Nanomicellar-Curcumin Exerts Its Therapeutic Effects Via Affecting Angiogenesis, Apoptosis, and T Cells in a Mouse Model of Melanoma Lung Metastasis Publisher Pubmed



Mardani R¹ ; Hamblin MR² ; Taghizadeh M³ ; Banafshe HR⁴ ; Nejati M⁵ ; Mokhtari M^{6, 7} ; Borran S⁸ ; Davoodvandi A^{3, 9} ; Khan H¹⁰ ; Jaafari MR^{11, 12} ; Mirzaei H³ Show Affiliations
Source: Pathology Research and Practice Published:2020

Abstract

Background: Curcumin is a natural phytochemical polyphenol with significant anti-cancer effects and negligible side effects. In this study, the therapeutic capacity of nanomicellar-curcumin for treating lung metastasis was evaluated in an immunocompetent mouse model of metastatic melanoma. Martials and methods: Two doses of nanomicellar-curcumin (i.e. 10 and 20 μM) were used to induce cytotoxicity in 3 melanoma cell lines. A total of 60 mice were allocated to 20 mice in each of three groups (10 for survival and 10 for assays). Groups were no treatment control, PBS control, nanomicellar-curcumin 20 mg/kg IP 4 times a week, for three weeks). Immunohistochemistry, TUNEL assay, and Western blots were used on lung samples. Results: Nanomicellar-curcumin inhibited the in vitro growth of B16 F10 melanoma cells at 20 μM over 72 h. In vivo, 20 mg/kg nanomicellar-curcumin injected IP, delayed tumor cell growth and significantly extended mouse survival rate. Tumor infiltration of regulatory T cells and angiogenesis were reduced, while IFN-γ and CXCL10 were increased. Conclusion: Nanomicellar-curcumin can inhibit lung metastasis and growing melanoma via activation of apoptosis, activated T cells and inhibition of angiogenesis, tumor growth and regulatory T cells. © 2020 Elsevier GmbH