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Breast Cancer Cell Targeted Mr Molecular Imaging Probe: Anti-Muc1 Antibody-Based Magnetic Nanoparticles Publisher



Khaniabadi PM1 ; Majid AMSA2 ; Asif M2 ; Khaniabadi BM3 ; Shahbazigahrouei D4 ; Jaafar MS1
Authors
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Authors Affiliations
  1. 1. School of Physics, University Science Malaysia, Pulau Penang, 11800, Malaysia
  2. 2. EMAN Research and Testing Laboratory, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, 11800, Malaysia
  3. 3. Child Growth and Development Research Centre, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Medical Physics, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran

Source: Journal of Physics: Conference Series Published:2017


Abstract

Effective and specific diagnostic imaging techniques are important in early-stage breast cancer treatment. The objective of this study was to develop a specific breast cancer contrast agent for magnetic resonance imaging (MRI). In so doing, superparamagnetic iron oxide nanoparticles (SPIONs) were conjugated to C595 monoclonal antibody using EDC chemistry to produce nanoprobe with high relaxivity and narrow size (87.4±0.7 nm). To test the developed nanoprobe in vitro, assessments including Cell toxicity, targeting efficacy, cellular binding, and MR imaging were carried out. The results indicated that after 6 hrs incubation with MCF-7 cells at 200 to 25 μg Fe/ml doses, 76% to 16% T2 reduction was obtained. The presence of iron localised in MCF-7 cells measured by atomic absorption spectroscopy (AAS) was about 9.95±0.09 ppm iron/cell at higher doses of nanoprobe. Moreover, a linear relationship between iron concentration of nontoxic SPION-C595 and T2 relaxation times was observed. This study also revealed that developed nanoprobe might be used as a specific negative contrast agent for detecting breast cancer. © Published under licence by IOP Publishing Ltd.
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