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Sars-Cov-2 Mrna-Vaccine Candidate; Corenapcin®, Induces Robust Humoral and Cellular Immunity in Mice and Non-Human Primates Publisher



Alimohammadi R1 ; Porgoo M2 ; Eftekhary M3 ; Kiaie SH4 ; Ansari Dezfouli E4 ; Dehghani M2 ; Nasrollahi K5 ; Malekshahabi T5 ; Heidari M1 ; Pouya S1 ; Alimohammadi M1 ; Sattari Khavas D6 ; Modaresi MS5 ; Ghasemi MH2 Show All Authors
Authors
  1. Alimohammadi R1
  2. Porgoo M2
  3. Eftekhary M3
  4. Kiaie SH4
  5. Ansari Dezfouli E4
  6. Dehghani M2
  7. Nasrollahi K5
  8. Malekshahabi T5
  9. Heidari M1
  10. Pouya S1
  11. Alimohammadi M1
  12. Sattari Khavas D6
  13. Modaresi MS5
  14. Ghasemi MH2
  15. Ramyar H2
  16. Mohammadipour F3
  17. Hamzelouei F7
  18. Mofayezi A8
  19. Mottaghi SS8
  20. Rahmati A8
  21. Razzaznian M8
  22. Tirandazi V3
  23. Tat M5
  24. Borzouee F8
  25. Sadeghi H8
  26. Haji Mohammadi M9
  27. Rastegar L9
  28. Safar Sajadi SM9
  29. Ehsanbakhsh H2
  30. Bazmbar H2
  31. Baghernejadan Z1
  32. Shams Nouraei M4
  33. Pazooki P3
  34. Pahlavanneshan M4
  35. Alishah K5
  36. Nasiri F3
  37. Mokhberian N3
  38. Mohammadi SS4
  39. Akar S10
  40. Niknam H10
  41. Azizi M8
  42. Ajoudanian M6
  43. Moteallehiardakani MH8
  44. Mousavi Shaegh SA10, 11
  45. Ramezani R4, 12
  46. Salimi V13
  47. Moazzami R8
  48. Hashemi SM14
  49. Dehghanizadeh S15
  50. Khoddami V15, 16
Show Affiliations
Authors Affiliations
  1. 1. Department of Immunology, ReNAP Therapeutics, Tehran, Iran
  2. 2. Department of Process Engineering, ReNAP Therapeutics, Tehran, Iran
  3. 3. Department of Quality Control, ReNAP Therapeutics, Tehran, Iran
  4. 4. Department of Formulation Development, ReNAP Therapeutics, Tehran, Iran
  5. 5. Department of Genetic Engineering, ReNAP Therapeutics, Tehran, Iran
  6. 6. Department of Engineering, ReNAP Therapeutics, Tehran, Iran
  7. 7. Department of Quality Assurance, ReNAP Therapeutics, Tehran, Iran
  8. 8. Department of Protein Engineering, ReNAP Therapeutics, Tehran, Iran
  9. 9. Department of Chemistry, ReNAP Therapeutics, Tehran, Iran
  10. 10. Rizsamaneh Behboud Darman, Mashhad Medical Technologies Science Park, Mashhad, Iran
  11. 11. Laboratory of Microfluidics and Medical Microsystems, BuAli Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
  12. 12. Department of Family Therapy, Women Research Center, Alzahra University, Tehran, Iran
  13. 13. Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  14. 14. Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  15. 15. ReNAP Therapeutics, Tehran, Iran
  16. 16. Pediatric Cell and Gene Therapy Research Center, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran

Source: npj Vaccines Published:2022


Abstract

At the forefront of biopharmaceutical industry, the messenger RNA (mRNA) technology offers a flexible and scalable platform to address the urgent need for world-wide immunization in pandemic situations. This strategic powerful platform has recently been used to immunize millions of people proving both of safety and highest level of clinical efficacy against infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here we provide preclinical report of COReNAPCIN®; a vaccine candidate against SARS-CoV-2 infection. COReNAPCIN® is a nucleoside modified mRNA-based vaccine formulated in lipid nanoparticles (LNPs) for encoding the full-length prefusion stabilized SARS-CoV-2 spike glycoprotein on the cell surface. Vaccination of C57BL/6 and BALB/c mice and rhesus macaque with COReNAPCIN® induced strong humoral responses with high titers of virus-binding and neutralizing antibodies. Upon vaccination, a robust SARS-CoV-2 specific cellular immunity was also observed in both mice and non-human primate models. Additionally, vaccination protected rhesus macaques from symptomatic SARS-CoV-2 infection and pathological damage to the lung upon challenging the animals with high viral loads of up to 2 × 108 live viral particles. Overall, our data provide supporting evidence for COReNAPCIN® as a potent vaccine candidate against SARS-CoV-2 infection for clinical studies. © 2022, The Author(s).
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