 ; Khojasteh A3, 7 ; Soleimani M2, 3 ; Pakdel F8
Authors
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Authors Affiliations
  1. 1. Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran, 1316943551, Iran
  2. 2. Medical Nanotechnology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, 1985711151, Iran
  3. 3. Department of Tissue Engineering and Applied Cell Science, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, 19839-63113, Iran
  4. 4. Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, 19839-63113, Iran
  5. 5. Department of Biomedical and Electronics Engineering, School of Engineering, University of Bradford, Bradford, BD7 IDP, United Kingdom
  6. 6. Interdisciplinary Research Centre in Polymer Science & Technology, University of Bradford, Bradford, BD7 IDP, United Kingdom
  7. 7. Dental Research Center, Research Institute of Dental Sciences, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, 19839-63113, Iran
  8. 8. Department of Oculo-Facial Plastic Surgery, Farabi Hospital, Tehran University of Medical Sciences, Tehran, 1336616351, Iran

Source: Fibers and Polymers Published:2022


Abstract

Tissue engineering provides new approaches to improve skin lesions. However, cell differentiation onto the engineered substrate with the skin-like pattern is the main challenge. Here we have tried to fabricate such the substrate via studying the change in polymers ratios and molecular weight, and grafting scaffold with silk fibroin (SF) biomaterial. To this end, chitosan and PEG were mixed at the volume ratios of 25:75, 50:50, and 65:35, and samples were lyophilized by the freeze-drying method. Based on the result, the ratio of 65:35 indicated better physicomechanical properties than two other scaffolds. Afterward, Chi/PEG scaffolds were prepared via mixing chitosan/PEG with (65:35) and PEG molecular weights of 2000, 4000, 6000, 10000 Da. It was found that the increase of PEG molecular weight (>4000) was led to the reduction in tensile strength and elongation of the scaffold network. Hence, PEG4000 was selected as the optimum molecular weight to design SF-grafted Chi/PEG scaffold. Therefore, Chi/PEG4000-SF scaffold was designed to evaluate the volume ratio of SF (1 %, 3 %, 5 %) and compare data with the decellularized dermis. The results showed Chi/PEG4000-SF(3%) scaffold not only was led to the same elongation as Chi/PEG-SF(5%) scaffold but also created the dermis-like modulus. Moreover, Chi/PEG-SF provided higher expression level of keratinocytes (bio-mimetic pattern) than decellularized dermis due to better physicomechanical properties. Hence, it seems that engineered scaffolds can be a more suitable option than native tissue (due to removal of limitations such as donor sites and immunogenicity, and their mechanical properties). This study can provide novel insight into the better design of skin-engineered scaffolds. © 2022, The Korean Fiber Society.