Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Design, Synthesis, and Comparison of Pla-Peg-Pla and Peg-Pla-Peg Copolymers for Curcumin Delivery to Cancer Cells Publisher



Rostami N1 ; Faridghiasi F2 ; Ghebleh A3 ; Noei H4 ; Samadzadeh M5 ; Gomari MM6 ; Tajiki A1 ; Abdouss M1 ; Aminoroaya A7 ; Kumari M7 ; Heidari R8 ; Uversky VN9 ; Smith BR7
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Chemistry, Amirkabir University of Technology, Tehran, 1591634311, Iran
  2. 2. Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, 1449614535, Iran
  3. 3. School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, 8174673461, Iran
  4. 4. Department of Medical Biology and Genetics, Faculty of Medicine, Istinye University, Istanbul, 34010, Turkey
  5. 5. Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Istinye University, Istanbul, 34010, Turkey
  6. 6. Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, 1449614535, Iran
  7. 7. Department of Biomedical Engineering and Institute for Quantitative Health Science and Engineering, Michigan State University, East Lansing, 48824, MI, United States
  8. 8. Research Center for Cancer Screening and Epidemiology, AJA University of Medical Sciences, Tehran, 1411718541, Iran
  9. 9. Department of Molecular Medicine, USF Health Byrd Alzheimer’s Research Institute, Morsani College of Medicine, University of South Florida, Tampa, 33612, FL, United States

Source: Polymers Published:2023


Abstract

Curcumin (CUR) has potent anticancer activities, and its bioformulations, including biodegradable polymers, are increasingly able to improve CUR’s solubility, stability, and delivery to cancer cells. In this study, copolymers comprising poly (L-lactide)-poly (ethylene glycol)-poly (L-lactide) (PLA-PEG-PLA) and poly (ethylene glycol)-poly (L-lactide)-poly (ethylene glycol) (PEG-PLA-PEG) were designed and synthesized to assess and compare their CUR-delivery capacity and inhibitory potency on MCF-7 breast cancer cells. Molecular dynamics simulations and free energy analysis indicated that PLA-PEG-PLA has a higher propensity to interact with the cell membrane and more negative free energy, suggesting it is the better carrier for cell membrane penetration. To characterize the copolymer synthesis, Fourier transform-infrared (FT-IR) and proton nuclear magnetic resonance (1H-NMR) were employed, copolymer size was measured using dynamic light scattering (DLS), and their surface charge was determined by zeta potential analysis. Characterization indicated that the ring-opening polymerization (ROP) reaction was optimal for synthesizing high-quality polymers. Microspheres comprising the copolymers were then synthesized successfully. Of the two formulations, PLA-PEG-PLA experimentally exhibited better results, with an initial burst release of 17.5%, followed by a slow, constant release of the encapsulated drug up to 80%. PLA-PEG-PLA-CUR showed a significant increase in cell death in MCF-7 cancer cells (IC50 = 23.01 ± 0.85 µM) based on the MTT assay. These data were consistent with gene expression studies of Bax, Bcl2, and hTERT, which showed that PLA-PEG-PLA-CUR induced apoptosis more efficiently in these cells. Through the integration of nano-informatics and in vitro approaches, our study determined that PLA-PEG-PLA-CUR is an optimal system for delivering curcumin to inhibit cancer cells. © 2023 by the authors.
Related Docs
View other Related Docs
1. Peptide-Functionalized Polymeric Nanoparticles for Delivery of Curcumin to Cancer Cells, Journal of Drug Delivery Science and Technology (2024)
2. Synthesis and in Vitro/In Vivo Characterization of Raloxifene Grafted Poly(Styrene Maleic Acid)-Poly(Amide-Ether-Ester-Imide) Micelles for Targeted Delivery of Docetaxel in G Protein-Coupled Estrogen Receptor Breast Cancer, Anti-Cancer Agents in Medicinal Chemistry (2018)
3. Co-Delivery of Arsenotungstate Polyoxometalate and Curcumin Using Bis-Imidazolium Poly Ionic Liquid Nanocomposites for Boosting Anticancer Effects Against Triple-Negative Breast Cancer Cells, Journal of Inorganic and Organometallic Polymers and Materials (2024)
4. Antitumor Activity of Raloxifene-Targeted Poly(Styrene Maleic Acid)-Poly (Amide-Ether-Ester-Imide) Co-Polymeric Nanomicelles Loaded With Docetaxel in Breast Cancer-Bearing Mice, Investigational New Drugs (2018)
