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Upregulated Long Non-Coding Rnas Tmpo-As1, Ddx11-As1, and Pole Gene Expression Predict Poor Prognosis in Head and Neck Squamous Cell Carcinoma (Hnscc) Publisher



Mokhtarinejad M1 ; Pirhoushiaran M1 ; Heidarzadehpilehrood R2 ; Hesami S3 ; Azmoudehardalan F4 ; Farahani AS3
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Authors Affiliations
  1. 1. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, 1417613151, Iran
  2. 2. Department of Obstetrics & Gynaecology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor, Serdang, 43400, Malaysia
  3. 3. Medical Genetic Ward, Imam Khomeini Hospital Complex, IKHC, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Pathology, Imam Khomeini Hospital Complex, IKHC, Tehran University of Medical Sciences, Tehran, Iran

Source: Gene Reports Published:2024


Abstract

Background: Head and neck squamous cell carcinoma (HNSCC) is prevalent and highly aggressive. HNSCC pathogenesis is characterized by the presence of long non-coding RNAs (lncRNAs) that lead to abnormal cellular functions such as proliferation, migration, and invasion. Methods: This study examined the expression levels of lncRNAs TMPO-AS1, DDX11-AS1, and the POLE gene in 100 HNSCC tumors and normal adjacent tumors (NATs). The analysis was performed using quantitative reverse transcription polymerase chain reaction (RT-qPCR) on fresh-frozen samples. Results: In HNSCC samples, tumor tissue exhibited markedly elevated levels of POLE expression compared to NATs (p < .001, 95 % CI: 2.410–3.170 vs. 0.9834–1.549). DDX11-AS1 expression was also significantly higher in HNSCC samples than in NATs (p < .001, 95 % CI: 1.879–3.250 vs. 1.029–1.731). Similarly, TMPO-AS1 levels were significantly elevated in HNSCC samples compared to NATs (p < .001, 95 % CI: 1.903–2.639 vs. 0.894–1.329). ROC curve diagnostic analyses revealed AUC values of 0.838 (95 % CI: 0.761–0.915) for POLE, 0.689 (95 % CI: 0.585–0.793) for DDX11-AS1, and 0.808 (95 % CI: 0.724–0.893) for TMPO-AS1. Notably, a substantial association was found between tumor grade and human papillomavirus levels with POLE and TMPO-AS1. Additionally, significant associations were observed between tumor stage and metastasis with DDX11-AS1 and TMPO-AS1 levels. Our findings further investigate a remarkable association between the combined effect of smoking and HPV positive infections and expression levels of POLE, DDX11-AS1, and TMPO-AS1. Our final results align with those from The Cancer Genome Atlas (TCGA), which included 520 HNSCC cases and 44 controls. Conclusions: Our data emphasize the potential significance of DDX11-AS1, TMPO-AS1, and POLE in the poor prognosis of HNSCC, indicating their contribution to tumor growth and progression. © 2024
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