Effects of Hydroalcoholic Extract of Juglans Regia Leaves on Activity of Ast and Alt Enzymes in Alloxan- Induced Diabetic Rats



Madani H¹ ; Rahimi P¹ ; Mahzoni P² Show Affiliations
Source: Pharmaceutical Sciences Published:2009

Abstract

Objectives: Diabetes is a metabolic disorder characterised by hyperglycemia resulting from perturbation in insulin secretion, insulin action or both. Juglans regia (Juglandaceae) has been used in Iranian traditional medicine in the treatment of diabetes. In this experience the preventive effect of hydroalcoholic extract oiJuglans regia leaves on activity of AST and ALT in alloxan - induced diabetic rats was investigated. Methods: In this research 18 male white Wistar rats, with body weights of 180-220 g were randomly allocated into three groups with six rats per group:first group(nondiabetic control); second group(diabetic control) and third group(diabetic rats treated with hydroalcoholic extract of Juglans regia leaves). In order to induce diabetes, alloxan was administered as a single dose(120 mgkg- 1BW), intraperitoneally. At the initial and the end of 2 and 6 weeks of experimental period, rats were fasted for 8h, and then fasting blood samples were collected in heparinated tubes. Sampling was performed from the orbital sinus. Results: The results indicate a significant difference in AST and ALT level in the diabetic group compared with the other groups (P<0.05). Histological studies of the liver of these animals, demonstrated the same results. Conclusion: These results show that the hydroalcoholic extract of Juglans regia leaves could affects on preventive of diabetes.