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Platelet Rich Fibrin and Simvastatin-Loaded Pectin-Based 3D Printed-Electrospun Bilayer Scaffold for Skin Tissue Regeneration Publisher Pubmed



Tavakoli M1 ; Almusawi MH2 ; Kalali A3 ; Shekarchizadeh A4 ; Kaviani Y5 ; Mansouri A6 ; Nasiriharchegani S7 ; Kharazi AZ8 ; Sharifianjazi F9 ; Sattar M10 ; Varshosaz J11 ; Mehrjoo M12 ; Najafinezhad A7 ; Mirhaj M1
Authors
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Authors Affiliations
  1. 1. Department of Materials Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran
  2. 2. Department of Clinical Laboratory Science, College of Pharmacy, Mustansiriyah University, Baghdad, Iraq
  3. 3. School of Metallurgy and Materials Engineering, Iran University of Science & Technology, Tehran, Iran
  4. 4. Department of Genetics, Faculty of Science, Shahrekord University, Shahrekord, Iran
  5. 5. Department of Biomedical Engineering, University of Meybod, Yazd, Iran
  6. 6. Department of Biology, Isfahan University, Isfahan, Iran
  7. 7. Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran
  8. 8. Department of Biomaterials, Nanotechnology and Tissue Engineering, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Iran
  9. 9. Department of Natural Sciences, School of Science and Technology, University of Georgia, Tbilisi, 0171, Georgia
  10. 10. Research group of Microbiological Engineering and Medical Materials, College of Biological Science and Medical Engineering, Donghua University, No. 2999 North Renmin Road, Shanghai, 201620, China
  11. 11. Novel Drug Delivery Systems Research Centre, Department of Pharmaceutics, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
  12. 12. Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran

Source: International Journal of Biological Macromolecules Published:2024


Abstract

Designing multifunctional wound dressings is a prerequisite to prevent infection and stimulate healing. In this study, a bilayer scaffold (BS) with a top layer (TL) comprising 3D printed pectin/polyacrylic acid/platelet rich fibrin hydrogel (Pec/PAA/PRF) and a bottom nanofibrous layer (NL) containing Pec/PAA/simvastatin (SIM) was produced. The biodegradable and biocompatible polymers Pec and PAA were cross-linked to form hydrogels via Ca2+ activation through galacturonate linkage and chelation, respectively. PRF as an autologous growth factor (GF) source and SIM together augmented angiogenesis and neovascularization. Because of 3D printing, the BS possessed a uniform distribution of PRF in TL and an average fiber diameter of 96.71 ± 18.14 nm was obtained in NL. The Young's modulus of BS was recorded as 6.02 ± 0.31 MPa and its elongation at break was measured as 30.16 ± 2.70 %. The wound dressing gradually released growth factors over 7 days of investigation. Furthermore, the BS significantly outperformed other groups in increasing cell viability and in vivo wound closure rate (95.80 ± 3.47 % after 14 days). Wounds covered with BS healed faster with more collagen deposition and re-epithelialization. The results demonstrate that the BS can be a potential remedy for skin tissue regeneration. © 2024 Elsevier B.V.
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