Analysis of Nad2 and Nad5 Enables Reliable Identification of Genotypes G6 and G7 Within the Species Complex Echinococcus Granulosus Sensu Lato

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Analysis of Nad2 and Nad5 Enables Reliable Identification of Genotypes G6 and G7 Within the Species Complex Echinococcus Granulosus Sensu Lato Publisher Pubmed

Laurimae T¹ ; Kinkar L¹ ; Romig T² ; Umhang G³ ; Casulli A^{4, 5} ; Omer RA⁶ ; Sharbatkhori M⁷ ; Mirhendi H⁸ ; Poncegordo F⁹ ; Lazzarini LE¹⁰ ; Soriano SV¹⁰ ; Varcasia A¹¹ ; Rostaminejad M¹² ; Andresiuk V¹³ Show All Authors

Laurimae T¹
Kinkar L¹
Romig T²
Umhang G³
Casulli A^{4, 5}
Omer RA⁶
Sharbatkhori M⁷
Mirhendi H⁸
Poncegordo F⁹
Lazzarini LE¹⁰
Soriano SV¹⁰
Varcasia A¹¹
Rostaminejad M¹²
Andresiuk V¹³
Maravilla P¹⁴
Gonzalez LM¹⁵
Dybicz M¹⁶
Gawor J¹⁷
Sarkunas M¹⁸
Snabel V¹⁹
Kuzmina T²⁰
Kia EB²¹
Saarma U¹

Source: Infection, Genetics and Evolution Published:2019

Abstract

The larval stages of tapeworms in the species complex Echinococcus granulosus sensu lato cause a zoonotic disease known as cystic echinococcosis (CE). Within this species complex, genotypes G6 and G7 are among the most common genotypes associated with human CE cases worldwide. However, our understanding of ecology, biology and epidemiology of G6 and G7 is still limited. An essential first step towards this goal is correct genotype identification, but distinguishing genotypes G6 and G7 has been challenging. A recent analysis based on complete mitogenome data revealed that the conventional sequencing of the cox1 (366 bp) gene fragment mistakenly classified a subset of G7 samples as G6. On the other hand, sequencing complete mitogenomes is not practical if only genotype or haplogroup identification is needed. Therefore, a simpler and less costly method is required to distinguish genotypes G6 and G7. We compared 93 complete mitogenomes of G6 and G7 from a wide geographical range and demonstrate that a combination of nad2 (714 bp) and nad5 (680 bp) gene fragments would be the best option to distinguish G6 and G7. Moreover, this method allows assignment of G7 samples into haplogroups G7a and G7b. However, due to very high genetic variability of G6 and G7, we suggest to construct a phylogenetic network based on the nad2 and nad5 sequences in order to be absolutely sure in genotype assignment. For this we provide a reference dataset of 93 concatenated nad2 and nad5 sequences (1394 bp in total) containing representatives of G6 and G7 (and haplogroups G7a and G7b), which can be used for the reconstruction of phylogenetic networks. © 2019 Elsevier B.V.

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Style	Citing Format
MLA	Laurimae T, et al.. "Analysis of Nad2 and Nad5 Enables Reliable Identification of Genotypes G6 and G7 Within the Species Complex Echinococcus Granulosus Sensu Lato." Infection, Genetics and Evolution, vol. 74, no. , 2019, pp. -.
APA	Laurimae T, Kinkar L, Romig T, Umhang G, Casulli A, Omer RA, Sharbatkhori M, Mirhendi H, Poncegordo F, Lazzarini LE, Soriano SV, Varcasia A, Rostaminejad M, Andresiuk V, Maravilla P, Gonzalez LM, Dybicz M, Gawor J, Sarkunas M, ... Saarma U (2019). Analysis of Nad2 and Nad5 Enables Reliable Identification of Genotypes G6 and G7 Within the Species Complex Echinococcus Granulosus Sensu Lato. Infection, Genetics and Evolution, 74(), -.
Chicago	Laurimae T, Kinkar L, Romig T, Umhang G, Casulli A, Omer RA, Sharbatkhori M, et al.. "Analysis of Nad2 and Nad5 Enables Reliable Identification of Genotypes G6 and G7 Within the Species Complex Echinococcus Granulosus Sensu Lato." Infection, Genetics and Evolution 74, no. (2019): -.
Harvard	Laurimae T et al. (2019) 'Analysis of Nad2 and Nad5 Enables Reliable Identification of Genotypes G6 and G7 Within the Species Complex Echinococcus Granulosus Sensu Lato', Infection, Genetics and Evolution, 74(), pp. -.
Vancouver	Laurimae T, Kinkar L, Romig T, Umhang G, Casulli A, Omer RA, et al.. Analysis of Nad2 and Nad5 Enables Reliable Identification of Genotypes G6 and G7 Within the Species Complex Echinococcus Granulosus Sensu Lato. Infection, Genetics and Evolution. 2019;74():-.
BibTex	@article{ author = {Laurimae T and Kinkar L and Romig T and Umhang G and Casulli A and Omer RA and Sharbatkhori M and Mirhendi H and Poncegordo F and Lazzarini LE and Soriano SV and Varcasia A and Rostaminejad M and Andresiuk V and Maravilla P and Gonzalez LM and Dybicz M and Gawor J and Sarkunas M and Snabel V and Kuzmina T and Kia EB and Saarma U}, title = {Analysis of Nad2 and Nad5 Enables Reliable Identification of Genotypes G6 and G7 Within the Species Complex Echinococcus Granulosus Sensu Lato}, journal = {Infection, Genetics and Evolution}, volume = {74}, number = {}, pages = {-}, year = {2019} }
RIS	TY - JOUR AU - Laurimae T AU - Kinkar L AU - Romig T AU - Umhang G AU - Casulli A AU - Omer RA AU - Sharbatkhori M AU - Mirhendi H AU - Poncegordo F AU - Lazzarini LE AU - Soriano SV AU - Varcasia A AU - Rostaminejad M AU - Andresiuk V AU - Maravilla P AU - Gonzalez LM AU - Dybicz M AU - Gawor J AU - Sarkunas M AU - Snabel V AU - Kuzmina T AU - Kia EB AU - Saarma U TI - Analysis of Nad2 and Nad5 Enables Reliable Identification of Genotypes G6 and G7 Within the Species Complex Echinococcus Granulosus Sensu Lato JO - Infection, Genetics and Evolution VL - 74 IS - SP - EP - PY - 2019 ER -