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A Natural Nanocomposite Based on Gelatin-Collagen Hydrogel and Silk Fibroin Embedded With Znfe Ldh for Biological Applications Publisher



Sehat S1 ; Aliabadi HAM2 ; Mahdian S1 ; Kashtiaray A1 ; Sadat Z1 ; Mahdavi M3 ; Eivazzadehkeihan R1 ; Maleki A1 ; Madanchi H4, 5
Authors
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Authors Affiliations
  1. 1. Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran, 16846-13114, Iran
  2. 2. Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
  3. 3. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Biotechnology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran
  5. 5. Drug Design and Bioinformatics Unit, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran

Source: Process Biochemistry Published:2024


Abstract

In this research, a novel hydrogel nanobiocomposite was developed by combining natural Collagen (Col) and Gelatin (Ge) biopolymers with Glutaraldehyde (GA), forming a cross-linked Col-Ge hydrogel. This hydrogel was further enhanced by adding Silk fibroin (SF) protein and ZnFe layered double hydroxide (LDH) for improved mechanical properties and antibacterial functionality. Various analytical techniques were employed to comprehensively characterize the structural features of the designed Col-Ge hydrogel/SF/ZnFe LDH nanocomposite. Biological tests, including MTT and hemolytic assays, demonstrated 95.25 % and 96.51 % cell viability for human skin fibroblast cells (Hu02) after 2 and 3 days. Also, in addition to non-toxicity, the fibroblast shape of Hu02 cells was entirely maintained. The nanocomposite exhibited only 4.83 %hemolytic activity on human RBCs, indicating excellent hemocompatibility. In inhibiting bacterial growth, it showed 27.51 % inhibition for E. coli and 16.8 % for S. aureus. These findings suggest that the Col-Ge hydrogel/SF/ZnFe LDH nanobiocomposite holds promise for biomedical applications. © 2024 Elsevier Ltd
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