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Retinoic Acid-Oligomeric Chitosan Micelles As Novel Gene Delivery Carrier; in Vitro Transfection Study



Fattahi A1, 2, 3 ; Golozar MA3 ; Varshosaz J4
Authors
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Authors Affiliations
  1. 1. Nanosciences and technology research center, Kermanshah University of Medical Sciences, Kermanshah, 67346-67149, Iran
  2. 2. Novel Drug Delivery Research Center, Kermanshah University of Medical Sciences, Kermanshah, 67346-67149, Iran
  3. 3. Department of Materials Engineering, Isfahan University of Technology, Isfahan, 84146-83111, Iran
  4. 4. Department of Pharmaceutics, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, 81746-73461, Iran

Source: Journal of Reports in Pharmaceutical Sciences Published:2013

Abstract

Amphiphilic cationic micelles have recently attracted much attention as non-viral vector. In the current study the retinoic acid-g-chitosan (RA-chitosan) micelles have been used as novel gene carriers. Uptake of micelles, condensation ability and transfection efficiency of micelle–DNA complexes (miceplexes) in the presence or absence of serum had been investigated on Hela and HepG2 cell lines. Uptake of micelles in Hela cells was faster and more effective than HepG2. After 1.5 hours, micelles were up-taken by Hela cells while no uptake was observed in HepG2 cells. Miceplexes showed lower transfection efficiency in both Hela and HepG2 cells grown in the serum free medium than chitosan polyplexes, with 7.25 × 105 versus 1.47 × 105 RLU.mg-1 and 7.76 × 105 versus 2.16 × 105 RLU.mg-1, respectively. Lower transfection efficiency of miceplexes could be attributed to their stronger complexation. Transfection in the presence of serum increased in both of the cell lines. These properties all together, make RA-chitosan micelles a potential gene carrier for active gene delivery into cells, particularly for transfection in the presence of serum. © 2013 by Kermanshah University of Medical Sciences.
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