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Comparing the Transfection Efficiency of Cationic Monomer Ratios in Vinylimidazole and Aminoethyl Methacrylate Copolymers Publisher



Soghrati S1 ; Varshosaz J1 ; Rostami M2 ; Mirian M3 ; Sharifianjazi F4 ; Tavamaishvili K5
Authors
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Authors Affiliations
  1. 1. Novel Drug Delivery Systems Research Centre, Department of Pharmaceutics, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Novel Drug Delivery Systems Research Centre and Department of Medicinal Chemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Natural Sciences, School of Science and Technology, University of Georgia, Tbilisi, Georgia
  5. 5. Georgian American University, School of Medicine, 10 Merab Aleksidze Str, Tbilisi, 0160, Georgia

Source: International Journal of Pharmaceutics: X Published:2025


Abstract

Employing polycations as non-viral gene delivery vectors has been extensively studied owing to their safety, efficiency and possibility of modifying them in an intended way compared with viral vectors. However, the main challenge is finding a biocompatible and transfection-efficient polymer. In this study, 2-aminoethyl methacrylate (A) and 1-vinyl imidazole (V) were copolymerized at three different molar ratios by a free radical polymerization method and novel biocompatible polycations with narrow molecular weight distribution were obtained. The resulting copolymers were used for condensation of plasmid DNA (pDNA) at different N/P ratios followed by physicochemical characterizations of resulting polyplexes. At N/P ratio of 2, the nanoplexes were smaller than 120 nm. The optimum formulations were stable in presence of polyanions and capable of protecting the condensed pDNA against nucleases. The polyplexes having V to A molar ratio of 1:1 were the most efficient carrier in transfecting HeLa cells and were introduced as a promising non-viral vector. © 2025
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