Evaluation of Interleukin 25 and Interleukin 25 Receptor Expression in Peripheral Blood Mononuclear Cells of Breast Cancer Patients and Normal Subjects

Evaluation of Interleukin 25 and Interleukin 25 Receptor Expression in Peripheral Blood Mononuclear Cells of Breast Cancer Patients and Normal Subjects Publisher Pubmed

Barati M^{1, 2} ; Sinaeian M³ ; Shokrollahi Barough M^{4, 5} ; Pak F⁶ ; Semnani V⁷ ; Kokhaei P^{6, 8, 9} ; Momtaziborojeni AA^{10, 11, 12}

Show Affiliations

1. Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2. Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran
3. Department of Biology Damghan Branch, Islamic Azad University, Damghan, Iran
4. Research Committee, Semnan University of Medical Sciences, Semnan, Iran
5. ATMP Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
6. Departments of Immunology, Semnan University of Medical Sciences, Semnan, 35147-99442, Iran
7. Departments of Pathology, Semnan University of Medical Sciences, Semnan, Iran
8. Department of Oncology-Pathology, Immune and Gene Therapy Lab, Cancer Center Karolinska (CCK), Karolinska University Hospital Solna and Karolinska Institute, Stockholm, Sweden
9. Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran
10. Halal Research Center of IRI, FDA, Tehran, Iran
11. Nanotechnology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
12. Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Source: Journal of Interferon and Cytokine Research Published:2020

Abstract

Color images are available online. Interleukin 25 (IL-25) is a ligand for IL-25 receptor (IL-25R) with apoptotic effect on breast cancer epithelial cells that are produced by peripheral blood mononuclear cells (PBMCs). In this study, we aimed to evaluate IL-25/IL-25R mRNA expression in PBMCs, and also investigate correlation of IL-25/IL-25R with tumor stages/grades in patients with breast cancer. PBMCs and serum were isolated from 30 patients with breast cancer and 18 normal subjects. ELISA test was conducted for IL-25 cytokine. Total RNA was isolated from 2 × 106 PBMCs and reverse transcribed to cDNA. Quantitative PCRs were performed for IL-25, IL-25R, and GAPDH genes. IL-25 mRNA expression in PBMCs of breast cancer patients (malignant and benign) was significantly lower than that in normal subjects, Also IL-25 expression in breast cancer patients with malignant tumor was significantly lower than that in nonmalignant patients. IL-25R expression in malignant patients was significantly higher than that of benign and normal subjects (P < 0.05). IL-25 in serum of normal subjects was higher than that of benign and malignant patients. There was a direct association between IL-25R expression and tumor grade/stage of cancer. In conclusion, IL-25 seems as a potential prognostic factor in the serum of breast cancer patients and reduction of IL-25 is associated with a higher grade/stage of cancer. © Mary Ann Liebert, Inc., publishers 2020.

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Style	Citing Format
MLA	Barati M, et al.. "Evaluation of Interleukin 25 and Interleukin 25 Receptor Expression in Peripheral Blood Mononuclear Cells of Breast Cancer Patients and Normal Subjects." Journal of Interferon and Cytokine Research, vol. 40, no. 3, 2020, pp. 139-144.
APA	Barati M, Sinaeian M, Shokrollahi Barough M, Pak F, Semnani V, Kokhaei P, Momtaziborojeni AA (2020). Evaluation of Interleukin 25 and Interleukin 25 Receptor Expression in Peripheral Blood Mononuclear Cells of Breast Cancer Patients and Normal Subjects. Journal of Interferon and Cytokine Research, 40(3), 139-144.
Chicago	Barati M, Sinaeian M, Shokrollahi Barough M, Pak F, Semnani V, Kokhaei P, Momtaziborojeni AA. "Evaluation of Interleukin 25 and Interleukin 25 Receptor Expression in Peripheral Blood Mononuclear Cells of Breast Cancer Patients and Normal Subjects." Journal of Interferon and Cytokine Research 40, no. 3 (2020): 139-144.
Harvard	Barati M et al. (2020) 'Evaluation of Interleukin 25 and Interleukin 25 Receptor Expression in Peripheral Blood Mononuclear Cells of Breast Cancer Patients and Normal Subjects', Journal of Interferon and Cytokine Research, 40(3), pp. 139-144.
Vancouver	Barati M, Sinaeian M, Shokrollahi Barough M, Pak F, Semnani V, Kokhaei P, et al.. Evaluation of Interleukin 25 and Interleukin 25 Receptor Expression in Peripheral Blood Mononuclear Cells of Breast Cancer Patients and Normal Subjects. Journal of Interferon and Cytokine Research. 2020;40(3):139-144.
BibTex	@article{ author = {Barati M and Sinaeian M and Shokrollahi Barough M and Pak F and Semnani V and Kokhaei P and Momtaziborojeni AA}, title = {Evaluation of Interleukin 25 and Interleukin 25 Receptor Expression in Peripheral Blood Mononuclear Cells of Breast Cancer Patients and Normal Subjects}, journal = {Journal of Interferon and Cytokine Research}, volume = {40}, number = {3}, pages = {139-144}, year = {2020} }
RIS	TY - JOUR AU - Barati M AU - Sinaeian M AU - Shokrollahi Barough M AU - Pak F AU - Semnani V AU - Kokhaei P AU - Momtaziborojeni AA TI - Evaluation of Interleukin 25 and Interleukin 25 Receptor Expression in Peripheral Blood Mononuclear Cells of Breast Cancer Patients and Normal Subjects JO - Journal of Interferon and Cytokine Research VL - 40 IS - 3 SP - 139 EP - 144 PY - 2020 ER -

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