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The Effect of Bmp-6 Growth Factor on Differentiation of Adipose-Derived Stem Cells Into Chondrocyte in Pellet Culture System



Hashemibeni B1 ; Razavi S1 ; Esfandiary E1 ; Salehi M2 ; Karbasi S3 ; Mardani M1 ; Nadali F4 ; Sadeghi F5 ; Sabahi A1 ; Amuzegar F5 ; Homayuni V6
Authors
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Authors Affiliations
  1. 1. Department of Anatomy, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Pathology and Hematology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  5. 5. School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  6. 6. Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2009

Abstract

Background: Cartilage damaged by trauma or degenerative arthritis has a limited capacity for regeneration most likely because of its avascularity and low cellularity. In this study, adipose-derived stem cells were cultured in chondrogenic medium supplemented isolated from adipose tissue then were induced for chondrogenesis with BMP-6 in Pellet culture system. Methods: Human adipose tissues obtained from cesarean section and ADSCs were isolated and characterized by flowcytometry techniques for CD14, CD44, CD45, and CD90 markers. To induct of chondrogenesis ADSCs were cultured in Pellet culture for 21 days in chondrogenic medium supplemented with BMP-6. Resulted constructs were investigated by histological, immunohistochemical and RT-PCR methods. Findings: ADSCs were expressed CD44, CD90 and not CD14 or CD45. ADSCs chondrogenesis was occurred successfully. Stained-sections in H-E and alcian blue were showed chondrocytes in lacuna with sulfated proteoglycans extracellular matrix. Existence of collagen type-II and aggrecan was proved in constructs by immunohistochemical study. RT-PCR method was exhibited collagen type-II and aggrecan genes expression in differentiated cells. Conclusion: Human adipose-derived stem cells are excellent candidate for cartilage tissue engineering. In Pellet culture ADSCs in presence of BMP-6 were induced for chondrogenesis. © 2009, Isfahan University of Medical Sciences(IUMS). All Rights Reserved.
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