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Improved Wound Healing of Diabetic Foot Ulcers Using Human Placenta-Derived Mesenchymal Stem Cells in Gelatin Electrospun Nanofibrous Scaffolds Plus a Platelet-Rich Plasma Gel: A Randomized Clinical Trial Publisher Pubmed



Meamar R1 ; Ghasemimobarakeh L2 ; Norouzi MR2 ; Siavash M3 ; Hamblin MR4 ; Fesharaki M1
Authors
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Authors Affiliations
  1. 1. Isfahan Clinical Toxicology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Textile Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran
  3. 3. Isfahan Endocrine and Metabolism Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Laser Research Centre, Faculty of Health Science, University of Johannesburg, Doornfontein, 2028, South Africa

Source: International Immunopharmacology Published:2021


Abstract

Aim: The effectiveness of nanofibers containing human placenta-derived mesenchymal stem cells (hPDMSCs) plus platelet-rich plasma (PRP) for healing of diabetic foot ulcers (DFUs) was investigated. Methods: hPDMSCs were isolated from human donor placentas, and cultured in electrospun gelatin nanofibrous scaffolds (GNS). Twenty-eight patients with DFUs were randomized into three groups in a 12-week trial: (A) Treated with hPDMSCs; (B) Treated with hPDMSCs after coating the ulcer with PRP gel; (C) Control group received standard wound care. Wound area and pain free walking distance were measured every 2 weeks. Results: Flow cytometry showed the expression of mesenchymal markers. SEM images and DAPI staining indicated significantly higher levels of hPDMSC proliferation on GNS after 3 and 7 days of culture. The MTS assay showed a significant increase in proliferation on GNS, compared to controls. Wound size reduction was 66% in group A, 71% in group B, and 36% in control group C. A significant difference in wound closure and pain-free walking distance was observed between groups A and B, compared to control group C (p < 0.05), but no difference between groups A and B. Biopsy of the implanted tissue showed the development of new capillary formation in groups A and B. Conclusion: Implantation of hPDMSCs in GNS accelerated wound healing and improved clinical parameters in DFU patients. © 2021
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