5. Efficient Biocompatible Drug Carrier: Lignin-Based Nanogel Amplifies Curcumin's Cytotoxic Effects and Induces Apoptosis in Brain and Lung Cancer Cell Lines, Biocatalysis and Agricultural Biotechnology (2024)
6. Thermosensitive Folic Acid-Targeted Poly (Ethylene-Co-Vinyl Alcohol) Hemisuccinate Polymeric Nanoparticles for Delivery of Epirubicin to Breast Cancer Cells, Iranian Polymer Journal (English Edition) (2016)
7. Synthesis, in Vitro Characterization, and Anti-Tumor Effects of Novel Polystyrene-Poly(Amide-Ether-Ester-Imide) Co-Polymeric Micelles for Delivery of Docetaxel in Breast Cancer in Balb/C Mice, Drug Development and Industrial Pharmacy (2018)
8. Plga-Peg-Ra-Based Polymeric Micelles for Tumor Targeted Delivery of Irinotecan, Pharmaceutical Development and Technology (2018)
Experts (# of related papers)
View all Related Experts
Jaleh Varshosaz (47)
Farshid Hassanzadeh (30)
Other Related Docs
9. Hyaluronic Acid Targeted Metal Organic Framework Based on Iron (Iii) for Delivery of Platinum Curcumin Cytotoxic Agent to Triple Negative Breast Cancer Cell Line, Applied Organometallic Chemistry (2022)
10. Targeted Nanostructured Lipid Carriers for Delivery of Paclitaxel to Cancer Cells: Preparation, Characterization, and Cell Toxicity, Current Drug Delivery (2017)
11. In Vitro and in Vivo Evaluation of Novel Dtx-Loaded Multifunctional Heparin-Based Polymeric Micelles Targeting Folate Receptors and Endosomes, Recent Patents on Anti-Cancer Drug Discovery (2020)
12. Supramolecular Self-Assembled Nanogels a New Platform for Anticancer Drug Delivery, Current Pharmaceutical Design (2017)
13. Folated Synperonic-Cholesteryl Hemisuccinate Polymeric Micelles for the Targeted Delivery of Docetaxel in Melanoma, BioMed Research International (2015)
14. Co-Delivery of Dasatinib and Mir-30A by Liposomes Targeting Neuropilin-1 Receptors for Triple-Negative Breast Cancer Therapy, Journal of Materials Chemistry B (2024)
15. Transferrin-Targeted Poly(Butylene Adipate)/Terephthalate Nanoparticles for Targeted Delivery of 5-Fluorouracil in Ht29 Colorectal Cancer Cell Line, Journal of Bioactive and Compatible Polymers (2017)
16. Gene Delivery by Pei-Nanocomplex Into Breast and Colorectal Tumor Cell Lines, the Impacts of N/P Ratio, Size and Type of the Cell; [Pei-Nanocomplex Tarafindan Meme Ve Kolorektal Tumor Hucre Hatlarinda Gen Dagitimi, N / P Oraninin, Hucrenin Boyutu Ve Turunun Etkileri], Fabad Journal of Pharmaceutical Sciences (2020)
17. A Concise Review on Cancer Treatment Methods and Delivery Systems, Journal of Drug Delivery Science and Technology (2019)
18. Mesoporous Silica@Chitosan@Gold Nanoparticles As “On/Off” Optical Biosensor and Ph-Sensitive Theranostic Platform Against Cancer, International Journal of Biological Macromolecules (2022)
19. Antibacterial Host-Guest Intercalated Ldh-Adorned Polyurethane for Accelerated Dermal Wound Healing, ACS Applied Bio Materials (2022)
20. Dendrimers As Nanoscale Vectors: Unlocking the Bars of Cancer Therapy, Seminars in Cancer Biology (2022)
21. Biotin-Encoded Pullulan-Retinoic Acid Engineered Nanomicelles: Preparation, Optimization and in Vitro Cytotoxicity Assessment in Mcf-7 Cells, Indian Journal of Pharmaceutical Sciences (2016)
22. Development and Optimization of Transferrin-Conjugated Nanostructured Lipid Carriers for Brain Delivery of Paclitaxel Using Box–Behnken Design, Pharmaceutical Development and Technology (2017)
23. Magnetic Polyvinyl Caprolactam–Polyvinyl Acetate–Polyethylene Glycol Micelles for Docetaxel Delivery in Breast Cancer: An in Vitro Study on Two Cell Lines of Breast Cancer, Pharmaceutical Development and Technology (2017)
24. Encapsulation of Bioactives Within Electrosprayed Κ-Carrageenan Nanoparticles, Carbohydrate Polymers (2022)
25. An Injectable Thermosensitive Hydrogel/Nanomicelles Composite for Local Chemo-Immunotherapy in Mouse Model of Melanoma, Journal of Biomaterials Applications (2022)
26. Protein-Polysaccharide Interactions for the Fabrication of Bioactive-Loaded Nanocarriers: Chemical Conjugates and Physical Complexes, Pharmacological Research (2022)
27. Pharmacokinetics, Organ Toxicity and Antitumor Activity of Docetaxel Loaded in Folate Targeted Cholesterol Based Micelles, Current Drug Delivery (2016)
