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Chromosome-Scale Echinococcus Granulosus (Genotype G1) Genome Reveals the Eg95 Gene Family and Conservation of the Eg95-Vaccine Molecule Publisher Pubmed



Korhonen PK1 ; Kinkar L1 ; Young ND1 ; Cai H3 ; Lightowlers MW1 ; Gauci C1 ; Jabbar A1 ; Chang BCH1 ; Wang T1 ; Hofmann A1 ; Koehler AV1 ; Li J3 ; Li J3 ; Wang D3 Show All Authors
Authors
  1. Korhonen PK1
  2. Kinkar L1
  3. Young ND1
  4. Cai H3
  5. Lightowlers MW1
  6. Gauci C1
  7. Jabbar A1
  8. Chang BCH1
  9. Wang T1
  10. Hofmann A1
  11. Koehler AV1
  12. Li J3
  13. Li J3
  14. Wang D3
  15. Yin J3
  16. Yang H3
  17. Jenkins DJ4
  18. Saarma U5
  19. Laurimae T5
  20. Rostaminejad M6
  21. Irshadullah M7
  22. Mirhendi H8
  23. Sharbatkhori M9
  24. Poncegordo F10
  25. Simsek S11
  26. Casulli A12
  27. Zait H13
  28. Atoyan H14
  29. De La Rue ML15
  30. Romig T16
  31. Wassermann M16
  32. Aghayan SA17
  33. Gevorgyan H18
  34. Yang B19
  35. Gasser RB1
Show Affiliations
Authors Affiliations
  1. 1. Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, 3010, VIC, Australia
  2. 2. BGI-Shenzhen, Shenzhen, 518083, China
  3. 3. Shenzhen Key Laboratory of Unknown Pathogen Identification, BGI-Shenzhen, Shenzhen, 518083, China
  4. 4. School of Animal and Veterinary Sciences, Charles Sturt University, Locked Bag 588, Wagga Wagga, 2678, NSW, Australia
  5. 5. Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, J. Liivi 2, Tartu, 50409, Estonia
  6. 6. Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  7. 7. Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh, 202002, India
  8. 8. Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  9. 9. Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran
  10. 10. Department of Parasitology, Faculty of Pharmacy, Complutense University, Plaza Ramon y Cajal s/n, Madrid, 28040, Spain
  11. 11. Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, Elazig, 23119, Turkey
  12. 12. World Health Organization Collaborating Centre for the Epidemiology, Detection and Control of Cystic and Alveolar Echinococcosis, European Union Reference Laboratory for Parasites (EURLP), Istituto Superiore di Sanita, Viale Regina Elena 299, Rome, 00161, Italy
  13. 13. Parasitology and Mycology Department, Mustapha University Hospital, Algiers, 16000, Algeria
  14. 14. Yerevan State University, Department of Zoology, Alex Manoogian, Yerevan, 0025, Armenia
  15. 15. Universidade Federal de Santa Maria, Departamento de Microbiologia e Parasitologia, RS, Santa Maria, Brazil
  16. 16. Institute of Zoology, Parasitology Unit, University of Hohenheim, Stuttgart, 70599, Germany
  17. 17. Chair of Zoology, Yerevan State University, 1 Alex Manoogian, Yerevan, 0025, Armenia
  18. 18. Molecular Parasitology Research Group, Scientific Center – Zoology and Hydroecology, 7P. Sevak str, Yerevan, 0014, Armenia
  19. 19. BGI Australia, Oceania, BGI Group, CBCRB Building, Herston Road, Herston, 4006, QLD, Australia

Source: Communications Biology Published:2022


Abstract

Cystic echinococcosis is a socioeconomically important parasitic disease caused by the larval stage of the canid tapeworm Echinococcus granulosus, afflicting millions of humans and animals worldwide. The development of a vaccine (called EG95) has been the most notable translational advance in the fight against this disease in animals. However, almost nothing is known about the genomic organisation/location of the family of genes encoding EG95 and related molecules, the extent of their conservation or their functions. The lack of a complete reference genome for E. granulosus genotype G1 has been a major obstacle to addressing these areas. Here, we assembled a chromosomal-scale genome for this genotype by scaffolding to a high quality genome for the congener E. multilocularis, localised Eg95 gene family members in this genome, and evaluated the conservation of the EG95 vaccine molecule. These results have marked implications for future explorations of aspects such as developmentally-regulated gene transcription/expression (using replicate samples) for all E. granulosus stages; structural and functional roles of non-coding genome regions; molecular ‘cross-talk’ between oncosphere and the immune system; and defining the precise function(s) of EG95. Applied aspects should include developing improved tools for the diagnosis and chemotherapy of cystic echinococcosis of humans. © 2022, The Author(s).
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