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Detection of Efflux Pump Genes in Multiresistant Acinetobacter Baumannii St2 in Iran Publisher Pubmed



Meshkat Z1 ; Salimizand H2, 3 ; Amini Y4, 5 ; Mansury D6 ; Zomorodi AR7 ; Avestan Z8 ; Jamee A9 ; Falahi J10 ; Farsiani H1 ; Mojahed A11
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Authors Affiliations
  1. 1. Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
  2. 2. Liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
  3. 3. Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
  4. 4. Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran
  5. 5. Department of Microbiology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
  6. 6. Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  7. 7. Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi university of Mashhad, Mashhad, Iran
  8. 8. Emam Khomeini Hospital of Naqadeh, Urmia University of Medical Sciences, Urmia, Iran
  9. 9. Department of Endodontics, Dental Faculty, Urmia University of Medical Sciences, Urmia, Iran
  10. 10. Department of Medicine, Zahedan Medical Branch, Islamic Azad University, Zahedan, Iran
  11. 11. Department of Clinical Psychology, Zahedan University of Medical Sciences, Zahedan, Iran

Source: Acta Microbiologica et Immunologica Hungarica Published:2021


Abstract

Acinetobacter baumannii, as a nosocomial pathogen has become a worldwide concern in recent years. In the current study, the resistance to tetracyclines and colistin were assessed in the isolates from different provinces of Iran. During the timeline of this study, a number of 270 isolates of A. baumannii were collected from tracheal aspirates, wounds, urine and blood cultures. The minimum inhibitory concentration (MIC) for tetracycline, doxycycline, minocycline, tigecycline and colistin were evaluated. Tetracycline resistance genes were assessed by PCR. The mean expression level of adeB, adeJ and adeG were assessed using semi quantitative Real-Time PCR. The clonal relationship of the isolates was evaluated by the repetitive extragenic palindromic PCR (REP-PCR), International Clonal (IC) Lineage Multiplex PCR and multilocus sequence typing (MLST) (Pasteur scheme) methods. The MIC by microdilution method showed that 87.5, 51.4, 28, 0.74 and 0% of the isolates were resistant to tetracycline, doxycycline, minocycline, tigecycline and colistin respectively. The prevalence of tetracycline resistance genes was 99.2, 99.2, 98, 86.7, 10, 3.33, 0.37, 0% for adeB, adeJ, adeG, tetB, tetA(39), tetA, tetM and tetH in tetracycline-resistant isolates. Moreover, the expression level of adeB, adeJ, adeG genes in tigecycline-nonsusceptible A. baumannii (TNAB) strain was higher compared to the tigecycline-susceptible A. baumannii (TSAB). A broad genomic diversity was revealed, but ST2 was the most prevalent ST. Our results indicated that tetracycline resistance in Iran is mediated by resistance-nodulation-cell division (RND) and tetB efflux pumps. © 2021 Akademiai Kiado, Budapest
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