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Therapeutic Potentials of the Caffeine in Polycystic Ovary Syndrome in a Rat Model: Via Modulation of Proinflammatory Cytokines and Antioxidant Activity Publisher Pubmed



Raoofi A1, 2 ; Rezaie MJ3 ; Delbari A1, 2 ; Ghoreishi SAH4 ; Sichani PH5 ; Maleki S6 ; Nasiry D7 ; Akhlaghi M4 ; Ebrahimi V8 ; Khaneghah AM9
Authors
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Authors Affiliations
  1. 1. Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
  2. 2. Department of Anatomy, Sabzevar University of Medical Sciences, Sabzevar, Iran
  3. 3. Department of Anatomical Sciences, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
  4. 4. Student Research Committee, Sabzevar University of Medical Sciences, Sabzevar, Iran
  5. 5. Department of Nutrition Science, Isfahan Medical University, Isfahan, Iran
  6. 6. Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
  7. 7. Department of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  8. 8. Department of Anatomy, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  9. 9. Department of Fruit and Vegetable Product Technology, Prof. Waclaw Dabrowski Institute of Agricultural and Food Biotechnology— State Research Institute, Warsaw, Poland

Source: Allergologia et Immunopathologia Published:2022


Abstract

Recent studies have shown that polycystic ovary syndrome (PCOS) affects about 6% of women worldwide. It is associated with reproductive and metabolic dysfunction. Caffeine is naturally found in tea, cocoa, and coffee. It has been shown that caffeine can change hormonal profiles, stimulate ovulation, and enhance fertility. Therefore, in this study, the effects of caffeine on rats with PCOS were investigated. For this purpose, 40 female rats were divided into five groups: (1) control group (without any intervention), (2) sham group (administration of olive oil as a caffeine solvent), (3) PCOS group (injection of 2 mg of estradiol valerate for each rat), (4) caffeine group (administration of 37.5 mg/kg caffeine for each rat), and (5) PCOS + caffeine group. After 21 days of treatment, the ovaries of rats were removed and prepared for further evaluations, including hematoxylin and eosin staining, TUNEL assay, real-time PCR, and biochemical analysis. Administration of caffeine in PCOS mice considerably reduced both the volume of the ovary (P < 0.05) and follicular clusters (P < 0.01). However, superoxide dismutase and glutathione peroxidase were dramatically active in the PCOS + caffeine group compared to others (P < 0.05). Besides, caffeine treatment in PCOS mice led to Bax reduction and increased Bcl-2 expression. On the other hand, the expression of IL-6 and TNF-α in PCOS + caffeine group was high compared to other groups. We found that caffeine can reduce apoptosis and inflammation in PCOS ovaries and enhance the unpleasant symptoms of PCOS. © 2022 Codon Publications. Published by Codon Publications.
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