Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Silk Fibroin/Alumina Nanoparticle Scaffold Using for Osteogenic Differentiation of Rabbit Adipose-Derived Stem Cells Publisher



Zafar B1 ; Mottaghitalab F2 ; Shahosseini Z3 ; Negahdari B1 ; Farokhi M4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Medical Biotechnology, Faculty of Advance Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Molecular virology department, Pasteur institute of Iran, Tehran, Iran
  4. 4. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran

Source: Materialia Published:2020


Abstract

In the present study, blended silk fibroin (SF)/alumina nanoparticles were prepared to assess the osteogenic differentiation of rabbit adipose-derived stem cells (rADSCs). The structural and biological behaviours of the nanocomposite were evaluated. Themicro-porous structure of SF/alumina nanocomposite had acceptable mechanical strength, water uptake capacity, and biomineralization capability. Increasing the content of alumina nanoparticles in the structure of SF substrate more than 10% enhanced the physiochemical properties of the bulk SF scaffold without any toxicity effect on the proliferation and attachment of rADSCs. More alkaline phosphates activity and calcium phosphate deposition were also observed in nanocomposite with 10% alumina nanoparticle. Compare with other groups, applying the blended scaffold along with osteogenic medium induced rADSCs to express higher osteogenic markers like collagen type I, osteopontin, RUNX2, and osteocalcin. Taken together, SF/alumina nanocomposite serves as good substrates for proliferation and osteogenic differentiation of ADSCs applicable for bone tissue engineering. © 2019 Acta Materialia Inc.
Other Related Docs
13. Evaluation of Bioactivity and Biocompatibility of Silk Fibroin/Tio2 Nanocomposite, Journal of Medical and Biological Engineering (2018)
15. Recent Trends in Controlled Drug Delivery Based on Silk Platforms, Silk-Based Biomaterials for Tissue Engineering# Regenerative# and Precision Medicine# 2nd Edition (2023)
16. Importance of Dual Delivery Systems for Bone Tissue Engineering, Journal of Controlled Release (2016)
20. In Vivo Assessment of a Nanofibrous Silk Tube As Nerve Guide for Sciatic Nerve Regeneration, Artificial Cells# Nanomedicine and Biotechnology (2018)
25. Thermo-Sensitive Polymers in Medicine: A Review, European Polymer Journal (2019)