Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Dose-Response Modeling Using Mtt Assay: A Short Review



Nikzad S1 ; Baradaranghahfarokhi M2 ; Nasri P3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Medical Physics, Tarbiat Modares University, Tehran, Iran
  2. 2. Department of Medical Physics, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Life Science Journal Published:2014

Abstract

MTT assay is widely used to determine the response of cells to ionizing radiation. This method has several advantages such as relative low cost, short time to assess the samples and the possibility of acquiring the results semi-automatically. However, for this method, there are some restrictions/limitations and some important factors that should be considered. This paper is focused to discuss a standard procedure in using MTT assay for calculating the cell survival after irradiation.
Related Docs
View other Related Docs
1. Quality of Medical Care in Mammography Divisions: A Report and Review of the Literature, Life Science Journal (2014)
2. Mobile Phone Radiation and Human Serum Components: A Short Literature Review on Recent Findings, Life Science Journal (2014)
3. Cisplatin-Induced Renal Toxicity: A Short Review, Life Science Journal (2014)
4. Impact of Serum Uric Acid on the Aggravation of Renal Function; Current Knowledge and New Concepts, Life Science Journal (2014)
5. Multiple Mts Assay As the Alternative Method to Determine Survival Fraction of the Irradiated Ht-29 Colon Cancer Cells, Journal of Medical Signals and Sensors (2016)
6. Comparison of the Radiosensitivity of Cancer and Normal Cells to X-Ray Irradiation Using Mtt Assay: An In-Vitro Study, Journal of Isfahan Medical School (2018)
7. Photoneutron Shielding Design for an 18 Mv Saturne 20 Medical Linear Accelerator, Journal of Isfahan Medical School (2014)
8. World Kidney Day 2016: Awareness for Saving Kidneys, Shiraz E Medical Journal (2015)
Experts (# of related papers)
View all Related Experts
Mehdi Nematbakhsh (16)
Ardeshir Talebi (10)
Other Related Docs
9. Cisplatin and Herbal Antioxidants: Protective Effect of Pomegranate Seed Oil Against Cisplatin-Induced Nephrotoxicity in Rat, Renal Failure (2016)
10. Comparing the Patterns of Hypertension and Type 2 Diabetes Mellitus in Patients With Colorectal Cancer, Journal of Isfahan Medical School (2014)
11. Serum Lipoprotein (A) in Diabetic Patients With Various Renal Function Not yet on Dialysis, Pakistan Journal of Medical Sciences (2013)
12. Cisplatin-Induced Nephrotoxicity; Protective Supplements and Gender Differences, Asian Pacific Journal of Cancer Prevention (2017)
13. The Effects of Vitamin E and Selenium on Cisplatin-Induced Nephrotoxicity in Cancer Patients Treated With Cisplatin-Based Chemotherapy: A Randomized, Placebo-Controlled Study, Journal of Research in Medical Sciences (2013)
14. Evaluation of Measuring Radiation-Induced Apoptosis in Human T-Lymphocytes by Flow Cytometry As a Biological Dosimetry System, Journal of Isfahan Medical School (2014)
15. The Evaluation of L-Arginine Prophylaxis and Treatment on Preventing Nephrotoxicity Cisplatin-Induced in Rat: Role of Gender Difference, Journal of Isfahan Medical School (2015)
16. The Prevention of Cisplatin-Induced Nephrotoxicity: A General Consensus Statement of a Group of Oncologist-Hematologists, Adult and Pediatric Nephrologists, Radiation Oncologists, Clinical Pathologists, Clinical Pharmacologists, and Renal Physiologists on Cisplatin Therapy in Cancer Patients, International Journal of Preventive Medicine (2022)
17. Comparison of High-Flux and Low-Flux Hemodialysis Filters on Hemodialysis Adequacy in Under-Hemodialysis Patients With End-Stage Renal Disease, Journal of Isfahan Medical School (2015)
18. World Kidney Day 2014: Chronic Kidney Disease and Aging, Journal of Isfahan Medical School (2015)
19. The Protective Effect of Recombinant Human Erythropoietin Against Cisplatin-Induced Renal and Hepatic Dysfunctions in Wistar Rats, Human and Experimental Toxicology (2014)
20. Re: Effect of Alcoholic Extract of Nigella Sativa on Cisplatininduced Toxicity in Rat, Iranian Journal of Kidney Diseases (2013)
21. Remedial Effect of Boswellia Serrata on Thermal Burn Injuries, Shiraz E Medical Journal (2015)
22. Cisplatin Nephrotoxicity May Be Sex Related, Kidney International (2013)
23. A Model for Prediction of Cisplatin Induced Nephrotoxicity by Kidney Weight in Experimental Rats, Journal of Research in Medical Sciences (2013)
