Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Effects of Crocin on Rotational Behavior, Lipid Peroxidation and Nitrite Levels in Rat’S Brain Striatum in an Experimental Model of Parkinson's Disease



Hosseini M1 ; Rajaei Z2 ; Alaei H3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Physiology, School of Medicine and Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Neurosciences Research Center and Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2015

Abstract

Background: Degeneration of the nigrostriatal dopaminergic system is the pathologic hallmark of Parkinson's disease (PD), which leads to movement disorders. Compelling evidence implicates that oxidative stress plays an important role in degeneration of dopaminergic neurons in the disease. The aim of this study was to investigate the neuroprotective effect of crocin, a potent antioxidant insaffron, in an experimental model of Parkinson's disease in rat. Methods: 32 adult male Wistar rats were randomly divided into 4 groups of 8 including sham, parkinsonian and parkinsonian treated with crocin at doses of 30 and 60 mg/kg. 6-hydroxydopamine (6-OHDA) (16 μg in 0.2% ascorbate-saline) was infused into the left medial forebrain bundle. Crocin was injected intraperitoneally from 3 days before the surgery until 6 weeks. Rats were tested for rotational behavior by injection of apomorphine hydrochloride (2 mg/kg, intraperitoneally) at the weeks2, 4 and 6. Malondialdehyde and nitrite levels were measured in the striatum at the end of the week 6. Findings: The apomorphine-induced contralateral body rotations were highly significant in parkinsonian group at the end of the weeks 2, 4 and 6 compared to the sham group (P < 0.001). Treatment of parkinsonian rats with crocin at the doses of 30 and 60 mg/kg did not change therotations compared to the parkinsonian group. Malondialdehyde and nitrite levels in the striatum were significantly increased in parkinsonian group compared to the sham group (P < 0.050). Treatment of parkinsonian rats with crocin at a dose of 60 mg/kg significantly decreased the nitrite levels in the striatum compared to the parkinsonian rats (P < 0.05). However, treatment with crocin had no effect on malondialdehyde levels in the striatum. Conclusion: According to the present study, it seems that crocin at a dose of 60 mg/kg is effective on preventing the nitrosative stress in an experimental model of Parkinson's disease. However, crocin has no effect on improvement of rotational behavior and oxidative damage. © 2015, Isfahan University of Medical Sciences(IUMS). All rights reserved.
Related Docs
View other Related Docs
1. The Effects of Crocin on 6-Ohda-Induced Oxidative/Nitrosative Damage and Motor Behaviour in Hemiparkinsonian Rats, Malaysian Journal of Medical Sciences (2016)
2. Pretreatment With Crocin Along With Treadmill Exercise Ameliorates Motor and Memory Deficits in Hemiparkinsonian Rats by Anti-Inflammatory and Antioxidant Mechanisms, Metabolic Brain Disease (2019)
3. Effects of Crocin on Brain Oxidative Damage and Aversive Memory in a 6-Ohda Model of Parkinson’S Disease; [Efeitos Da Crocina No Dano Oxidativo Cerebral E Na Memoria Aversiva Em Um Modelo 6-Ohda De Doenca De Parkinson], Arquivos de Neuro-Psiquiatria (2016)
4. Antihyperglycemic and Antioxidant Activity of Crocin in Streptozotocin-Induced Diabetic Rats, Journal of Medicinal Food (2013)
5. Crocin Improves Cognitive Impairment in Lps-Treated Rats Through Anti-Apoptotic, Anti-Inflammatory, and Antioxidant Activities, Molecular Neurobiology (2024)
6. Crocin Improves Spatial Learning and Memory Deficits in the Morris Water Maze Via Attenuating Cortical Oxidative Damage in Diabetic Rats, Neuroscience Letters (2017)
7. Effects of Carvacrol and Physical Exercise on Motor and Memory Impairments Associated With Parkinson’S Disease; [Efeitos Do Carvacrol E Do Exercicio Fisico Sobre O Comprometimento Motor E De Memoria Associados a Doenca De Parkinson], Arquivos de Neuro-Psiquiatria (2019)
