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The Effect of Fibronectin Coating on Protein Corona Structure and Cellular Uptake of Single-Walled Carbon Nanotubes Publisher



Mottaghitalab F1 ; Kiani M2 ; Farokhi M3 ; Dinarvand R1, 2 ; Ghodsollahi T4 ; Atyabi F1, 2
Authors
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Authors Affiliations
  1. 1. Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 14174, Iran
  3. 3. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
  4. 4. Nano Mabna Iranian Inc., P.O.Box 1676664116, Tehran, Iran

Source: Precision Nanomedicine Published:2020


Abstract

Protein coating, as an outstanding surface modification strategy, influence the organization of biomolecules in the interface of nanomaterials. In the present study, fibronectin (FN) was used to modify die surface chemistry of single-walled carbon nanotubes (SWNTs) and carboxylated SWNTs (CC>2-SWNTs) to analyze its effects on the protein corona composition and cellular uptake. At first, the successful coating of FN on the surface of both SWNTs was confirmed by transmission electron microscopy (TEM) and Raman spectroscopy. The results showed that the biomolecular organization of SWNTs and CCh-SWNTs coronas was changed after FN coating based on the evidence obtained from the surface plasmon intensity of the samples. Moreover, the MTT assay and confocal microscopy imaging revealed less cytotoxicity and cellular uptake of SWNTs coronas in comparison to bulk samples, respectively. It is suggested that protein coating of SWNTs can modify the corona pattern and consequently the biological behavior of carbon nanotubes. © 2020, Andover House, Inc.. All rights reserved.
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