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Nano Conjugated Plga-Chlorambucil: Synthesis in Vitro Anti Non-Hodgkin's Lymphoma Cellular Assay Publisher



Akbarian S2 ; Sojoodi J2 ; Monnavari F1 ; Heidari H3 ; Khosravian P3 ; Javar HA3 ; Assadi A2 ; Rasouli R4 ; Saffari M5 ; Shandiz SA6 ; Hejazinia H2 ; Omoomi FD2 ; Saffari M5 ; Ardestani MS2, 8
Authors
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Authors Affiliations
  1. 1. Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
  2. 2. Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Medical Nanotechnologies in Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Herbal Medicine Research Center (HMRC), Department of Pharmaceutics, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
  6. 6. Young Researchers and Elite Club, East Tehran Branch, Islamic Azad University, Tehran, Iran
  7. 7. School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  8. 8. Departments of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: Letters in Drug Design and Discovery Published:2017


Abstract

Background: In spite of increasing number of chemotherapeutic drugs, achieving chemotherapy drug with minimal side effects in cancer treatment is still a major challenge. Chemotherapy has an important role in the treatment of non- Hodgkin's lymphoma cancer. Chlorambucil (CBL) is a lipophilic DNA alkylating drug having been administrated in many cancers like leukemia but its use has been limited because of chemical instability, low permeability of the cells and high toxicity. Objective: The main aim of this study is improving in vitro anticancer activity of CBL through conjugating CBL with Poly (DL-lactide-co-glycolide) (PLGA). The characterization of physiochemical structure of PLGA-CBL conjugation was determined by different techniques such as dynamic light scattering (DLS), Scanning electron microscopy (SEM), Atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR), and HNMR Spectrometry. Moreover, therapeutic effect of new PLGA-CBL conjugated was evaluated, after which, the cell viability was determined by MTT assay and the numbers of apoptotic/necrotic cells were calculated by flowcytometry using Annexin V/PIkit on a non- Hodgkin's lymphoma cell line. Results and Conclusion: The results of in vitro cytotoxicity showed significantly greater conjugated PLGA-CBL on the non-Hodgkin's lymphoma compared to CBL alone ©2017 Bentham Science Publishers
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