Design and Synthesis of a Novel Nanocomposite Based on Magnetic Dopamine Nanoparticles for Purification of Α-Amylase From the Bovine Milk

Design and Synthesis of a Novel Nanocomposite Based on Magnetic Dopamine Nanoparticles for Purification of Α-Amylase From the Bovine Milk Publisher Pubmed

Eivazzadehkeihan R¹ ; Dogari H¹ ; Ahmadpour F¹ ; Aliabadi HAM^{2, 3} ; Radinekiyan F¹ ; Maleki A¹ ; Fard LS¹ ; Tahmasebi B⁴ ; Mojdehi MFP¹ ; Mahdavi M⁵

Show Affiliations

1. Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran, 16846-13114, Iran
2. Protein Chemistry Laboratory, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
3. Advanced Chemistry Studies Lab, Department of Chemistry, Toosi University of Technology, Tehran, K. N, Iran
4. School of Chemistry, College of Science, University of Tehran, Tehran, Iran
5. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Scientific Reports Published:2021

Abstract

In this paper, a novel nanocomposite based on magnetic nanoparticles decorated by dopamine were reported. Three modified magnetic nanocomposites by dopamine were offered with different type of linkers. The mentioned magnetic nanocomposites were applied to separate α-amylase protein from fresh bovine milk. All of the magnetic nanocomposites were characterized and investigated by using Fourier-transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, field-emission scanning microscope, X-ray diffraction pattern, and vibrating-sample magnetometer analyses. To investigate the purifying application, sodium dodecyl sulfate polyacrylamide gel electrophoresis, one-dimensional isoelectric focusing gel electrophoresis, and alpha-amylase activity assay were employed. With paying attention to factors such as yield of purification and concentration of separated protein by each of magnetic nanocomposite, it could be concluded that the length of linkers played an important role in α-amylase protein separation. According to the results, the best separation and purification of α-amylase protein with 49.83% recovery and 40.11-fold purification efficiency was related to longest length linker, 1,4-butanediol diglycidyl ether, because of considerable conjugation with nanocomposite. Also, docking calculation has shown that the binding energy is − 1.697 kcal/mol and ΔG = − 6.844 kcal/mol which result that the interaction process between dopamine and α-amylase protein is spontaneous. © 2021, The Author(s).

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Experts (# of related papers)

Mohammad Mahdavi (28)

Bagher Larijani (1)

Style	Citing Format
MLA	Eivazzadehkeihan R, et al.. "Design and Synthesis of a Novel Nanocomposite Based on Magnetic Dopamine Nanoparticles for Purification of Α-Amylase From the Bovine Milk." Scientific Reports, vol. 11, no. 1, 2021, pp. -.
APA	Eivazzadehkeihan R, Dogari H, Ahmadpour F, Aliabadi HAM, Radinekiyan F, Maleki A, Fard LS, Tahmasebi B, Mojdehi MFP, Mahdavi M (2021). Design and Synthesis of a Novel Nanocomposite Based on Magnetic Dopamine Nanoparticles for Purification of Α-Amylase From the Bovine Milk. Scientific Reports, 11(1), -.
Chicago	Eivazzadehkeihan R, Dogari H, Ahmadpour F, Aliabadi HAM, Radinekiyan F, Maleki A, Fard LS, Tahmasebi B, Mojdehi MFP, Mahdavi M. "Design and Synthesis of a Novel Nanocomposite Based on Magnetic Dopamine Nanoparticles for Purification of Α-Amylase From the Bovine Milk." Scientific Reports 11, no. 1 (2021): -.
Harvard	Eivazzadehkeihan R et al. (2021) 'Design and Synthesis of a Novel Nanocomposite Based on Magnetic Dopamine Nanoparticles for Purification of Α-Amylase From the Bovine Milk', Scientific Reports, 11(1), pp. -.
Vancouver	Eivazzadehkeihan R, Dogari H, Ahmadpour F, Aliabadi HAM, Radinekiyan F, Maleki A, et al.. Design and Synthesis of a Novel Nanocomposite Based on Magnetic Dopamine Nanoparticles for Purification of Α-Amylase From the Bovine Milk. Scientific Reports. 2021;11(1):-.
BibTex	@article{ author = {Eivazzadehkeihan R and Dogari H and Ahmadpour F and Aliabadi HAM and Radinekiyan F and Maleki A and Fard LS and Tahmasebi B and Mojdehi MFP and Mahdavi M}, title = {Design and Synthesis of a Novel Nanocomposite Based on Magnetic Dopamine Nanoparticles for Purification of Α-Amylase From the Bovine Milk}, journal = {Scientific Reports}, volume = {11}, number = {1}, pages = {-}, year = {2021} }
RIS	TY - JOUR AU - Eivazzadehkeihan R AU - Dogari H AU - Ahmadpour F AU - Aliabadi HAM AU - Radinekiyan F AU - Maleki A AU - Fard LS AU - Tahmasebi B AU - Mojdehi MFP AU - Mahdavi M TI - Design and Synthesis of a Novel Nanocomposite Based on Magnetic Dopamine Nanoparticles for Purification of Α-Amylase From the Bovine Milk JO - Scientific Reports VL - 11 IS - 1 SP - EP - PY - 2021 ER -

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