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Erythropoietin/Aloe Vera-Releasing Wet-Electrospun Polyvinyl Alcohol/Chitosan Sponge-Like Wound Dressing: In Vitro and in Vivo Studies Publisher



Naserinosar M1 ; Farzamfar S2 ; Salehi M3 ; Vaez A1 ; Tajerian R1 ; Azami M1
Authors
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Authors Affiliations
  1. 1. Department of Tissue Engineering & Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran

Source: Journal of Bioactive and Compatible Polymers Published:2018


Abstract

This study aimed to develop a recombinant human erythropoietin/aloe vera gel–releasing polyvinyl alcohol/chitosan wound dressing by wet-electrospinning method and investigate the synergistic effects of erythropoietin and aloe vera gel on the excisional wound healing. The polyvinyl alcohol/chitosan (4:1 (w/w)) solution was electrospun into an aloe vera gel–containing water coagulation bath and then coated with the recombinant human erythropoietin–loaded chitosan nanoparticles prepared via ionic gelation technique. The developed sponge-like film was able to release the erythropoietin and aloe vera gel for at least 7 and 1 days, respectively. The dressing was non-toxic to L929 fibroblastic cell line and non-adherent to the wound bed with the contact angle of 67.93° ± 2.31°. It also possessed the water-uptake capacity and water vapor transmission rate of 26.40% ± 3.37% and 2584.00 ± 144.67 g m−2, respectively. The in vivo study demonstrated the synergistic wound-healing effects of aloe vera gel and erythropoietin on the full-thickness excisional wounds of Wistar rats. The erythropoietin/aloe vera gel–releasing dressing had significantly (n = 4, p < 0.005) higher wound closure (92.96% ± 10.09%) than the sterile gauze (73.89% ± 2.61%), as the control group, after 2 weeks. The differences observed between the wound closure percentages of the aloe vera gel–releasing dressing (85.93% ± 6.46%) and the erythropoietin/aloe vera gel–releasing dressing and control group were not statistically significant. © 2017, © The Author(s) 2017.
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