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Preparation and in Vitro Evaluation of Novel Cross-Linked Chondroitin Sulphate Nanoparticles by Aluminium Ions for Encapsulation of Green Tea Flavonoids Publisher Pubmed



Varshosaz J1 ; Asefi H2 ; Hashemibeni B3 ; Ghaffari S2 ; Davoudi A4
Authors
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Authors Affiliations
  1. 1. Department of Pharmaceutics, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Medical Nanotechnology, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran
  3. 3. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Medical Nanotechnology, Shiraz University of Medical Sciences, Shiraz, Iran

Source: IET Nanobiotechnology Published:2018


Abstract

Chondroitin sulphate is a sulphated glycosaminoglycan biopolymer composed over 100 individual sugars. Chondroitin sulphate nanoparticles (NPs) loaded with catechin were prepared by an ionic gelation method using AlCl3 and optimised for polymer and cross-linking agent concentration, curing time and stirring speed. Zeta potential, particle size, loading efficiency, and release efficiency over 24 h (RE24%) were evaluated. The surface morphology of NPs was investigated by scanning electron microscopy and their thermal behaviour by differential scanning calorimetric. Antioxidant effect of NPs was determined by chelating activity of iron ions. The cell viability of mesenchymal stem cells was determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide assay and the calcification of osteoblasts was studied by Alizarin red staining. The optimised NPs showed particle size of 176 nm, zeta potential of -20.8 mV, loading efficiency of 93.3% and RE24% of 80.6%. The chatechin loaded chondroitin sulphate NPs showed 70-fold more antioxidant activity, 3-fold proliferation effect and higher calcium precipitation in osteoblasts than free catechin. © The Institution of Engineering and Technology 2018.
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