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Crispr/Cas9-Engineered Mesenchymal Stromal/Stem Cells and Their Extracellular Vesicles: A New Approach to Overcoming Cell Therapy Limitations Publisher Pubmed



Hazrati A1 ; Malekpour K2 ; Soudi S3 ; Hashemi SM4, 5
Authors
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Authors Affiliations
  1. 1. Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  4. 4. Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Biomedicine and Pharmacotherapy Published:2022


Abstract

Cell therapy is one of the newest therapeutic approaches for treating tissue destruction diseases and replacing damaged parts in defective tissues. Among different cells, mesenchymal stem cells (MSCs) have received a lot of attention due to their advantages and desirable properties. Also, MSCs-derived secretome, which includes various growth factors, cytokines, and extracellular vesicles (EVs), is used in the treatment of different types of diseases. However, the application of MSCs in an intact form brings their functionality with limitations. For this reason, different methods are recommended to increase their efficiency and the extracellular vesicles derived from them. One of these methods is gene editing of these cells. Among the different techniques for MSCs gene editing, CRISPR/Cas9 can increase the therapeutic potential of MSCs in a targeted manner due to its advantages. In order to achieve the desired result, various genes have been manipulated in MSCs, including genes involved in stemness, aging, migration, proliferation, survival, and inflammatory responses. Engineering MSCs with this method affects the cells' characteristics, changes their cytokine and different growth factors secretions, and increases their therapeutic efficiency. © 2022 The Authors
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