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Dual Electrochemical Sensing of Spiked Virus and Sars-Cov-2 Using Natural Bed-Receptor (Mv-Gal1) Publisher Pubmed



Ghazizadeh E1, 2 ; Neshastehriz A2 ; Firoozabadi AD3 ; Yazdi MK4 ; Saievariranizad E5 ; Einali S2
Authors
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Authors Affiliations
  1. 1. Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  2. 2. Radiation Biology Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran
  3. 3. Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  4. 4. Department of Pediatric Hematologist and Oncologist, Bahrami Children Hospital, Tehran University of Medical Sciences, Tehran, 25529, Iran
  5. 5. Department of Physics, Faculty of Science, University of Tarbiat Modarres, Tehran, Iran

Source: Scientific Reports Published:2021


Abstract

It has been necessary to use methods that can detect the specificity of a virus during virus screening. In this study, we use a dual platform to identify any spiked virus and specific SARS-CoV-2 antigen, sequentially. We introduce a natural bed-receptor surface as Microparticle Vesicle-Galactins1 (MV-gal1) with the ability of glycan binding to screen every spiked virus. MV are the native vesicles which may have the gal-1 receptor. Gal-1 is the one of lectin receptor which can bind to glycan. After dropping the MV-gal1 on the SCPE/GNP, the sensor is turned on due to the increased electrochemical exchange with [Fe(CN)6]−3/−4 probe. Dropping the viral particles of SARS-CoV-2 cause to turn off the sensor with covering the sugar bond (early screening). Then, with the addition of Au/Antibody-SARS-CoV-2 on the MV-gal1@SARS-CoV-2 Antigen, the sensor is turned on again due to the electrochemical amplifier of AuNP (specific detection).For the first time, our sensor has the capacity of screening of any spike virus, and the specific detection of COVID-19 (LOD: 4.57 × 102 copies/mL) by using the natural bed-receptor and a specific antibody in the point of care test. © 2021, The Author(s).