24. Sex Differences in Protective Effect of Recombinant Human Erythropoietin Against Cisplatin-Induced Nephrotoxicity in Rats, Iranian Journal of Kidney Diseases (2013)
25. The Effects of Fenugreek on Radiation Induced Toxicity for Human Blood T-Cells in Radiotherapy, Journal of Medical Signals and Sensors (2015)
26. The Role of Folic Acid-Conjugated Polyglycerol Coated Iron Oxide Nanoparticles on Radiosensitivity With Clinical Electron Beam (6 Mev) on Human Cervical Carcinoma Cell Line: In Vitro Study, Journal of Photochemistry and Photobiology B: Biology (2018)
27. Dextrose Hydration May Promote Cisplatin-Induced Nephrotoxicity in Rats: Gender-Related Difference, Indonesian Biomedical Journal (2019)
28. Estimation of Cell Response in Fractionation Radiotherapy Using Different Methods Derived From Linear Quadratic Model, Radiology and Oncology (2015)
29. Comparison of Radiotherapy and Cold Plasma Treatment Approaches in Inhibiting the Growth of Cancer Cells; [مقایسهی روشهای درمانی رادیوتراپی و پلاسمای سرد در مهار رشد سلولهای سرطانی], Journal of Isfahan Medical School (2024)
30. Vitamin E, Vitamin C, or Losartan Is Not Nephroprotectant Against Cisplatin-Induced Nephrotoxicity in Presence of Estrogen in Ovariectomized Rat Model, International Journal of Nephrology (2012)
31. Protective Role of Angiotensin Ii Type 1 Receptor Blocker on Short Time Effect of Oleic Acid Induced Lung and Kidney Injury, International Journal of Preventive Medicine (2021)
32. Evaluation of Absorbed Dose From Common Radiology Examinations in Yasuj, Iran Using Experimental Measurement and Monte Carlo Calculations, Journal of Isfahan Medical School (2016)
33. Irisin, a New Biomarker in Diagnosis of Atherosclerosis and Myocardial Infarction, Journal of Isfahan Medical School (2015)
34. Effect of Temporal Pattern of Radiation in Intensity Modulated Radiotherapy on Cell Cycle Progression and Apoptosis of Achn Renal Cell Carcinoma Cell Line, Pakistan Journal of Biological Sciences (2016)
35. Nonpreventive Role of Aerobic Exercise Against Cisplatin-Induced Nephrotoxicity in Female Rats, International Journal of Preventive Medicine (2015)
36. The Reno-Protective Role of Angiotensin 1-7 in Cisplatin Induced Nephrotoxicity, Kuwait Medical Journal (2020)
37. Response to Nephrotoxicity: Evidence in Patients Receiving Cisplatin Therapy, Clinical Journal of Oncology Nursing (2018)
38. Evaluation of Testicular Dose and Associated Risk From Common Pelvis Radiation Therapy in Iran, Physica Medica (2014)
39. Correlation of Serum Magnesium and Blood Lipid Profile in Under- Peritoneal Dialysis and Hemodialysis Patients, Journal of Isfahan Medical School (2014)
40. A Protocol for Irradiation of Cell Lines Cultured in Multi-Well Plates: Effect of Air Inhomogeneity on Irradiated Cell Survival, International Journal of Radiation Biology (2019)
41. Intravenous Administration of Cisplatin With Magnesium Sulfate Supplement May Prevent Kidney Toxicity in Rats: The Role of Gender and Magnesium Sulfate Dose, International Journal of Nephrology (2022)
42. Association of Serum Uric Acid With Proteinuria in Type 2 Diabetic Patients, Journal of Research in Medical Sciences (2013)
43. Evaluating the Radioprotective Effect of Cimetidine, Imod, and Hybrid Radioprotectors Agents: An In-Vitro Study, Applied Radiation and Isotopes (2021)
44. Evaluating the Effectiveness of Small Fractionation Radiotherapy Regimens on the Achn Renal Cell Carcinoma Cell Line, Journal of Isfahan Medical School (2015)
45. Biochemical and Histopathological Changes in Kidney of Diabetic Rats Treated With Hydroalcoholic Extract of Centaurea Cyanus, Journal of Mazandaran University of Medical Sciences (2016)
46. Effects of Mobile Phone Prolonged Radiation on Kidney Cells; an In-Vitro Study, Journal of Renal Injury Prevention (2018)
47. A Suitably Designed Lip Applicator for Radiation Therapy of Lips Carcinoma, Journal of Isfahan Medical School (2014)
48. The Effects of 900-Mhz Mobile Phone Radiation on Survival and Proliferation Rate of Breast Cancer Mcf-7 Cells: An In-Vitro Study, Journal of Isfahan Medical School (2017)
49. Prevention of Hepatitis B Virus Reactivation in Kidney Transplant Recipients, Journal of Babol University of Medical Sciences (2016)
50. Photoneutron Contamination From an 18 Mv Saturne Medical Linear Accelerator in the Treatment Room, Radiation Protection Dosimetry (2013)