Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Effects of Sub-Lethal Doses of Photo-Activated Disinfection Against Porphyromonas Gingivalis for Pharmaceutical Treatment of Periodontal-Endodontic Lesions Publisher Pubmed



Pourhajibagher M1, 2, 3 ; Chiniforush N2 ; Raoofian R4, 5 ; Ghorbanzadeh R6 ; Shahabi S7 ; Bahador A1, 2, 3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran
  5. 5. Innovative Research Center, Islamic Azad University, Mashhad Branch, Mashhad, Iran
  6. 6. Private Practice, Tehran, Iran
  7. 7. Dental Biomaterials Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran

Source: Photodiagnosis and Photodynamic Therapy Published:2016


Abstract

Background Microorganisms treated by photo-activated disinfection (PAD) in combined periodontal-endodontic (perio-endo) lesions would be exposed to sub-lethal doses of PAD (sPAD). This study evaluated the effect of sPAD using toluidine blue O (TBO) in combination with diode laser irradiation on the growth and biofilm-formation ability of Porphyromonas gingivalis as an endo-periodontal pathogen. Methods The antibacterial and antibiofilm potential of sPAD against P. gingivalis was analyzed at sub-lethal doses of TBO and irradiation time of diode laser on a colony-forming unit and crystal violet assays, respectively. Results TBO-mediated PAD, using 6.25–100 μg/mL at a fluency of 171.87 J/cm2 and 12.5–100 μg/mL at a fluency of 137.5 J/cm2, showed a significant dose-dependent reduction in P. gingivalis growth when compared to the control. TBO-mediated PAD showed a significantly inhibitory effect on biofilm formation in P. gingivalis than TBO-PAD at sub-lethal levels. Conclusion High doses of sPAD revealed antibacterial and antibiofilm potential activity, whereas lower doses of sPAD had conflicting results. Therefore, when PAD is prescribed in combined perio-endo lesions treatment, the dose of PAD used in vivo should be taken into account. © 2016 Elsevier B.V.
Related Docs
View other Related Docs
1. Evaluation of Photo-Activated Disinfection Effectiveness With Methylene Blue Against Porphyromonas Gingivalis Involved in Endodontic Infection: An in Vitro Study, Photodiagnosis and Photodynamic Therapy (2016)
2. Photo-Activated Disinfection Based on Indocyanine Green Against Cell Viability and Biofilm Formation of Porphyromonas Gingivalis, Photodiagnosis and Photodynamic Therapy (2017)
3. The Efficacy of Photodynamic and Photothermal Therapy on Biofilm Formation of Streptococcus Mutans: An in Vitro Study, Photodiagnosis and Photodynamic Therapy (2017)
4. The Effect of Antimicrobial Photodynamic Therapy on the Expression of Novel Methicillin Resistance Markers Determined Using Cdna-Aflp Approach in Staphylococcus Aureus, Photodiagnosis and Photodynamic Therapy (2017)
5. Real-Time Quantitative Reverse Transcription-Pcr Analysis of Expression Stability of Aggregatibacter Actinomycetemcomitans Fimbria-Associated Gene in Response to Photodynamic Therapy, Photodiagnosis and Photodynamic Therapy (2017)
6. An in Vivo Evaluation of Microbial Diversity Before and After the Photo-Activated Disinfection in Primary Endodontic Infections: Traditional Phenotypic and Molecular Approaches, Photodiagnosis and Photodynamic Therapy (2018)
7. The Evaluation of Cultivable Microbiota Profile in Patients With Secondary Endodontic Infection Before and After Photo-Activated Disinfection, Photodiagnosis and Photodynamic Therapy (2017)
8. Exploring Different Photosensitizers to Optimize Elimination of Planktonic and Biofilm Forms of Enterococcus Faecalis From Infected Root Canal During Antimicrobial Photodynamic Therapy, Photodiagnosis and Photodynamic Therapy (2018)
Experts (# of related papers)
View all Related Experts
Abbas Bahador (15)
Maryam Pourhajibagher (14)
Other Related Docs
9. The in Vitro Effect of Antimicrobial Photodynamic Therapy With Indocyanine Green on Enterococcus Faecalis: Influence of a Washing Vs Non-Washing Procedure, Photodiagnosis and Photodynamic Therapy (2016)
10. Outer Membrane Protein 100 of Aggregatibacter Actinomycetemcomitans Act As a Biopharmaceutical Target for Photodynamic Therapy: An in Silico Analysis, Photodiagnosis and Photodynamic Therapy (2016)
11. Comparison of Different Modes of Photo-Activated Disinfection Against Porphyromonas Gingivalis: An in Vitro Study, Photodiagnosis and Photodynamic Therapy (2020)
12. Antimicrobial Activity of Photodynamic Therapy in Combination With Colistin Against a Pan-Drug Resistant Acinetobacter Baumannii Isolated From Burn Patient, Photodiagnosis and Photodynamic Therapy (2017)
13. The Effect of Sublethal Photodynamic Therapy on the Expression of Enterococcal Surface Protein (Esp) Encoding Gene in Enterococcus Faecalis: Quantitative Real-Time Pcr Assessment, Photodiagnosis and Photodynamic Therapy (2018)
14. The Effect of Antimicrobial Photodynamic Therapy Using Chlorophyllin-Phycocyanin Mixture on Enterococcus Faecalis: The Influence of Different Light Sources, Applied Sciences (Switzerland) (2020)
15. The Effect of Indocyanine Green Loaded on a Novel Nano-Graphene Oxide for High Performance of Photodynamic Therapy Against Enterococcus Faecalis, Photodiagnosis and Photodynamic Therapy (2017)
16. Diagnostic Accuracy of Multiplex Real-Time Pcr Approaches Compared With Cultivation -Based Detection Methods: Monitoring the Endopathogenic Microbiota Pre and Post Photo-Activated Disinfection, Photodiagnosis and Photodynamic Therapy (2018)