28. Anticancer Nano-Delivery Systems Based on Bovine Serum Albumin Nanoparticles: A Critical Review, International Journal of Biological Macromolecules (2021)
29. Synthesis and Characterization of Folate-Targeted Dextran/Retinoic Acid Micelles for Doxorubicin Delivery in Acute Leukemia, BioMed Research International (2014)
30. Ph-Responsive Glucosamine Anchored Polydopamine Coated Mesoporous Silica Nanoparticles for Delivery of Anderson-Type Polyoxomolybdate in Breast Cancer, Journal of Microencapsulation (2022)
31. Cancer Stem Cells (Cscs): The Blockage of Metastatic and Stemness Properties by Metal Nanoparticles, Nanomedicine Research Journal (2021)
32. Graphene Oxide Quantum Dot-Chitosan Nanotheranostic Platform As a Ph-Responsive Carrier for Improving Curcumin Uptake Internalization: In Vitro & in Silico Study, Biomaterials Advances (2022)
33. Pharmacokinetics and in Vitro/In Vivo Antitumor Efficacy of Aptamer-Targeted Ecoflex® Nanoparticles for Docetaxel Delivery in Ovarian Cancer, International Journal of Nanomedicine (2018)
34. Peptide-Based Pegylated Polyurethane Nanoparticles for Paclitaxel Delivery in Hela Cancer Cells: The Art of the Architecture Design in Nanocarriers, Polymer Bulletin (2023)
35. Synthesis of Biotin-Targeted Chitosan/Poly (Methyl Vinyl Ether- Alt -Maleic Acid) Copolymeric Micelles for Delivery of Doxorubicin, IET Nanobiotechnology (2017)
36. Cytotoxic Effects of Targeted Galactosylated Pluronic F127/Pluronicl122 Nano-Sized Micelles Loaded With Doxorubicin in Human Hepatocellular Carcinoma Cell Line, Journal of Isfahan Medical School (2013)
37. Evaluation of Thermosensitive Chitosan Hydrogel Containing Gefitinib Loaded Cellulose Acetate Butyrate Nanoparticles in a Subcutaneous Breast Cancer Model, International Journal of Pharmaceutics (2022)
38. Cytotoxic Effect of Pluronic F127-Poly (Methyl Vinyl Ether-Alt-Maleic Acid) Micelles Loaded With Doxorubicin on Mcf-7 Cells, Journal of Isfahan Medical School (2013)
39. Curcumin: A New Candidate for Melanoma Therapy?, International Journal of Cancer (2016)
40. Poly (Butylene Adipate-Co-Terephthalate) Electrospun Nanofibers Loaded With 5-Fluorouracil and Curcumin in Treatment of Colorectal Cancer Cells, Polymer Testing (2018)
41. Biotin Decorated Sunitinib Loaded Nanostructured Lipid Carriers for Tumor Targeted Chemotherapy of Lung Cancer, Journal of Drug Delivery Science and Technology (2019)
42. Tissue Distribution and Systemic Toxicity Evaluation of Raloxifene Targeted Polymeric Micelles of Poly (Styrene-Maleic Acid)-Poly (Amide- Ether-Ester-Imide)-Poly (Ethylene Glycol) Loaded With Docetaxel in Breast Cancer Bearing Mice, Recent Patents on Anti-Cancer Drug Discovery (2019)
43. Fluorescence Resonance Energy Transfer Monitoring of Ph-Responsive Doxorubicin Release From Carbon Dots/Aptamer Functionalized Magnetic Mesoporous Silica, Nanomedicine (2021)
44. Anti-Cancer Activity of Biotin-Polyoxomolybdate Bioconjugate, Eurasian Journal of Medicine and Oncology (2020)
45. Co-Delivery of Carboplatin and Doxorubicin Using Zif-8 Coated Chitosan-Poly(N-Isopropyl Acrylamide) Nanoparticles Through a Dual Ph/Thermo Responsive Strategy to Breast Cancer Cells, International Journal of Biological Macromolecules (2024)
46. Co-Delivery of Paclitaxel and Α-Tocopherol Succinate by Novel Chitosan-Based Polymeric Micelles for Improving Micellar Stability and Efficacious Combination Therapy, Drug Development and Industrial Pharmacy (2015)
47. Luteinizing Hormone-Releasing Hormone Targeted Poly(Methyl Vinyl Ether Maleic Acid) Nanoparticles for Doxorubicin Delivery to Mcf-7 Breast Cancer Cells, IET Nanobiotechnology (2016)
48. Curcumin Nanoparticles Containing Poloxamer or Soluplus Tailored by High Pressure Homogenization Using Antisolvent Crystallization, International Journal of Pharmaceutics (2019)
49. Hierarchical Multifunctional Graphene Oxide Cancer Nanotheranostics Agent for Synchronous Switchable Fluorescence Imaging and Chemical Therapy, Microchimica Acta (2020)
50. A Novel Mixed Polymeric Micelle for Co-Delivery of Paclitaxel and Retinoic Acid and Overcoming Multidrug Resistance: Synthesis, Characterization, Cytotoxicity, and Pharmacokinetic Evaluation, Drug Development and Industrial Pharmacy (2018)