Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Injectable Gellan Gum/Lignocellulose Nanofibrils Hydrogels Enriched With Melatonin Loaded Forsterite Nanoparticles for Cartilage Tissue Engineering: Fabrication, Characterization and Cell Culture Studies Publisher Pubmed



Kouhi M1 ; Varshosaz J1, 2 ; Hashemibeni B3 ; Sarmadi A4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Pharmaceutics, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Materials Science and Engineering C Published:2020


Abstract

Injectable hydrogels based on natural polysaccharides have attracted considerable attention in cartilage tissue engineering, especially those reinforced with mineral nanofilers carrying drug molecules. Here, a novel injectable hydrogel based on gellan gum (GG)/lignocellulose nanofibrils (LGNF) composite enriched with melatonin (MEL) loaded forsterite (FS) nanoparticles (FS-MEL) was developed to yield enhanced mechanical and biological properties of the hydrogels. Gelation time and temperature were determined for different hydrogel formulation containing 1–5 w/v% LGNF and 0.1–0.3 w/v% FS-MEL. The injectability test proved the ease of injection of the developed hydrogels. Degradation rate and swelling degree of developed hydrogel were evaluated to determine the effect of LGNF and FS on hydrogel behaviour. Results of mechanical characterization showed that the compressive modulus and strength of GG hydrogels were improved by incorporation of LGNF and FS. The results of MEL release study in PBS revealed that MEL showed more sustained release from the hydrogel compared to FS nanoparticles. Cell-hydrogels interaction was evaluated by culturing chondrocyte cells. Results exhibited higher cell adhesion, proliferation and gene expression on GG/LGNF/FS-MEL hydrogel compared to GG/LGNF and GG/LGNF/FS, which can be attributed to the synergic effect of FS and MEL. Overall results demonstrated that the developed GG/LGNF/FS-MEL hydrogels can be offered as promising materials for cartilage regeneration applications. © 2020
Other Related Docs
22. The Beneficial Effect of Encapsulated Human Adipose-Derived Stem Cells in Alginate Hydrogel on Neural Differentiation, Journal of Biomedical Materials Research - Part B Applied Biomaterials (2014)
25. Nanobiomaterials in Periodontal Tissue Engineering, Nanobiomaterials in Hard Tissue Engineering: Applications of Nanobiomaterials (2016)
50. Effects of Nanozeolite/Starch Thermoplastic Hydrogels on Wound Healing, Journal of Research in Medical Sciences (2017)