8. Effects of Crocin on Spatial or Aversive Learning and Memory Impairments Induced by Lipopolysaccharide in Rats, Avicenna Journal of Phytomedicine (2021)
Experts (# of related papers)
View all Related Experts
Hojatallah Alaei (13)
Ziba Rajaei (11)
Other Related Docs
9. Effects of Crocin on Cognitive and Spatial Memories in Rats Under Chronic Isolation Stress, Physiology and Pharmacology (Iran) (2018)
10. Chronic Treatment With Carvacrol Improves Passive Avoidance Memory in a Rat Model of Parkinson’S Disease; [O Tratamento Com Carvacrol Melhora a Memoria De Esquiva Passiva Em Um Modelo Da Doenca De Parkinson Em Ratos], Arquivos de Neuro-Psiquiatria (2018)
11. The Impact of Sesamol and Exercise on Striatal Tnf-Α Level, Motor Behavior, Aversive Memory and Oxidative Stress Status in 6-Hydroxydopamine-Lesioned Rats, Physiology and Pharmacology (Iran) (2022)
12. Saffron and Sleep Quality: A Systematic Review of Randomized Controlled Trials, Nutrition and Metabolic Insights (2023)
13. Gallic Acid Ameliorates Lps-Induced Memory Decline by Modulating Nf-Κb, Tnf-Α, and Caspase 3 Gene Expression and Attenuating Oxidative Stress and Neuronal Loss in the Rat Hippocampus, Metabolic Brain Disease (2025)
14. The Effect of Preventive Exercise on the Neuroprotection in 6-Hydroxydopamine-Lesioned Rat Brain, Applied Physiology, Nutrition and Metabolism (2019)
15. Effects of Cinnamon Oil and Its Main Constituents, Cinnamic Acid and Cinnamaldehyde, on 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Induced Neurodegeneration in Pc-12 Cells; [Efectos Del Aceite De Canela Y Sus Principales Componentes, Acido Cinamico Y Cinamaldehido En La Neurodegeneracion Inducida Por 1-Metil-4-Fenil-1,2,3,6-Tetrahidropiridina En Celulas Pc 12], Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas (2023)
16. Exercise-Induced Neuroprotection in the 6-Hydroxydopamine Parkinson’S Disease Model, Neurotoxicity Research (2020)
17. The Effects of Red Grape Juice and Exercise on Parkinson’S Disease in Male Rats, Journal of Isfahan Medical School (2012)
18. The Effects of Synbiotic Supplementation on Oxidative Stress Markers, Mental Status, and Quality of Life in Patients With Parkinson's Disease: A Double-Blind, Placebo-Controlled, Randomized Controlled Trial, Journal of Functional Foods (2023)
19. Neuroprotective Effects of Endurance Training in 6-Hydroxydopamine Rat Model of Parkinson’S Disease, Koomesh (2020)
20. Therapeutic Effects of Saffron and Its Components on Neurodegenerative Diseases, Heliyon (2024)
21. Effects of Preventive Treadmill Exercise on the Recovery of Metabolic and Mitochondrial Factors in the 6-Hydroxydopamine Rat Model of Parkinson’S Disease, Neurotoxicity Research (2019)
22. Neuroprotective Actions of Norepinephrine in Neurological Diseases, Pflugers Archiv European Journal of Physiology (2024)
23. Comparative Study of the Protective Effects of Crocin and Exercise on Long-Term Potentiation of Ca1 in Rats Under Chronic Unpredictable Stress, Life Sciences (2020)
24. The Impact of Crocin and Chronic Isolation Stress on Passive Avoidance Memory and Brain Electrical Activity in Male Rats, Physiology and Pharmacology (Iran) (2022)
25. Role of Dietary Antioxidants and Redox Status in Parkinson's Disease, Diet and Nutrition in Neurological Disorders (2023)
26. Pre-Clinical Studies Identifying Molecular Pathways of Neuroinflammation in Parkinson's Disease: A Systematic Review, Frontiers in Aging Neuroscience (2022)
27. Comparing the Efficacy of Escitalopram With and Without Crocin in Restoring I/O Functions and Ltp Within the Hippocampal Ca1 Region of Stressed Rats, Advanced Biomedical Research (2024)
28. Silencing of Hsp70 Intensifies 6-Ohda-Induced Apoptosis and Hsp90 Upregulation in Pc12 Cells, Journal of Molecular Neuroscience (2015)