Antimicrobial Effect Based on Activated Persulfate Using Nano-Magnetite Nanozyme Immobilized on the Microbial Cellulose Hydrogel Publisher



Ahmadi S¹ ; Dehghani MH² ; Rezaee A¹ Show Affiliations
Source: Journal of the Taiwan Institute of Chemical Engineers Published:2025

Abstract

Background: The present study is to investigate the antimicrobial activity of the activated persulfate (PS) using the nanocomposite fabricated by microbial cellulose hydrogel (MCH)/nanomagnetite (Fe3O4) nanoparticles (NPs) as a nanozyme. Methods: The main reaction parameters including nanocomposite concentration, persulfate concentration and pH values were investigated. Various analysis, such as dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), field emission-scanning electron microscopy (FE-SEM), and vibrating-sample magnetometry (VSM), were utilized to analyze the nanocomposite and the nanozyme. Significant findings: The obtained results show that the nanocomposite has antimicrobial activity under optimum conditions (temperature: 25 °C, nanozyme concentration: 2 mg/mL, and solution pH: 4.0). Moreover, increasing of the antimicrobial activity of the nanocomposite is observed using 0.25 g/l PS. The nanozyme exhibits the Michaelis constant (Km) of 0.32 mM and the maximum reaction velocity (Vmax) of 0.6 10−8 mM sec−1 for 3,3′,5,5′-tetramethylbenzidine (TMB). The reason for the increasing of the antimicrobial activity is due to the generation of higher active oxygen species (ROS), which improve the elimination of the E. coli. © 2025